影响非洲猪瘟病毒对培养细胞感染性的因素分析

doi:10.11843/j.issn.0366-6964.2023.08.025

Abstract

Abstract: Porcine primary alveolar macrophages (PAMs) were the main target cells of African swine fever virus (ASFV), and it was difficult for ASFV to grow and multiply continuously in other in vitro cell lines except host monocytes/macrophages. The aim of this study was to investigate the factors affecting ASFV in in vitro passaging cultures. By comparing the genome-wide transcriptional profile differences between ASFV inoculated susceptible cells PAMs and non-susceptible cell lines 3D4/21 and PK15 cells; Using small molecule drugs to alter cell metabolism or cycle, physical methods to alter cell adhesion; detecting virus proliferation in 3D4/21 and PK15 cell lines by fluorescence quantitative PCR, Western blot, red cell adsorption. The results showed that:ASFV inoculation of both 3D4/21 and PK15 cells and PAMs cells were significantly enriched in genes common to both cell adhesion, cell cycle and cell metabolism; The ability of ASFV to infect PK15 and 3D4/21 cells was not significantly affected by treatment of cells with various small molecule drugs that regulate cell cycle and cell metabolism; The ability of ASFV to infect PK15 and 3D4/21 cells was significantly affected by suspension culture to regulate The ability of ASFV to infect PK15 and 3D4/21 cells was significantly improved after cell adhesion was regulated by suspension culture, but the replication ability of the virus gradually decreased with the increase in the number of passages. In conclusion, cell adhesion may be one of the important factors affecting the in vitro infection ability of ASFV, but altering cell adhesion did not maintain the replication ability of ASFV. This study provides a preliminary reference for the establishment of ASFV in vitro cell lines.

Key words: African swine fever virus, genome-wide transcriptional profiling, suspension culture, in vitro infected cell line

CLC Number:

S852.659.1

FENG Yongzhi, GONG Ting, WU Dongdong, GAO Qi, ZHENG Xiaoyu, ZHANG Guihong, SUN Yankuo. Analysis of Factors Affecting the Infectivity of African Swine Fever Virus on Cultured Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3406-3414.

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Analysis of Factors Affecting the Infectivity of African Swine Fever Virus on Cultured Cells

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