马立克病病毒超强毒株GX0101感染宿主部分病毒基因表达水平及致病阶段分析

doi:doi: 10.11843/j.issn.0366-6964.2016.08.014

Abstract

Abstract:

The present work was performed to study the infection phases of Marek’s disease (MD)，namely ‘Cornell Model’，caused by the very virulent (vv) Marek’s disease virus (MDV) strain GX0101.For the first step，one-day-old specific pathogen free (SPF) chickens were challenged by GX0101 to establish the animal model of MD.Then，the kinetic transrption levels of 10 representative MDV protein-coding genes at different days post-infection (dpi) were determined by real-time quantitative PCR (RT-qPCR).The results showed that the onset of clinical symptoms and autopsy lesions of GX0101-infected birds was consistent with the typical MD cases.Some of the MDV protein-coding genes，including the structural protein-coding genes of gB，gE and UL6，the nonstructural protein-coding genes of UL42 and UL52，the immediate early infected cell protein 4 gene (ICP4)，the horizontal transmission associated gene of UL13 and the 38 kD phosphorylated protein gene (pp38)，demonstrated a similar transcription pattern，which increased to a first peak at 7 dpi，fell to a bottom level at 10 dpi，increased again at 14 dpi and reached to a second peak at 21 dpi，and then gradually declined to low levels from 30 to 60 dpi.However，both of the unique MDV oncogene meq and virulence related gene RLORF6 represented a persistent increasing transcription trend from 7 dpi，and then kept at a higher transcription level from 14 to 60 dpi.Our previous and present data indicates that the ‘Cornell Model’ of MD caused by GX0101 are as blow：the early cytolytic phase occurs at 1-7 dpi，the latent phase establishes at about 10 dpi and the late cytolytic and immunosuppressive phase appears at 14-21 dpi，while the T cell transformation and lymphomagenesis possibly starts at about 14 dpi.

CLC Number:

S852.659.1

MA Sheng-ming，TENG Man，YU Zu-hua，DANG Lu，LI Hui-zhen，DING Ke，DENG Rui-guang，LUO Jun. The Viral Gene Expression Profiles and Pathogenic Phases of the Disease Caused by Very Virulent MDV Strain GX0101[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(8): 1635-1644.

