羊传染性脓疱病毒感染山羊皮肤成纤维上皮细胞差异表达miRNA分析

doi:10.11843/j.issn.0366-6964.2020.08.017

Abstract

Abstract: To study the effects of orfv infection on different miRNA expression profile in goat skin fibroblasts (GSF) cells, and understand the roles and regulatory mechanisms of miRNA during orfv infection, total RNA was isolated from GSF cells with or without orfv infection. Differential expressed miRNAs were obtained and analyzed with high-throughput sequencing technology after the miRNA library being constructed. The target genes of differential expressed miRNA were predicted and analyzed by GO and KEGG methods. Ten different miRNAs were randomly selected for RT-qPCR verification. Results indicated that there were total 678 differential expressed miRNAs (fold change≥1.5) between orfv infected and control group, 509 were up-regulated miRNAs, 169 were down-regulated miRNAs, and only 8.21% were conjunct miRNAs between infection group and control group according to the Venn diagram analysis of uniq_miRNA. GO and KEGG analysis showed that the differentially expressed miRNAs were mainly involved in cell biological processes such as lipid metabolism, receptor and cytokine signal transduction. RT-qPCR results of miRNA were consistent with high-throughput sequencing results. In a word, this study obtained a large number of different miRNAs encoded by GSF cells and it indicated that infection of GSF cells with orfv has a significant impact on the miRNA, which provide a reference for further revealing the infection and pathogenesis mechanisms of orfv at the host cells miRNA level.

Key words: ovine contagious pustular dermatitis, goat skin fibroblast, orf virus infection, differential expressed miRNA

CLC Number:

S852.659.1

ZHANG Dajun, HOU Jing, SHEN Chaochao, XU Guowei, KONG Hanjin, CHENG Weiwei, ZHENG Haixue, LIU Xiangtao, ZHANG Keshan. Differential Expression Analysis of miRNA from Orf Virus Infected and Uninfected Goat Skin Fibroblast Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(8): 1932-1938.

References 34

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Differential Expression Analysis of miRNA from Orf Virus Infected and Uninfected Goat Skin Fibroblast Cells

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