H9N2亚型禽流感病毒血凝素蛋白分子特征分析及其对小鼠致病力的研究

doi:10.11843/j.issn.0366-6964.2014.03.014

Abstract

Abstract:

The study was conducted to understand the evolution of hemagglutinin of H9N2 subtype avian influenza viruses (AIV) isolated from Shandong province and evaluate their pathogenicity to mammals.We analyzed hemagglutinin amino acids sequences of 5 H9N2 AIV isolated from Shandong province，and assessed their pathogenicity to mammals by using BABL/c mice model.The results indicated that all 5 strains belonged to CK/Beijing lineage.Two strains of them possessed human-like receptor binding specificity (Leu²³⁴)，while other three possessed avian-like receptor binding specificity (Gln²³⁴).The HA cleavage site showed that all the strains were low pathogenic.Potential glycosylation sites varied among the viruses.Two strains could replicate in mice’s lung，caused interstitial pneumonia，and could be detected in bronchial epithelial cells by immunohistochemistry.Our study demonstrated that the HA gene and its coding protein had some difference during the evolution of H9N2 subtype AIV，and two of the researched strains could infect mammal.This study provided theoretical basis at molecular level for further studies on amino acids of H9N2 subtype AIV HA protein，which relate to cross-species transmission.

CLC Number:

S852.659.5

HUANG Rong， LIANG Wei， LIU Ji-yuan， WANG Ai-rong， CHAI Tong-jie. Analysis of Hemagglutinin Molecular Characterization of H9N2 Avian Influenza Virus and Study on Its Pathogenicity to Mice[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(3): 443-450.

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Analysis of Hemagglutinin Molecular Characterization of H9N2 Avian Influenza Virus and Study on Its Pathogenicity to Mice

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