Muscle cells (also known as muscle fibers) are the basic unit of skeletal muscle, accounting for about 50% of the body of livestock and poultry, and are closely related to meat production performance and bone protection of livestock and poultry. They play an important role in regulating the metabolism and endocrine of livestock and poultry, and also provide power for the movement of livestock and poultry. Skeletal muscle is a relatively complex tissue, and the basic unit of these tissues are muscle cells. Skeletal muscle requires a variety of types of mononuclear and multinucleated cells to interact during development, which is the key to the formation of high-quality meat. In recent years, with the rapid development of science and technology, there are more and more comprehensive techniques for identifying skeletal muscle development-related cells, and single cell sequencing technology is most widely used. Its application provides technical support for the study of cell types and related cell development heterogeneity of skeletal muscle, and is of great significance for the study of meat quality. This paper reviews the identification of cells related to skeletal muscle development by cell morphology observation, molecular markers, immunohistochemistry, immunofluorescence, in situ hybridization, and single cell sequencing, and summarizes the marker genes of cells related to skeletal muscle development, in order to understand the structure and function of skeletal muscle. It provides a reference for exploring the mechanism of skeletal muscle development, provides a theoretical basis for the study of meat quality, and also has important significance and practical value in individual breeding of different varieties.