外源MDR鼠伤寒沙门菌对健康小鼠肠道菌群的影响

doi:10.11843/j.issn.0366-6964.2023.05.036

Abstract

Abstract: In this study, we aimed to explore the impact of exogenous multidrug resistant (MDR) Salmonella Typhimurium on gut microbiota of healthy mice. Twenty-five mice were randomly divided into quality control group (C), one day gavage group (G1), three days gavage group (G2), five days gavage group (G3) and seven days gavage group (G4), with 5 mice in each group. Resistant Salmonella Typhimurium from swine carrying drug resistance genes was administered to mice in experimental groups except the quality control group by gavage at a concentration of 10⁶ CFU·mL^-1. Fresh fecal samples were collected on day 0 before gavage and day 1-14 after gavage. High-throughput sequencing technology was used to analyze the diversity of gut microbiota in feces of mice after gavage. The results showed as follows:1) Compared with group C, all experimental groups showed mild diarrhea after gavage, MDR Salmonella Typhimurium was isolated from fecal samples with the same type as gavage bacteria, and there was no significant difference in the isolation rate. 2) The Chao1, Goods_coverage and Observed_species indices in Alpha diversity of gut microbiota in experimental group were significantly higher than those in group C; 3) The relative abundance of Epsilonbacteraeota and Helicobacteraceae at phylum and genus level in groups G2, G3 and G4 was significantly lower than that in group C (P<0.05). According to LEfSe analysis, Epsilonbacteraeota phylum and Helicobacter genus were significantly enriched and had the highest abundance in group C. 4) A total of 10 metabolic pathways were screened out from the gut microbiota of mice in each experimental group and group C. Compared with group C, the glycogen degradation Ⅱ metabolic pathway in G1 group was down-regulated, while the thiol biosynthesis and NAD biosynthesis Ⅱ metabolic pathways in G2, G3 and G4 groups were significantly up-regulated (P<0.05) to regulate gut balance. In conclusion, persistent infection with MDR Salmonella Typhimurium can increase the diversity and richness of intestinal flora in mice. Long-term oral administration of MDR Salmonella Typhimurium can promote the regulation of intestinal flora through metabolic pathways. However, due to the complexity of intestinal flora itself, the self-regulation and related mechanisms of intestinal flora need to be further studied.

Key words: Salmonella Typhimurium, multidrug resistant, gut microbiota, high throughput sequencing

CLC Number:

Q938.1

QIN Lei, WU Huimin, XU Qiqi, CHEN Wanzhao, WANG Dong, LI Hongbo, XIA Panpan, LIU Zepeng, XIA Lining. Effect of Exogenous Drug-Resistant Salmonella Typhimurium on Intestinal Flora in Healthy Mice[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2158-2169.

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Effect of Exogenous Drug-Resistant Salmonella Typhimurium on Intestinal Flora in Healthy Mice

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