0
/ / Recommend

Add to citation manager EndNote|Reference Manager|ProCite|BibTeX|RefWorks

URL: https://www.xmsyxb.com/EN/doi: 10.11843/j.issn.0366-6964.2016.08.014

https://www.xmsyxb.com/EN/Y2016/V47/I8/1635

References

［1］SAIF Y M.禽病学［M］.苏敬良，高福，索勋，译.12版.北京：中国农业出版社，2012：129-151.
SAIF Y M.Avain disease［M］.SU J L，GAO F，SUO X，translated.12ed.Beijing：China Agriculture Press，2012：129-151.(in Chinese)
［2］DAVISON A J，EBERLE R，EHLERS B，et al.The order Herpesvirales［J］.Arch Virol，2009，154(1)：171-177.
［3］WITTER R L.Increased virulence of Marek’s disease virus field isolates［J］.Avian Dis，1997，41(1)：149-163.
［4］TENG L Q，WEI P，SONG Z B，et al.Molecular epidemiological investigation of Marek’s disease virus from Guangxi，China［J］.Arch Virol，2011，156(2)：203-206.
［5］TIAN M，ZHAO Y，LIN Y，et al.Comparative analysis of oncogenic genes revealed unique evolutionary features of field Marek’s disease virus prevalent in recent years in China［J］.Virol J，2011，8：121.
［6］ZHANG Y P，LIU C J，ZHANG F，et al.Sequence analysis of the Meq gene in the predominant Marek’s disease virus strains isolated in China during 2006-2008［J］.Virus Genes，2011，43(3)：353-357.
［7］YU Z H，TENG M，LUO J，et al.Molecular characteristics and evolutionary analysis of field Marek’s disease virus prevalent in vaccinated chicken flocks in recent years in China［J］.Virus Genes，2013，47(2)：282-291.
［8］ZHANG Y P，LV H C，BAO K Y，et al.Molecular and pathogenicity characterization of Gallid herpesvirus 2 newly isolated in China from 2009 to 2013［J］.Virus Genes，2016，52(1)：51-60.
［9］CUI N，SU S，SUN P，et al.Isolation and pathogenic analysis of virulent Marek’s disease virus field strain in China［J］.Poult Sci，2016，pii：pew073.
［10］CALNEK B W.Pathogenesis of Marek’s disease virus infection［J］.Curr Top Microbiol Immunol，2001，255：25-55.
［11］BAIGENT S J，DAVISON F，NAIR V.Marek’s disease virus：biology and life cycle，in Marek’s Disease［M］.An Evolving Problem.Elsevier Academic Press，Oxford，United Kingdom，2004：62-77.
［12］HU X，QIN A，XU W，et al.Transcriptional analysis of host responses to Marek’s disease virus infection in chicken thymus［J］.Intervirology，2015，58(2)：95-105.
［13］ZHANG Z，CUI Z.Isolation of recombinant field strains of Marek’s disease virus integrated with reticuloendotheliosis virus genome fragments［J］.Sci China C Life Sci，2005，48(1)：81-88.
［14］CUI Z，ZHUANG G，XU X，et al.Molecular and biological characterization of a Marek’s disease virus field strain with reticuloendotheliosis virus LTR insert［J］.Virus Genes，2010，40(2)：236-243.
［15］SUN A J，LAWRENCE P，ZHAO Y G，et al.A BAC clone of MDV strain GX0101 with REV-LTR integration retained its pathogenicity［J］.Chin Sci Bull，2009，54(15)：2641-2647.
［16］SUN A J，XU X Y，PETHERBRIDGE L，et al.Functional evaluation of the role of reticuloendotheliosis virus long terminal repeat (LTR) integrated into the genome of a field strain of Marek’s disease virus［J］.Virology，2010，397(2)：270-276.
［17］SU S，CUI N，SUN A，et al.Sequence analysis of the whole genome of a recombinant Marek’s disease virus strain，GX0101，with a reticuloendotheliosis virus LTR insert［J］.Arch Virol，2013，158(9)：2007-2014.
［18］SU S，CUI N，CUI Z，et al.Complete genome sequence of a recombinant Marek’s disease virus field strain with one reticuloendotheliosis virus long terminal repeat insert［J］.J Virol，2012，86(24)：13818-13819.
［19］LUO J，SUN A J，TENG M，et al.Expression profiles of microRNAs encoded by the oncogenic Marek’s disease virus reveal two distinct expression patterns in vivo during different phases of disease［J］.J Gen Virol，2011，92(Pt 3)：608-620.
［20］YU Z H，TENG M，SUN A J，et al.Virus-encoded miR-155 ortholog is an important potential regulator but not essential for the development of lymphomas induced by very virulent Marek’s disease virus［J］.Virology，2014，448，55-64.
［21］TENG M，YU Z H，SUN A J，et al.The significance of the individual Meq-clustered miRNAs of Marek’s disease virus in oncogenesis［J］.J Gen Virol，2015，96(Pt 3)：637-649.
［22］ZHAO P，LI X J，TENG M，et al.In vivo expression patterns of microRNAs of Gallid herpesvirus 2 (GaHV-2) during the virus life cycle and development of Marek’s disease lymphomas［J］.Virus Genes，2015，50(2)：245-252.
［23］CHI J Q，TENG M，YU Z H，et al.Marek’s disease virus-encoded analog of microRNA-155 activates the oncogene c-Myc by targeting LTBP1 and suppressing the TGF-β signaling pathway［J］.Virology，2015，476：72-84.
［24］CHI X J，LU Y X，ZHAO P，et al.Interaction domain of glycoproteins gB and gH of Marek’s disease virus and identification of an antiviral peptide with dual functions［J］.PLoS One，2013，8(2)：e54761.
［25］SCHUMACHER D，TISCHER B K，REDDY S M，et al.Glycoproteins E and I of Marek’s disease virus serotype 1 are essential for virus growth in cultured cells［J］.J Virol，2001，75(23)：11307-11318.
［26］LZUMIYA Y，JANG H K，CAI J S，et al.Identification and sequence analysis of the Marek’s disease virus serotype 2 gene homologous to the herpes simplex virus type 1 UL52 protein［J］.J Vet Med Sci，1999，61(6)：683-687.
［27］KATO K，LZUMIYA Y，TOHYA Y，et al.Identification and characterization of Marek’s disease virus serotype 1 (MDV1) ICP22 gene product：MDV1 ICP22 transactivates the MDV1 ICP27 promoter synergistically with MDV1 ICP4［J］.Vet Microbiol，2002，85(4)：305-313.
［28］JAROSINSKI K W，OSTERRIEDER N.Further analysis of Marek’s disease virus horizontal transmission confirms that U(L)44 (gC) and U(L)13 protein kinase activity are essential，while U(S)2 is nonessential［J］.J Virol，2010，84(15)：7911-7916.
［29］GIMENO I M，WITTER R L，HUNT H D，et al.The pp38 gene of Marek’s disease virus (MDV) is necessary for cytolytic infection of B cells and maintenance of the transformed state but not for cytolytic infection of the feather follicle epithelium and horizontal spread of MDV［J］.J Virol，2005，79(7)：4545-4549.
［30］LI Y，SUN S，SU S，et al.Deletion of the Meq gene significantly decreases immunosuppression in chickens caused by pathogenic Marek’s disease virus［J］.Virol J，2011，8：2.
［31］SPATZ S J，SILVA R F.Polymorphisms in the repeat long regions of oncogenic and attenuated pathotypes of Marek’s disease virus 1［J］.Virus Genes，2007，35(1)：41-53.
［32］崔治中.禽反转录病毒与DNA病毒间的基因重组及其流行病学意义［J］.病毒学报，2006，22(2)：150-154.
CUI Z Z.The epidemiological significance of the genetic recombination between avian retroviruses and DNA virus［J］.Chinese Journal of Virology，2006，22(2)：150-154.(in Chinese)

