猫疱疹病毒1型的检测及一株分离毒株的致病性

doi:10.11843/j.issn.0366-6964.2024.05.042

Abstract

Abstract: The aim of this study was to investigate the prevalence and genetic diversity of feline herpesvirus-1 (FHV-1) in Chengdu city. From 2020 to 2023, a total of 178 conjunctiva, pharynx and nose swabs were collected from cats with respiratory symptoms in Chengdu city. The collected samples were subjected to FHV-1 PCR detection, gE gene genetic evolutionary analysis, viral isolation and identification, as well as pathogenicity. The results showed that, the FHV-1 positivity rate in Chengdu city was 11.8% (21/178). In which the FHV-1 positivity rate of veterinary hospitals was 6.4% (7/110) and 20.6% (14/68) in catteries, suggested that the higher rate of FHV-1 infection was in catteries. The genetic evolutionary analysis of five FHV-1 gE genes showed that the nucleotide homology was 99.1%~100% to the vaccine strains,and three unique amino acid mutation sites were found in PX-9 strain (L259Q, C294I, W295F). Five positive samples were isolated and cultured by CRFK cell, only PX-9 strain was successfully isolated and identified from CRFK cells. The results of animal regression experiment by PX-9 strain showed that all cats in the infected group exhibited clinical symptoms, such as increased ocular, nasal secretions and sneezing. The highest viral load was found on the 4th day after inoculation (9.05×10⁷copies·μL^-1), and the detoxification period is relatively long. The results of FHV-1 viral load were 1.7×10⁷ and 3.5×10⁴copies·μg^-1 in trachea and lungs of infected group, respectively. The other tissues were negative for FHV-1 DNA. The results of this study indicated that FHV-1 was still prevalent in Chengdu and the prevalent strains had highly pathogenicity.

Key words: FHV-1, prevalence, genetic variation, animal regression experiment

CLC Number:

DENG Gunan, ZHANG Jiaqi, BAO Zhipeng, CHEN Taoyun, YU Qisheng, DING Lu, ZHU Chenxi, WANG Yi, REN Yupeng, HE Chao, ZHANG Bin. Detection of Feline Herpesvirus Type 1 and Pathogenicity of an Isolated Strain[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2253-2258.

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Detection of Feline Herpesvirus Type 1 and Pathogenicity of an Isolated Strain

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