羊传染性脓疱病毒感染山羊皮肤成纤维上皮细胞差异表达miRNA分析

doi:10.11843/j.issn.0366-6964.2020.08.017

Abstract

Abstract: To study the effects of orfv infection on different miRNA expression profile in goat skin fibroblasts (GSF) cells, and understand the roles and regulatory mechanisms of miRNA during orfv infection, total RNA was isolated from GSF cells with or without orfv infection. Differential expressed miRNAs were obtained and analyzed with high-throughput sequencing technology after the miRNA library being constructed. The target genes of differential expressed miRNA were predicted and analyzed by GO and KEGG methods. Ten different miRNAs were randomly selected for RT-qPCR verification. Results indicated that there were total 678 differential expressed miRNAs (fold change≥1.5) between orfv infected and control group, 509 were up-regulated miRNAs, 169 were down-regulated miRNAs, and only 8.21% were conjunct miRNAs between infection group and control group according to the Venn diagram analysis of uniq_miRNA. GO and KEGG analysis showed that the differentially expressed miRNAs were mainly involved in cell biological processes such as lipid metabolism, receptor and cytokine signal transduction. RT-qPCR results of miRNA were consistent with high-throughput sequencing results. In a word, this study obtained a large number of different miRNAs encoded by GSF cells and it indicated that infection of GSF cells with orfv has a significant impact on the miRNA, which provide a reference for further revealing the infection and pathogenesis mechanisms of orfv at the host cells miRNA level.

Key words: ovine contagious pustular dermatitis, goat skin fibroblast, orf virus infection, differential expressed miRNA

CLC Number:

S852.659.1

ZHANG Dajun, HOU Jing, SHEN Chaochao, XU Guowei, KONG Hanjin, CHENG Weiwei, ZHENG Haixue, LIU Xiangtao, ZHANG Keshan. Differential Expression Analysis of miRNA from Orf Virus Infected and Uninfected Goat Skin Fibroblast Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(8): 1932-1938.

