B亚型禽偏肺病毒对黄羽肉鸡致病性分析及其灭活疫苗免疫效果评价

doi:10.11843/j.issn.0366-6964.2023.12.025

Abstract

Abstract: To evaluate the protective efficacy of the inactivated vaccine against avian metapneumovirus (aMPV) subtype B in yellow-feathered broilers, the inactivated vaccine LN16-I strain prepared in this study was immunized by intramuscular injection at a dose of 0.5 mL per chicken to yellow-feathered broilers at the age of 3 weeks. Three weeks after immunization, the same injection method and dose were used once to reinforce the immunization. Serum was collected within 1-6 weeks and ELISA antibody and neutralizing antibody titers were measured. The results showed that the average ELISA antibody titer in the vaccinated group can reach 4.2×10⁴ post-priming immunization, the average neutralizing antibody titer reached 7.42 log2, and the positive rate was 100%. Three weeks after the booster immunization, the chickens in each group were challenged with virulent virus LN16 at a dose of 5 000 TCID₅₀ per chicken. The results showed that the chickens in the challenge control group had clinical symptoms such as cloudy or viscous nose, head shaking, and the incidence rate was 85% (11/13), while none of the chickens in the vaccinated group showed clinical symptoms. The number of virus copies in nasal swabs was further detected by RT-qPCR. The result showed that the control group chickens exhibited virus shedding, which reached a maximum at 3 days post-challenge, and the number of virus copies was 4.9×10⁶ copies·mL^-1, while the amount of virus shedding in the vaccinated group decreased by 99.43% compared with that in the control group. The pathological results showed that the turbinate and trachea of the chickens in the control group showed obvious pathological damage, while no obvious pathological changes were found in the vaccinated group. The results of this study show for the first time that the infection with aMPV subtype B can cause obvious disease in yellow-feathered broilers, and the inactivated vaccine LN16-I strain has a good immune protection effect on yellow-feathered broilers. This study provides theoretical support and technical guidance for the epidemiological investigation and effective prevention of aMPV subtype B in yellow-feathered broilers in China.

Key words: avian metapneumovirus subtype B, inactivated vaccine, yellow-feathered broiler, pathogenicity, protective efficacy

CLC Number:

S852.659.5

MENG Lingzhai, CHEN Chunli, YU Mengmeng, WANG Zhanxin, WANG Suyan, LIU Peng, HE Tana, GUO Ru, CHEN Yuntong, LIU Changjun, QI Xiaole, WU Zhiqiang, GAO Yulong. Pathogenicity of Avian Metapneumovirus Subtype B on Yellow Feather Broilers and Evaluation on Immune Effect of Inactivated Vaccine[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 5154-5161.

