贵州省鸭源新城疫病毒强毒株的遗传变异分析及致病性研究

doi:doi: 10.11843/j.issn.0366-6964.2016.08.013

Abstract

Abstract:

To better evaluate the role of domestic ducks in the epidemiology and transmission of NDV，five virulent NDV strains isolated from ducks in Guizhou Province were used to study their genetic variation and pathogenicity in ducks and SPF chickens.Sequence analysis showed that all the strains belonged to subgenotype Ⅶd NDV and had the velogenic motif 112RRQKRF117 in the cleavage sites of F protein，which was consistent with the results of biological tests.The functional amino acid sites in the F and HN proteins were all highly conserved，but three strains with E347K mutation were detected in the linear epitope of HN protein.Cross hemagglutination inhibition test revealed that the antigen homology of the isolates between LaSota and A-Ⅶ were 83.3%-87.0% and 93.5%-100%，respectively.In the challenge test，ducks of different groups challenged with virus allantoic fluid intramuscularly at the dose of 0.5 mL showed no obvious clinical symptoms and histopathologic changes，and viruses could not be detected in multiple tissues except for the spleen.On the contrary，SPF chickens infected with NDV strains intranasally at a dose of 10 ^6.0 ELD50•0.1 mL^－1 died in 6 days and showed typical clinical symptoms.Viruses could replicate in multiple tissues and cause severe damages to spleen，thymus and bursa in chickens.In addition，viruses were detected more frequently in the laryngeal and cloacal swabs of chickens than ducks.These results showed that subgenotype Ⅶd NDV strains with E347K mutation on HN protein significantly increased and had the antigenic differences with traditional vaccine strain.Although all the NDV strains were virulent in chickens whereas had no obvious pathogenicity in ducks，some measures should be taken to prevent virus transmission from ducks to chickens as viruses were detected in swabs of virulent NDV infected ducks for a long time.

CLC Number:

DUAN Zhi-qiang，XU Hou-qiang，JI Xin-qin，CHEN Qiang，HU Yan，HU Shun-lin，LIU Xiu-fan. Genetic Variation and Pathogenicity Analysis of Virulent Newcastle Disease Viruses Isolated from Ducks in Guizhou Province[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, doi: doi: 10.11843/j.issn.0366-6964.2016.08.013 .

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Genetic Variation and Pathogenicity Analysis of Virulent Newcastle Disease Viruses Isolated from Ducks in Guizhou Province

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