新城疫病毒F蛋白纳米抗体的筛选及活性鉴定

doi:doi: 10.11843/j.issn.0366-6964.2016.08.015

Abstract

Abstract:

This study aimed to obtain VHH (variable domains of camelid heavy-chain antibodies，VHH) against Newcastle disease virus (NDV) fusion protein (F protein).The synthesised DNA sequence encoding neutralizing epitope of F protein was used to construct bait plasmid.Then，the constructed bait was used to screen a naive Camelus Bactrianus VHH yeast two-hybrid library through yeast two-hybrid technology.After screening，four positive clones were primarily obtained and two of four VHHs with hallmark of VHH sequence were picked out for expression and purification.Subsequently，the biological activities of purified VHH were evaluated.Indirect ELISA results showed that the reactivity between VHH1 or VHH2 and NDV virion was significant higher than negative control.Western blot results suggested that VHH could specifically react with F protein.Neutralization assay further demonstrated that these two VHH could inhibit the replication of virulent NDV at some extent.These results indicate that we successfully obtained two VHH against NDV F protein，which laid the foundation for its application in ND control.

CLC Number:

GAO Xiao-long，HU Xiang-yun，FU Xiang-jing，WANG Yan-ping，TONG Li-na，WANG Hai-xin，WANG Xing-long，ZHANG Shu-xia，XIAO Sa，DU En-qi，YANG Zeng-qi. Screening and Characterization of VHH against Newcastle Disease Virus Fusion Protein[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2016, 47(8): 1645-1651.

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Screening and Characterization of VHH against Newcastle Disease Virus Fusion Protein

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