References 34

[1]	ZHANG K S, LIU Y J, KONG H J, et al. Human infection with ORF virus from goats in China, 2012[J]. Vector Borne Zoonotic Dis, 2014, 14(5):365-367.
[2]	FLORES C, GONZÁLEZ E, VERNA A, et al. Orf virus in human, confirmation in case report from Chile[J]. Rev Chil Infectol, 2017, 34(6):607-609.
[3]	ANDREANI J, FONGUE J, KHALIL J Y B, et al. Human infection with orf virus and description of its whole genome, France, 2017[J]. Emerg Infect Dis, 2019, 25(12):2197-2204.
[4]	Centers for Disease Control and Prevention. Human orf virus infection from household exposures-United States, 2009-2011[J]. MMWR Morb Mortal Wkly Rep, 2012, 61(14):245-248.
[5]	DEMIRASLAN H, DINC G, DOGANAY M. An overwiev of ORF virus infection in humans and animals[J]. Recent Pat Antiinfect Drug Discov, 2017, 12(1):21-30.
[6]	ZHANG K S, LIU Y J, KONG H J, et al. Comparison and phylogenetic analysis based on the B2L gene of orf virus from goats and sheep in China during 2009-2011[J]. Arch Virol, 2014, 159(6):1475-1479.
[7]	SCHNEIDER L E, PROTSCHKA M, MÜLLER U, et al. Orf virus infection of human keratinocytes and dermal fibroblasts:limited virus detection and interference with intercellular adhesion molecule-1 up-regulation[J]. Exp Dermatol, 2019, 28(2):142-151.
[8]	HAIG D M, MCINNES C J. Immunity and counter-immunity during infection with the parapoxvirus orf virus[J]. Virus Res, 2002, 88(1-2):3-16.
[9]	BERGQVIST C, KURBAN M, ABBAS O. Orf virus infection[J]. Rev Med Virol, 2017, 27(4):e1932.
[10]	CARAVAGLIO J V, KHACHEMOUNE K. Orf virus infection in humans:a review with a focus on advances in diagnosis and treatment[J]. J Drugs Dermatol, 2017, 16(7):684-689.
[11]	YAZBECK A M, STADLER P F, TOUT K, et al. Automatic curation of large comparative animal microRNA datasets[J]. Bioinformatics, 2019, 35(22):4553-4559.
[12]	KITTELMANN S, MCGREGOR A P. Modulation and evolution of animal development through microRNA regulation of gene expression[J]. Genes (Basel), 2019, 10(4):321.
[13]	NAVEED A, UR-RAHMAN S, ABDULLAH S, et al. A concise review of microRNA exploring the insights of microRNA regulations in bacterial, viral and metabolic diseases[J]. Mol Biotechnol, 2017, 59(11-12):518-529.
[14]	WU J J, JI Z Y, QIAO M, et al. microRNA transcriptome analysis of poly I:C-stimulated and PRRSV-infected porcine alveolar macrophages[J]. J Appl Genet, 2019, 60(3-4):375-383.
[15]	LIU F, WANG H L, DU L, et al. microRNA-30c targets the interferon-alpha/beta receptor beta chain to promote type 2 PRRSV infection[J]. J Gen Virol, 2018, 99(12):1671-1680.
[16]	WANG X, ZHANG M M, YAN K, et al. The full-length microRNA cluster in the intron of large latency transcript is associated with the virulence of pseudorabies virus[J]. Virology, 2018, 520:59-66.
[17]	LI Y T, ZHENG G M, ZHANG Y J, et al. microRNA analysis in mouse neuro-2a cells after pseudorabies virus infection[J]. J Neurovirol, 2017, 23(3):430-440.
[18]	SHAHEN M, GUO Z H, SHAR A H, et al. Dengue virus causes changes of MicroRNA-genes regulatory network revealing potential targets for antiviral drugs[J]. BMC Syst Biol, 2018, 12(1):2.
[19]	OSPINA-BEDOYA M, CAMPILLO-PEDROZA N, FRANCO-SALAZAR J P, et al. Computational identification of dengue virus microRNA-like structures and their cellular targets[J]. Bioinform Biol Insights, 2014, 8:169-176.
[20]	NABIEL Y, ELSHAHAWY H, MOSBAH A. Intrauterine bacterial colonization and endometrial microRNA-17-5p levels in association to endometriosis:a study in an egyptian population[J]. Immunol Invest:A J Mol Cell Immunol, 2019, doi:10. 1080/08820139. 2019. 1693592.
[21]	ZHAO C H, ZHOU Z, ZHANG T F, et al. Salmonella small RNA fragment Sal-1 facilitates bacterial survival in infected cells via suppressing iNOS induction in a microRNA manner[J]. Sci Rep, 2017, 7(1):16979.
[22]	KAMENETZKY L, STEGMAYER G, MALDONADO L, et al. MicroRNA discovery in the human parasite Echinococcus multilocularis from genome-wide data[J]. Genomics, 2016, 107(6):274-280.
[23]	MACCHIAROLI N, CUCHER M, ZAROWIECKI M, et al. microRNA profiling in the zoonotic parasite Echinococcus canadensis using a high-throughput approach[J]. Parasit Vectors, 2015, 8:83.
[24]	LIVAK K J, SCHMITTGEN T D. Analysis of relative gene expression data using real-time quantitative PCR and the 2-ΔΔCT method[J]. Methods, 2001, 25:402-408.
[25]	LONG M J, WANG Y Y, CHEN D X, et al. Identification of host cellular proteins LAGE3 and IGFBP6 that interact with orf virus protein ORFV024[J]. Gene, 2018, 661:60-67.
[26]	COUÑAGO R M, KNAPP K M, NAKATANI Y, et al. Structures of orf virus chemokine binding protein in complex with host chemokines reveal clues to broad binding specificity[J]. Structure, 2015, 23(7):1199-213.
[27]	TIAN H, CHEN Y, WU J Y, et al. Identification and function analysis of the host cell protein that interacted with Orf virus Bcl-2-like protein ORFV125[J]. Res Vet Sci, 2016, 108:93-97.
[28]	CHEN L Y, MING X Y, LI W S, et al. The microRNA-155 mediates hepatitis B virus replication by reinforcing SOCS1 signalling-induced autophagy[J]. Cell Biochem Funct, 2020, doi:doi. org/10. 1002/cbf. 3488.
[29]	NATEKAR J P, ROTHAN H A, ARORA K, et al. Cellular microRNA-155 regulates virus-induced inflammatory response and protects against lethal West Nile virus infection[J]. Viruses, 2019, 12(1):9.
[30]	ZHENG B J, ZHOU J M, WANG H. Host micro-RNAs and exosomes that modulate influenza virus infection[J]. Virus Res, 2020, 279:197885.
[31]	CUI H, ZHANG C M, ZHAO Z Z, et al. Identification of cellular microRNA miR-188-3p with broad-spectrum anti-influenza A virus activity[J]. Virol J, 2020, 17(1):12.
[32]	WONG R R, ABD-AZIZ N, AFFENDI S, et al. Role of microRNAs in antiviral responses to dengue infection[J]. J Biomed Sci, 2020, 27:4.
[33]	QI L L, WANG K L, CHEN H T, et al. Host microRNA miR-1307 suppresses foot-and-mouth disease virus replication by promoting VP3 degradation and enhancing innate immune response[J]. Virology, 2019, 535:162-170.
[34]	SODROSKI C, LOWEY B, HERTZ L, et al. microRNA-135a modulates hepatitis C virus genome replication through downregulation of host antiviral factors[J]. Virol Sin, 2019, 34(2):197-210.

Differential Expression Analysis of miRNA from Orf Virus Infected and Uninfected Goat Skin Fibroblast Cells

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