非洲猪瘟病毒感染相关调控基因以及毒力基因初步筛选

doi:10.11843/j.issn.0366-6964.2023.07.027

Abstract

Abstract: This study aims to preliminarily screen the regulatory genes and virulence genes that interact with the host during African swine fever virus (ASFV) infection, so as to provide a molecular theoretical basis for the development of specific drugs and vaccines against ASFV. We re-analyzed the differentially expressed genes in the host during high and low virulent ASFV strains infection using GSE145954 and literature mining. The Metascape database was used for gene function enrichment analysis and the protein interaction network was established through the STRING database. The key genes in the regulatory network of viral infection host were obtained through network topology screening, and then the ASFV virulence genes regulating the key genes were screened at the cell level. The study found that ECE1, CCL2, CTSB, SCARB2 and CD14 were significantly up-regulated in ASFV infected mononuclear macrophages and whole blood of animals, while HMBS, DYNLL1, UBB, EZH2 and SERPINE1 were significantly down-regulated. PPI analysis showed that UBB, CCL2, CTBB, EZH2, SERPINE1, CD14 and DYNLL1 in host cells are key genes in the regulatory network of host infected with ASFV, in which UBB played a central role. B119L, I215L and MGF360-13L of ASFV can inhibit the expression of UBB. The results suggest that UBB, CCL2, CTSB, EZH2, SERPINE1, CD14 and DYNLL1 are the key genes in the regulatory network, and UBB is the hub gene during ASFV infection. B119L, I215L and MGF360-13L of ASFV inhibit UBB expression, which are important virulent genes of ASFV.

Key words: African swine fever virus, GEO database, protein interaction, UBB, virulent genes

CLC Number:

S852.659.1

DING Xiaoyan, HE Jiuxiang, ZHOU Xiaoyang, ZHOU Yuxin, LI Jintao. Preliminary Identification of Host Regulatory Genes and Virulence Genes during African Swine Fever Virus Infection[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2964-2971.

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Preliminary Identification of Host Regulatory Genes and Virulence Genes during African Swine Fever Virus Infection

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