生产母兔沙门菌感染的病理学变化及其药物敏感性分析

doi:10.11843/j.issn.0366-6964.2023.08.035

Abstract

Abstract: In order to explore the histopathological characteristics of Salmonella infection in female rabbits, 6 dead female rabbits with depressed, shortness of breath and aborted from a large-scale rabbit farm in Henan province were examined. Endometrial bleeding, stillbirth, and hyperemia and congestion on the surface of heart, liver, spleen, lung and kidney were found by anatomical examination. The isolated pathogen was identified as Salmonella by isolation culture, morphological observation, serological typing, PCR identification, sequencing analysis and pathogenicity test. It was found that the myocardial fibers were bent or arranged disordered, liver cell granular degeneration or steatosis, lung extensive congestion, suppurative hysteritis, endometritis and other organs hyperemia by histopathological observation. The results of drug sensitivity test showed that the pathogens were sensitive to chloramphenicol, cephalosporins, penicillins, quinolones, tetracyclic, polypeptide, β-lactam and sulfa antibiotics, and had certain resistance to aminoglycoside (butamicana). In this study, Salmonella was successfully isolated from rabbits, the histopathological and drug sensitivity analysis of the infected female rabbits were carried out, which provides a basis for better prevention and control of rabbit salmonellosis.

Key words: large-scale rabbit farm, productive female rabbits, Salmonella infection, pathology, susceptibility analysis

CLC Number:

S858.2915.1

ZHANG Qianwen, LIU Yumei, SHI Lihui, LIANG Wenjun, LI Mengyun, WANG Yuqin, ZHANG Ziqiang. Pathological Observation and Drug Sensitivity Analysis of Salmonella Infection in Female Rabbits[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3510-3518.

