沙门菌SptP调控巨噬细胞MAPK-CDK6-RB通路的初步分析

doi:10.11843/j.issn.0366-6964.2023.03.030

Abstract

Abstract: The purpose of this study was to explore the effect of Salmonella virulence factor SptP on MAPK-CDK6-RB pathway and the formation of macrophage extracellular traps (METs). Firstly, compare the difference of METs levels induced by Salmonella 13312 (Salmonella Choleraesuis), Salmonella 14028 (Salmonella Typhimurium) and Salmonella 13314 (Salmonella Arizona). Then, predict the Salmonella genes involved in the regulation of METs using bioinformatics method. Finally, verify the result using ChIP and MALDI-TOF experiments. The results showed that the levels of METs induced by Salmonella 13312 and Salmonella 14028 were significantly lower than Salmonella 13314 (P<0.05), suggested that Salmonella genes may regulate some key steps of the biogenesis of METs through a certain pathway. The bioinformatics prediction of Domain—Domain interaction combined with ChIP and MALDI-TOF experiments showed that the Salmonella gene SptP participated in this process, and its host target molecules may be the downstream genes, ABL1 and CDK6, of MAPK pathway. SptP may participate in the regulation of MAPK-CDK6-RB pathway by regulating the dephosphorylation of ABL1 and CDK6, this pathway may responsible for the biogenesis of METs. The results suggest that SptP-MAPK-CDK6-RB pathway may be an important pathway for Salmonella to escape the host immune system. The discovery of this pathway provides new insights for the study of the mechanism of infection and long-term carrier of Salmonella to escape the host immune system.

Key words: Salmonella, SptP, extracellular traps, macrophages, MAPK, ABL1

CLC Number:

S855.1

YAO Min, SHI Bomei, HUANG Tinghua. A Preliminary Research of the Regulation of MAPK-CDK6-RB Pathway by Salmonella SptP in Macrophages[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 1187-1198.

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A Preliminary Research of the Regulation of MAPK-CDK6-RB Pathway by Salmonella SptP in Macrophages

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