抑制USP7活性对鸡抗沙门菌感染的影响

doi:10.11843/j.issn.0366-6964.2022.07.036

Abstract

Abstract: This research aimed to clarify the effect of ubiquitin-specific protease (USP7) on the resistance of chickens to Salmonella Typhimurium infection. Forty 3-day-old White Leghorns were randomly divided into two groups, and respectively intraperitoneal injected with the USP7 inhibitor P5091 and control solvent every other day for 5 consecutive injections (n=20 in each group). Four days after the last injection, they were artificially orally infected with S.Typhimurium. Samples were collected 24 hours after infection. The mRNA abundances of IL-1β, IL-8, TNF-α and NF-κB were examined by RT-qPCR. The bacteria load in the liver of chickens was measured by the plate coating method. Liver and lung inflammation was checked by H&E staining. Inhibition of the USP7 activity by P5091 treatment significantly downregulated the mRNA level of NF-κB, and its downstream cytokines IL-1β, IL-8 and TNF-α. The bacterial load in the liver of chickens decreased. H&E staining showed aggravated inflammation in the liver and lung in USP7 inhibited chickens. USP7 might have critical role in chicken to resist Salmonella infection.

Key words: USP7, Salmonella, immune response, NF-κB, cytokines

CLC Number:

S856.5

ZHANG Na, WANG Fei, GE Ximin, ZHAO Guiping, WEN Jie, LI Qinghe. Correlation between the Expression Level of USP7 and the Immune Response to Salmonella Infection in Chickens[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(7): 2396-2402.

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Correlation between the Expression Level of USP7 and the Immune Response to Salmonella Infection in Chickens

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