非洲猪瘟病毒感染相关调控基因以及毒力基因初步筛选

doi:10.11843/j.issn.0366-6964.2023.07.027

Abstract

Abstract: This study aims to preliminarily screen the regulatory genes and virulence genes that interact with the host during African swine fever virus (ASFV) infection, so as to provide a molecular theoretical basis for the development of specific drugs and vaccines against ASFV. We re-analyzed the differentially expressed genes in the host during high and low virulent ASFV strains infection using GSE145954 and literature mining. The Metascape database was used for gene function enrichment analysis and the protein interaction network was established through the STRING database. The key genes in the regulatory network of viral infection host were obtained through network topology screening, and then the ASFV virulence genes regulating the key genes were screened at the cell level. The study found that ECE1, CCL2, CTSB, SCARB2 and CD14 were significantly up-regulated in ASFV infected mononuclear macrophages and whole blood of animals, while HMBS, DYNLL1, UBB, EZH2 and SERPINE1 were significantly down-regulated. PPI analysis showed that UBB, CCL2, CTBB, EZH2, SERPINE1, CD14 and DYNLL1 in host cells are key genes in the regulatory network of host infected with ASFV, in which UBB played a central role. B119L, I215L and MGF360-13L of ASFV can inhibit the expression of UBB. The results suggest that UBB, CCL2, CTSB, EZH2, SERPINE1, CD14 and DYNLL1 are the key genes in the regulatory network, and UBB is the hub gene during ASFV infection. B119L, I215L and MGF360-13L of ASFV inhibit UBB expression, which are important virulent genes of ASFV.

Key words: African swine fever virus, GEO database, protein interaction, UBB, virulent genes

CLC Number:

S852.659.1

DING Xiaoyan, HE Jiuxiang, ZHOU Xiaoyang, ZHOU Yuxin, LI Jintao. Preliminary Identification of Host Regulatory Genes and Virulence Genes during African Swine Fever Virus Infection[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2964-2971.

References 23

[1]	TAKAMATSU H H, DENYER M S, LACASTA A, et al. Cellular immunity in ASFV responses[J]. Virus Res, 2013, 173(1):110-121.
[2]	ESCRIBANO J M, GALINDO I, ALONSO C. Antibody-mediated neutralization of African swine fever virus:myths and facts[J]. Virus Res, 2013, 173(1):101-109.
[3]	REIS A L, NETHERTON C, DIXON L K. Unraveling the armor of a killer:evasion of host defenses by African swine fever virus[J]. J Virol, 2017, 91(6):e02338-16.
[4]	LITHGOW P, TAKAMATSU H, WERLING D, et al. Correlation of cell surface marker expression with African swine fever virus infection[J]. Vet Microbiol, 2014, 168(2-4):413-419.
[5]	FRANZONI G, GRAHAM S P, GIUDICI S D, et al. Characterization of the interaction of African swine fever virus with monocytes and derived macrophage subsets[J]. Vet Microbiol, 2017, 198:88-98.
[6]	NEILAN J G, ZSAK L, LU Z, et al. Neutralizing antibodies to African swine fever virus proteins p30, p54, and p72 are not sufficient for antibody-mediated protection[J]. Virology, 2004, 319(2):337-342.
[7]	GALINDO I, CUESTA-GEIJO M A, HLAVOVA K, et al. African swine fever virus infects macrophages, the natural host cells, via clathrin-and cholesterol-dependent endocytosis[J]. Virus Res, 2015, 200(1):45-55.
[8]	ANDRÉS G. African swine fever virus gets undressed:new insights on the entry pathway[J]. J Virol, 2017, 91(4):e01906-16.
[9]	HERNÁEZ B, GUERRA M, SALAS M L, et al. African swine fever virus undergoes outer envelope disruption, capsid disassembly and inner envelope fusion before core release from multivesicular endosomes[J]. PLoS Pathog, 2016, 12(4):e1005595.
[10]	SÁNCHEZ E G, QUINTAS A, PÉREZ-NÚÑEZ D, et al. African swine fever virus uses macropinocytosis to enter host cells[J]. PLoS Pathog, 2012, 8(6):e1002754.
[11]	REVILLA Y, PÉREZ-NÚÑEZ D, RICHT J A. African swine fever virus biology and vaccine approaches[J]. Adv Virus Res, 2018, 100:41-74.
[12]	GAUDREAULT N N, MADDEN D W, WILSON W C, et al. African swine fever virus:an emerging DNA arbovirus[J]. Front Vet Sci, 2020, 7:215.
[13]	JAING C, ROWLAND R R R, ALLEN J E, et al. Gene expression analysis of whole blood RNA from pigs infected with low and high pathogenic African swine fever viruses[J]. Sci Rep, 2017, 7(1):10115.
[14]	FERREIRA C. Expression of ubiquitin, actin, and actin-like genes in African swine fever virus infected cells[J]. Virus Res, 1996, 44(1):11-21.
[15]	BARRADO-GIL L, GALINDO I, MARTÍNEZ-ALONSO D, et al. The ubiquitin-proteasome system is required for African swine fever replication[J]. PLoS One, 2017, 12(12):e0189741.
[16]	HINGAMP P M, ARNOLD J E, MAYER R J, et al. A ubiquitin conjugating enzyme encoded by African swine fever virus[J]. EMBO J, 1992, 11(1):361-366.
[17]	LEWIS T, ZSAK L, BURRAGE T G, et al. An African swine fever virus ERV1-ALR homologue, 9GL, affects virion maturation and viral growth in macrophages and viral virulence in swine[J]. J Virol, 2000, 74(3):1275-1285.
[18]	O'DONNELL V, HOLINKA L G, KRUG P W, et al. African swine fever virus georgia 2007 with a deletion of virulence-associated gene 9GL (B119L), when administered at low doses, leads to virus attenuation in swine and induces an effective protection against homologous challenge[J]. J Virol, 2015, 89(16):8556-8566.
[19]	GOLDING J P, GOATLEY L, GOODBOURN S, et al. Sensitivity of African swine fever virus to type I interferon is linked to genes within multigene families 360 and 505[J]. Virology, 2016, 493:154-161.
[20]	AFONSO C L, PICCONE M E, ZAFFUTO K M, et al. African swine fever virus multigene family 360 and 530 genes affect host interferon response[J]. J Virol, 2004, 78(4):1858-1864.
[21]	YANG K D, XUE Y, NIU H, et al. African swine fever virus MGF360-11L negatively regulates cGAS-STING-mediated inhibition of type I interferon production[J]. Vet Res, 2022, 53(1):7.
[22]	王洋, 崔帅, 鑫婷, 等. 非洲猪瘟病毒MGF360-14L靶向MAVS抑制Ⅰ型干扰素的产生[J]. 畜牧兽医学报, 2022, 53(9):3272-3278.WANG Y, CUI S, XIN T, et al. ASFV MGF360-14L interacts with MAVS and inhibit the expression of type Ⅰ interferon[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9):3272-3278. (in Chinese)
[23]	陈达年, 马旭升, 代军飞, 等. 非洲猪瘟病毒多基因家族MGF360-13L基因功能的初步研究[J]. 畜牧兽医学报, 2022, 53(12):4419-4428.CHEN D N, MA X S, DAI J F, et al. Preliminary study on the function of MGF360-13L gene of African swine fever virus multigene family[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(12):4419-4428. (in Chinese)

Preliminary Identification of Host Regulatory Genes and Virulence Genes during African Swine Fever Virus Infection

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