Abstract: The aims of this paper were to construct a mouse model of dust mite induced atopic dermatitis (AD) and a mouse model of asthma. In this experiment, the main allergens Der p 1, Der f 1, and Blo t 5 of the three main dust mite species (Dermatophagoides pteronyssinus, Dermatophagoides farinae, and Blomia tropicalis) were mixed in equal ratios to induce an AD mouse model and an asthma mouse model, respectively. The degree of sensitization in AD mice was also assessed by the levels of relevant inflammatory cytokines (IFN-γ, IL-4, IL-10, IL-17A) in skin and lung tissues, and total IgE serum levels. IgE levels, ear prick test, and hematoxylin-eosin staining of lung tissue (HE) were used to assess the asthma mouse model. The results showed that the skin of the sensitized area in the AD model showed crusting after breakage, and the changes of cytokine levels in the skin and lung tissues indicated that the in vivo response of the mice was in favor of Th2 response, and the IgE level was significantly higher than that of the normal group; The total serum IgE level was significantly increased in the mice of the asthma model group. The body temperature of the model mice decreased significantly after allergen excitation and did not recover the initial temperature after 90 min. In the ear prick test, the antigen attack increased the leakage of ear dye in the model mice, and the HE staining of lung tissue shows increased inflammatory cell infiltration in the model group of mice. This experiment successfully induced dust mite AD mouse model and dust mite asthma mouse model.

Key words: atopic dermatitis, asthma, cytokines, animal models