[1]	CHI Lili, WANG Junna, ZHANG Yuming, LIU Jian, CHEN Zhiyuan, LI Shufan, YIN Yanbo, XU Shouzhen. Prokaryotic Expression and Immunogenicity Analysis of Truncated Fusion Protein of FAdV-4 Fiber2 and FAdV-8b Fiber [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5162-5170.
[2]	LI Linyan, TENG Man, LIU Jinling, ZHENG Luping, CHAI Shujun, DING Ke, YU Zuhua, LUO Jun. Development of a Monoclonal Antibody Pool for Marek’s Disease Virus and Identification of a Specific mAb against the Glycoprotein gB [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4712-4723.
[3]	ZHANG Fangyuan, YANG Dawei, QIU Deyang, JIANG Guoqian, LI Guimei, SHAN Hu. Expression of ASFV P30 Protein and Development of ASFV Antibody Detection Method Based on x-MAP Technology [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4300-4310.
[4]	FENG Yongzhi, GONG Ting, WU Dongdong, GAO Qi, ZHENG Xiaoyu, ZHANG Guihong, SUN Yankuo. Analysis of Factors Affecting the Infectivity of African Swine Fever Virus on Cultured Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3406-3414.
[5]	ZHANG Yi, WANG Chenyan, YANG Xiao, SHAO Guoqing, HOU Bo. Phylogenetic Analysis and Pathogenicity of an Avian Infectious Laryngotracheitis Virus Strain WF03 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3435-3444.
[6]	DING Xiaoyan, HE Jiuxiang, ZHOU Xiaoyang, ZHOU Yuxin, LI Jintao. Preliminary Identification of Host Regulatory Genes and Virulence Genes during African Swine Fever Virus Infection [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2964-2971.
[7]	CHEN Hongjian, CAO Yan, FAN Jie, GAN Rongxuan, SONG Wenbo, YU Shengwei, YANG Ting, ZHAO Yanxia, WEI Chunyan, XIE Rui, HUA Lin, PENG Zhong, WU Bin. Prevalence and Phylogenetic Analysis of Pseudorabies Virus within Pig Slaughterhouses in Hubei Province of China during 2020-2022 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2972-2981.
[8]	WANG Jiali, ZHOU Ning, CHEN Xi, YUE Hua, TANG Cheng. Isolation, Identification and Pathogenicity of Canine Adenovirus Type 2 Strain with Natural Deletion in E3 Gene [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2982-2990.
[9]	FENG Weimin, LIU Xiao, HUANG Teng. The Evasion Strategy against CTL Recognition by Herpesviruses of Domestic Animals: Interference with MHC Class Ⅰ Antigen Presentation Pathway [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2241-2251.
[10]	GAO Zhenzhen, MENG Zejing, HE Xiaobing, FANG Yongxiang, TIAN Huihui, CHEN Guohua, JING Zhizhong. Identification of Immunogenicity and Neutralizing Activity of Ectromelia Virus EVM135 and EVM085 Proteins [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2468-2477.
[11]	WANG Ying, ZHU Jiahong, ZHAO Jiakai, JI Pinpin, CHEN Xu, ZHANG Lu, LIU Baoyuan, SUN Yani, ZHAO Qin. Screening and Identification of Nanobodies against NP419L Protein of African Swine Fever Virus and Its Preliminary Application of Antibody Detection [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2509-2520.
[12]	QIU Yingwu, CHANG Hao, PENG Jie, YI Heyou, WANG Qiumei, GUO Yanchen, WU Qianwen, CAO Xuezhen, LIN Limiao, LI Wei, ZHOU Qingfeng, ZHANG Guihong, LI Qunhui, GONG Lang. A Real-time PCR Method for the Rapid Detection of African Swine Fever Virus I177L Gene Deletion Strain [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2521-2527.
[13]	LONG Qinqin, WEI Min, WANG Yuting, WEN Ming, PANG Feng. The Battle between Orf Virus and Host: Immune Response and Viral Immune Evasion Mechanisms [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1845-1853.
[14]	WANG Guochao, ZHAO Yaru, ZHANG Zhonghui, ZHANG Yulong, BAI Ge, GENG Shuxian, FAN Jie, YANG Jifei, GUAN Guiquan, YIN Hong, LUO Jianxun, NIU Qingli. Bioinformatics Analysis of RNA Polymerase Subunit D205R Gene of African Swine Fever Virus and Polyclonal Antibody Preparation [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2042-2049.
[15]	YUAN Sheng, LI Anqi, Lü Wenke, YANG Lulu, ZHOU Feng, HUANG Liangzong, BAI Aiquan, WEN Feng, HUANG Shujian, GUO Jinyue. Analysis of Variation in Major Virulence-related Genes of a Strain of Pseudorabies Virus and Its Pathogenicity to Rabbits [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2195-2199.

The Viral Gene Expression Profiles and Pathogenic Phases of the Disease Caused by Very Virulent MDV Strain GX0101

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments