猪产业链中鼠伤寒沙门菌及沙门菌血清型4,[5],12:i:-基因组学分析

doi:10.11843/j.issn.0366-6964.2023.03.031

Abstract

Abstract: Whole genomes of 91 Salmonella isolates from pig-borne food chain of Henan province were sequenced by Illumina NovaSeq 6000 platform for the understanding of genomic characteristics of Salmonella typhimurium and Salmonella serotype 4,[5],12:i:-. Serotype prediction, multi-locus sequence typing, plasmid types, virulence genes and drug resistance genes were analyzed by bioinformatics. The results showed that the prevalence of Salmonella 4,[5],12:i:- (1.58%, 50/3 173) was higher than that of Salmonella typhimurium (1.29%, 41/3 173) in pig-borne food chain of Henan Province. Sequence types(STs) of 91 Salmonella isolates included ST34 (71.42%, 65/91) and ST19 (28.57%, 26/91). Typhimurium/ST34 strains and 4,[5],12:i:-/ST34 strains were the preferred hosts of some plasmids including IncHI2A _1, IncHI2 _1 and Col440I _1. Resistant genes highly carried by Salmonella serotypes 4,[5],12:i:- were blaTEM-1B_1 (72%), tet (B)_2 (90%), sul2_3 (66%), blaOXA-1_1 (34%), etc. Compared with Salmonella typhimurium isolates, Salmonella serotype 4,[5],12:i:- isolates had a higher plasmid-carrying rate, carrying a greater variety of resistance genes and virulence genes. This study confirmed for the first time that the prevalence of Salmonella serotype 4,[5],12:i:- has exceeded that of Salmonella typhimurium in pig-borne food chain of Henan Province. To a certain extent, this may explain the predominant prevalence of the Salmonella serotype among patients in the area.

Key words: Salmonella serotype 4,[5],12:i-, Salmonella typhimurium monophasic variant, pig-borne food chain, genomics, drug-resistant genes, virulence genes

CLC Number:

S852.61

JIANG Zenghai, TENG Lin, HE Anwen, LIU Yanyan, YUE Min, HE Qigai. Genomic Analysis of Salmonella Typhimurium Isolates and Salmonella Serotype 4, [5], 12: i:- Isolates from Pig-borne Food Chain[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1199-1209.

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Genomic Analysis of Salmonella Typhimurium Isolates and Salmonella Serotype 4, [5], 12: i:- Isolates from Pig-borne Food Chain

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