非洲猪瘟病毒D1133 L蛋白单克隆抗体抑制其复制

doi:10.11843/j.issn.0366-6964.2024.02.041

Abstract

Abstract: The aim was to investigate the regulatory effect of a monoclonal antibody against D1133L protein of African swine fever virus (ASFV) during virus replication. Based on the preparation of monoclonal antibody against D1133L protein, ASFV and different concentrations of monoclonal antibodies were added simultaneously to PAMs, and the effect of monoclonal antibody against D1133L on virus replication was analyzed by 50% Hemadsorbing dose (HAD₅₀), real-time quantitative PCR (RT-qPCR), Western blot, and observation of fluorescence. Monoclonal antibodies at different concentrations significantly inhibited the viral titer (P<0.01), the expression level of proteins, the gene transcription level (P<0.01), and the expression level of ASFV-GFP green fluorescent protein (P<0.05) at different infection times in a dose-dependent manner. In summary, monoclonal antibody against D1133L significantly inhibited ASFV replication, the results of the trial are informative in the selection of drug targets for ASFV.

Key words: African swine fever virus, D1133 L protein, monoclonal antibody

CLC Number:

S852.659.1

YAN Wenqian, HOU Jing, YANG Jinke, HAO Yu, YANG Xing, SHI Xijuan, ZHANG Dajun, BIE Xintian, CHEN Guohui, CHEN Lingling, HE Lu, ZHAO Meiyu, ZHAO Siyue, ZHENG Haixue, ZHANG Keshan. Monoclonal Antibody against D1133 L Protein of African Swine Fever Virus Inhibits Its Replication[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 854-859.

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Monoclonal Antibody against D1133 L Protein of African Swine Fever Virus Inhibits Its Replication

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