References 30

[1]	BAO H X, WANG S, ZHAO J H, et al.Salmonella secretion systems:differential roles in pathogen-host interactions[J].Microbiol Res, 2020, 241:126591.
[2]	BERTELLONI F, TOSI G, MASSI P, et al.Some pathogenic characters of paratyphoid Salmonella enterica strains isolated from poultry[J].Asian Pac J Trop Med, 2017, 10(12):1161-1166.
[3]	郭帅, 范志宇, 王芳, 等.家兔肠炎沙门氏菌病流行病学调查及防控[J].中国动物传染病学报, 2019, 27(2):42-47.GUO S, FAN Z Y, WANG F, et al.Epidemiological investigation and prevention of salmonella enteritidis in rabbits[J].Chinese Journal of Animal Infectious Diseases, 2019, 27(2):42-47.(in Chinese)
[4]	范志宇, 魏后军, 仇汝龙, 等.一株新型家兔肠炎沙门氏菌的分离鉴定及防治[J].江苏农业学报, 2016, 32(6):1367-1371.FAN Z Y, WEI H J, QIU R L, et al.Isolation, identification and control of a novel Salmonella enteritidis strain from rabbit[J].Jiangsu Journal of Agricultural Sciences, 2016, 32(6):1367-1371.(in Chinese)
[5]	王高杰.兔肠炎沙门氏菌耐药性及毒力分析[D].南京:南京农业大学, 2019.WANG G J.Analysis of drug resistance and virulence of Salmonella of rabbit enteritis[D].Nanjing:Nanjing Agricultural University, 2019.(in Chinese)
[6]	RUKAMBILE E, SINTCHENKO V, MUSCATELLO G, et al.Infection, colonization and shedding of Campylobacter and Salmonella in animals and their contribution to human disease:a review[J].Zoonoses Public Health, 2019, 66(6):562-578.
[7]	DOS SANTOS A M P, FERRARI R G, CONTE-JUNIOR C A.Virulence factors in Salmonella typhimurium:the sagacity of a bacterium[J].Curr Microbiol, 2019, 76(6):762-773.
[8]	LUK C H, VALENZUELA C, GIL M, et al.Salmonella enters a dormant state within human epithelial cells for persistent infection[J].PLoS Pathog, 2021, 17(4):e1009550.
[9]	MARUS J R, MAGEE M J, MANIKONDA K, et al.Outbreaks of Salmonella enterica infections linked to animal contact:demographic and outbreak characteristics and comparison to foodborne outbreaks-United States, 2009-2014[J].Zoonoses Public Health, 2019, 66(4):370-376.
[10]	仇汝龙, 范志宇, 薛家宾, 等.一株兔源荚膜血清D型多杀性巴氏杆菌的分离和鉴定[J].中国养兔, 2021(4):9-12.QIU R L, FAN Z Y, XUE J B, et al.Isolation and identification of a rabbit origin Pasteurella Multocida Serogroup D[J].Chinese Journal of Rabbit Farming, 2021(4):9-12.(in Chinese)
[11]	张春姝.獭兔病毒性出血症与大肠杆菌病混合感染的诊治[J].黑龙江畜牧兽医, 2008(5):78.ZHANG C S.Diagnosis and treatment of mixed infection of viral hemorrhagic disease and Escherichia coli in Rex rabbits[J].Heilongjiang Animal Science and Veterinary Medicine, 2008(5):78.(in Chinese)
[12]	亓玉卓, 梁琳, 林伟东, 等.鸽源鼠伤寒沙门氏菌的分离鉴定[J].动物医学进展, 2021, 42(2):122-127.QI Y Z, LIANG L, LIN W D, et al.Isolation and identification of pigeon-origin Salmonella typhimurium[J].Progress in Veterinary Medicine, 2021, 42(2):122-127.(in Chinese)
[13]	查华, 石火英, 吉贞颖, 等.华东地区鸡白痢沙门氏菌毒力基因的分子流行病学调查[J].中国预防兽医学报, 2012, 34(12):996-998.ZHA H, SHI H Y, JI Z Y, et al.Molecular epidemiological survey of Salmonella pullorum isolates with virulence gene in eastern China[J].Chinese Journal of Preventive Veterinary Medicine, 2012, 34(12):996-998.(in Chinese)
[14]	张春玲, 邹勇, 周宗清, 等.两株高致病性猪繁殖与呼吸综合征病毒与猪霍乱沙门氏菌的分离及人工共感染猪的致病性试验[J].中国预防兽医学报, 2013, 35(7):562-565.ZHANG C L, ZOU Y, ZHOU Z Q, et al.Isolation of two highly pathogenic PRRSV coinfected with Salmonella cholersuis and experimental reproduction of the disease in pigs[J].Chinese Journal of Preventive Veterinary Medicine, 2013, 35(7):562-565.(in Chinese)
[15]	WANG H, LIU K J, SUN Y H, et al.Abortion in donkeys associated with Salmonella abortus equi infection[J].Equine Vet J, 2019, 51(6):756-759.
