牦牛源牛呼吸道合胞体病毒的分子流行病学调查

doi:10.11843/j.issn.0366-6964.2023.05.024

Abstract

Abstract: Bovine respiratory syncytial virus (BRSV) is an important pathogen of bovine respiratory disease complex (BRDC). This experiment aims to investigate whether BRSV were present in Tibetan plateau yaks and its molecular prevalence in yaks with respiratory diseases. One hundred and twenty-two nasal swabs from Ganzi (Garzê) Tibetan Autonomous Prefecture and Aba (Ngawa) Tibetan and Qiang Autonomous Prefecture were collected from Yak with BRDC in 10 farms across Sichuan provinces from March 2021 to July 2022, among them, 91 samples were from Ganzi Prefecture and 31 samples were from Aba Prefecture. Using reverse transcription insulated isothermal PCR (RT-iiPCR), 64.75% of samples tested positive for BRSV. Among them, the positive rate in Ganzi Prefecture and Aba Prefecture was 73.63% and 38.71% respectively, and the farm positive rate was 100%. Further, 10 complete G genes were identified as subgroup Ⅲ strains, and 9 complete F genes were amplified from positive samples. Compared to known BRSV strains in GenBank, G proteins and F proteins from yaks with BRSV strains previously amplified by our laboratory shared several identical amino acid mutations. Moreover, complete genomes from Yaks were obtained, which was closest to the BRSV strain (GenBank accession number:OP137030-OP137034) uploaded recently by our laboratory. In conclusion, this study confirmed the existence and prevalence of BRSV in yaks for the first time, obtained a genome sequence of BRSV in the Ⅲ subgroup from yaks, enriched the pathogenic spectrum of respiratory diseases in yaks, and provided a reference for the prevention and control of respiratory diseases in yaks.

Key words: yaks, bovine respiratory syncytial virus, molecular epidemiology, genetic characteristics

CLC Number:

CHANG Yiming, TANG Cheng, YUE Hua. Molecular Epidemiological Investigation of Bovine Respiratory Syncytial Virus in Yaks[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2030-2041.

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Molecular Epidemiological Investigation of Bovine Respiratory Syncytial Virus in Yaks

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