沙门菌分子检测方法的研究进展

doi:10.11843/j.issn.0366-6964.2023.08.009

Abstract

Abstract: Salmonella is the most common zoonosis pathogen in Enterobacteriaceae, and Salmonella food poisoning is one of the highest proportions and the most harmful bacterial food poisoning, which has important public health significance. The classification of Salmonella is relatively complex with 2 species (Salmonella enterica is divided into 6 subspecies) according to the biological taxonomy, 46 serogroups and more than 2600 serovars according to the difference of surface antigens (some serovars can be further divided into different biovars). The above classification or typing is of great significance for epidemiological and aetiological research. Traditional detection and serotyping have many defects, such as time-consuming, labor-intensive and low sensitivity, which cannot control the epidemic timely and accurately. With the continuous improvement of Salmonella genomic data, more and more nucleic acid targets have been discovered. The molecular detection technology represented by PCR can not only detect Salmonella, but also identify different serovars, with strong specificity, high sensitivity, simple and fast. The specificity of detection target is the key factor for the accuracy of results. Since 1992, when it was first reported that PCR detection method for Salmonella was established with invA as the target, the reports on molecular detection technology of Salmonella are increased, and it has been gradually applied to the rapid detection and typing of Salmonella. This paper introduces the research progress on molecular detection of Salmonella in detail, and summarizes the application from different levels of Salmonella spp., species, serogroup and serovar, in order to provide reference for the promotion and application of molecular detection of Salmonella.

Key words: Salmonella, serovar, molecular detection technology, detection target

CLC Number:

R378.22

ZHANG Ping, ZHUANG Linlin, ZHANG Di, DONG Yongyi, SHENG Zhongwei, WANG Chengming, XU Bu, DOU Xinhong, GONG Jiansen. Research Progress on Molecular Detection Methods of Salmonella[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3217-3229.

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Research Progress on Molecular Detection Methods of Salmonella

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