[16]	刘照波, 李明勇, 张彩虹, 等.一例兔沙门氏菌病流行病学调查与诊断分析[J].中国养兔, 2021(1):16-19.LIU Z B, LI M Y, ZHANG C H, et al.An epidemiological investigation and diagnostic analysis of Salmonellosis in rabbits[J].Chinese Journal of Rabbit Farming, 2021(1):16-19.(in Chinese)
[17]	李欣.兔沙门氏菌与大肠杆菌混合感染的诊治[J].河南畜牧兽医, 2006, 27(2):50.LI X.Diagnosis and treatment of rabbit Salmonella and Escherichia coli co-infection[J].Henan Journal of Animal Husbandry and Veterinary Medicine, 2006, 27(2):50.(in Chinese)
[18]	HORLBOG J A, STEPHAN R, STEVENS M J A, et al.Feedborne Salmonella enterica serovar Jerusalem outbreak in different organic poultry flocks in Switzerland and Italy linked to soya expeller[J].Microorganisms, 2021, 9(7):1367.
[19]	CASANOVA-HIGES A, MARÍN-ALCALÁ C M, ANDRÉS-BARRANCO S, et al.Weaned piglets:another factor to be considered for the control of Salmonella infection in breeding pig farms[J].Vet Res, 2019, 50(1):45.
[20]	GALL A J, BURROUGH E R, ZHANG J Q, et al.Identification and correlation of a novel siadenovirus in a flock of budgerigars (Melopsittacus undulates) infected with Salmonella typhimurium in the United States[J].J Zoo Wildl Med, 2020, 51(3):618-630.
[21]	谭烁, 冯帆, 陈极奎, 等.一株山羊源肠炎沙门氏菌对小鼠的致病性研究[J].安徽农业科学, 2018, 46(13):90-94, 143.TAN S, FENG F, CHEN J K, et al.Study on the pathogenicity of a strain of Salmonella enteritidis from goat to mice[J].Journal of Anhui Agricultural Sciences, 2018, 46(13):90-94, 143.(in Chinese)
[22]	刘俊伟, 范国英, 张海棠, 等.猪沙门氏菌病的组织病理学观察[J].贵州农业科学, 2011, 39(6):141-143.LIU J W, FAN G Y, ZHANG H T, et al.Histopathological observation of porcine Salmonellosis[J].Guizhou Agricultural Sciences, 2011, 39(6):141-143.(in Chinese)
[23]	LALSIAMTHARA J, LEE J H.Pathogenic traits of Salmonella Montevideo in experimental infections in vivo and in vitro[J].Sci Rep, 2017, 7:46232.
[24]	陈均华, 梁晋立.兔鼠伤寒沙门菌性流产的诊断[J].中国兽医科技, 2003, 33(6):59-60.CHEN J H, LIANG J L.Diagnosis of abortion resulted from Salmonella typhimurium in domestic rabbits[J].Chinese Veterinary Science, 2003, 33(6):59-60.(in Chinese)
[25]	董文秀.家兔沙门氏菌病的诊疗[J].畜牧兽医科技信息, 2016(6):133.DONG W X.Diagnosis and treatment of rabbit Salmonellosis[J].Chinese Journal of Animal Husbandry and Veterinary Medicine, 2016(6):133.(in Chinese)
[26]	MASUET-AUMATELL C, ATOUGUIA J.Typhoid fever infection-Antibiotic resistance and vaccination strategies:a narrative review[J].Travel Med Infect Dis, 2021, 40:101946.
[27]	SEARS K T, GALEN J E, TENNANT S M.Advances in the development of Salmonella-based vaccine strategies for protection against Salmonellosis in humans[J].J Appl Microbiol, 2021, 131(6):2640-2658.
[28]	MANESH A, MELTZER E, JIN C, et al.Typhoid and paratyphoid fever:a clinical seminar[J].J Travel Med, 2021, 28(3):taab012.
[29]	刘芳萍, 赵玉林, 李昌文, 等.鸡源性沙门氏菌耐药基因检测与耐药相关性分析[J].中国预防兽医学报, 2013, 35(8):627-630.LIU F P, ZHAO Y L, LI C W, et al.Drug resistance gene detection and the resistance correlation analysis in Salmonella isolated from chickens[J].Chinese Journal of Preventive Veterinary Medicine, 2013, 35(8):627-630.(in Chinese)
[30]	李珂, 黄凯伦, 解新迪, 等.七株鹅肠道沙门氏菌的分离鉴定、药敏试验及毒力基因、耐药基因的检测[J].安徽科技学院学报, 2021, 35(5):34-40.LI K, HUANG K L, XIE X D, et al.Isolation and identification of 7 Enteric salmonella strains, drug susceptibility test, virulence gene and drug resistance gene[J].Journal of Anhui Science and Technology University, 2021, 35(5):34-40.(in Chinese)

Pathological Observation and Drug Sensitivity Analysis of Salmonella Infection in Female Rabbits

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