2020—2022年湖北省生猪屠宰场伪狂犬病病毒的分离鉴定及遗传进化分析

doi:10.11843/j.issn.0366-6964.2023.07.028

Abstract

Abstract: The aim of this study was to monitor the prevalence of pseudorabies virus (PRV) in pig slaughterhouses in Hubei Province. A total of 1 795 blood samples and 2 081 lung samples (lung and hilar lymph nodes) were collected from slaughterhouses of different sizes in nine cities in four regions (East Hubei, North Hubei, West Hubei, Central Hubei) of Hubei Province from 2020 to 2022, and antibodies in serum and pathogens in lung samples were detected by ELISA and PCR targeting the PRV gE gene. Virus isolation and identification, whole genome sequencing, and phylogenetic analysis were performed on positive tissue samples. Serological detection results showed that 104 serum samples were positive, with an average positive detection rate of 5.79%. The results of pathogen detection showed that 82 lung samples were positive, with an average positive detection rate of 3.94%. According to different factors, The highest detection rates of antibody and pathogen were 18.57% and 9.74% respectively in spring. The antibody detection rate of eastern Hubei was the highest (10.77%), and the pathogen detection rate of central Hubei was the highest (4.93%). The detection rates of pathogens and antibodies in grade A small slaughterhouses were significantly higher than those in the other three types, reaching 16.28% and 6.01%, respectively. A total of 17 PRV strains were isolated from PRV positive samples. The results of genetic evolution analysis of whole genome and gB, gC and gE genes showed that the isolates belonged to genotype Ⅱ variants, which were in different genetic evolution branches from early foreign isolates such as Bartha, NIA-3 and Becker. HBXG is suspected to be a recombinant strain of genotype Ⅰ and genotype Ⅱ, and is in the same clade2.2 genetic branch as the earlier reported recombinant HuB1/CHN2017. In conclusion, mutant strains were mainly prevalent in pig farms associated with slaughterhouses in Hubei Province. At the same time, recombination and mutation of vaccine strains and wild viruses were still occurring in vaccinated pig farms. Therefore, this study is of great significance for the continuous monitoring of PRV and development of novel vaccines.

Key words: pseudorabies virus, pig slaughterhouses, serological detection, pathogen detection, phylogenetic analysis

CLC Number:

S852.659.1

CHEN Hongjian, CAO Yan, FAN Jie, GAN Rongxuan, SONG Wenbo, YU Shengwei, YANG Ting, ZHAO Yanxia, WEI Chunyan, XIE Rui, HUA Lin, PENG Zhong, WU Bin. Prevalence and Phylogenetic Analysis of Pseudorabies Virus within Pig Slaughterhouses in Hubei Province of China during 2020-2022[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2972-2981.

References 35

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Prevalence and Phylogenetic Analysis of Pseudorabies Virus within Pig Slaughterhouses in Hubei Province of China during 2020-2022

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