References

[1] HOU L, HU X H, GUO J S, et al. Avian Metapneumovirus subgroup C induces mitochondrial antiviral signaling protein degradation through the ubiquitin-proteasome pathway[J]. Viruses, 2021, 13(10):1990.
[2] WANG D D, HOU L, ZHU N, et al. Interaction of Nucleolin with the fusion protein of avian Metapneumovirus subgroup C contributes to viral replication[J]. Viruses, 2022, 14(7):1402.
[3] WANG S C, JIANG N, JIANG L J, et al. Establishment and application of a quadruple real-time RT-PCR for detecting avian metapneumovirus[J]. PLoS One, 2022, 17(6):e0270708.
[4] SUN S K, CHEN F, CAO S, et al. Isolation and characterization of a subtype C avian metapneumovirus circulating in Muscovy ducks in China[J]. Vet Res, 2014, 45(1):74.
[5] BALL C, FORRESTER A, HERRMANN A, et al. Comparative protective immunity provided by live vaccines of Newcastle disease virus or avian metapneumovirus when co-administered alongside classical and variant strains of infectious bronchitis virus in day-old broiler chicks[J]. Vaccine, 2019, 37(52):7566-7575.
[6] TEGEGNE D, DENEKE Y, SORI T, et al. Molecular epidemiology and genotyping of infectious bronchitis virus and avian Metapneumovirus in backyard and commercial chickens in Jimma zone, southwestern Ethiopia[J]. Vet Sci, 2020, 7(4):187.
[7] YUN B L, ZHANG Y, LIU Y Z, et al. TMPRSS12 is an activating protease for subtype B avian metapneumovirus[J]. J Virol, 2016, 90(24):11231-11246.
[8] SUN J, WEI Y W, RAUF A, et al. Methyltransferase-defective avian metapneumovirus vaccines provide complete protection against challenge with the homologous Colorado strain and the heterologous Minnesota strain[J]. J Virol, 2014, 88(21):12348-12363.
[9] 沈瑞忠, 曲立新, 于康震, 等. 禽肺病毒的分离鉴定[J]. 中国预防兽医学报, 1999, 21(1):76-77. SHEN R Z, QU L X, YU K Z, et al. Isolation and characterization of an avian pneumovirus from chickens in China[J]. Chinese Journal of Preventive Veterinary Medicine, 1999, 21(1):76-77. (in Chinese)
[10] 郭龙宗, 曲立新. 禽肺病毒对种鸡感染的血清学调查[J]. 家禽科学, 2009(2):33-35.GUO L Z, QU L X. Serologic investigation of avian pneumovirus infection in breeder chickens[J]. Poultry Science, 2009(2):33-35. (in Chinese)
[11] WANG J, HOU L, WEI L, et al. Characterization of avain metapneumovirus subgroup C isolated from chickens in Beijing, China[J]. Poult Sci, 2023, 102(1):102250.
[12] 陈秀琴, 郑敏, 黄梅清, 等. 福建省规模化鸡场禽偏肺病毒感染情况的血清学调查[J]. 动物医学进展, 2021, 42(1):130-133.CHEN X Q, ZHENG M, HUANG M Q, et al. Serosurvey of avian metapneumovirus in scaled chicken farms of Fujian[J]. Progress in Veterinary Medicine, 2021, 42(1):130-133. (in Chinese)
[13] WEI L, ZHU S S, YAN X, et al. Avian metapneumovirus subgroup C infection in chickens, China[J]. Emerg Infect Dis, 2013, 19(7):1092-1094.
[14] 肖志宇, 王素春, 赵成龙, 等. 2020年我国部分地区禽偏肺病毒流行病学调查[J]. 中国预防兽医学报, 2022, 44(7):712-717. XIAO Z Y, WANG S C, ZHAO C L, et al. Epidemiological investigation of avian metapneumovirus in some areas of China in 2020[J]. Chinese Journal of Preventive Veterinary Medicine, 2022, 44(7):712-717. (in Chinese)
[15] YU M M, XING L X, CHANG F F, et al. Genomic sequence and pathogenicity of the first avian metapneumovirus subtype B isolated from chicken in China[J]. Vet Microbiol, 2019, 228:32-38.
[16] SALLES G B C, PILATI G V T, MUNIZ E C, et al. Trends and challenges in the surveillance and control of avian metapneumovirus[J]. Viruses, 2023, 15(9):1960.
[17] BALL C, MANSWR B, HERRMANN A, et al. Avian metapneumovirus subtype B vaccination in commercial broiler chicks:heterologous protection and selected host transcription responses to subtype A or B challenge[J]. Avian Pathol, 2022, 51(2):181-196.
[18] PATNAYAK D P, SHEIKH A M, GULATI B R, et al. Experimental and field evaluation of a live vaccine against avian pneumovirus[J]. Avian Pathol, 2002, 31(4):377-382.
[19] 包媛玲, 宋守生, 于蒙蒙, 等. B亚型禽偏肺病毒病灭活疫苗对商品蛋鸡的免疫效果研究[J]. 中国预防兽医学报, 2022, 44(5):521-525.BAO Y L, SONG S S, YU M M, et al. Study on immune effect of inactivated vaccine of avian metapneumovirus (Subtype B) disease on commercial layer chickens[J]. Chinese Journal of Preventive Veterinary Medicine, 2022, 44(5):521-525. (in Chinese)
[20] BAO Y L, YU M M, LIU P, et al. Novel inactivated subtype B avian Metapneumovirus vaccine induced humoral and cellular immune responses[J]. Vaccines (Basel), 2020, 8(4):762.
[21] WEI L, ZHU S S, SHE R P, et al. Viral replication and lung lesions in BALB/c mice experimentally inoculated with avian metapneumovirus subgroup C isolated from chickens[J]. PLoS One, 2014, 9(3):e92136.
[22] 包媛玲, 何锡栋, 于蒙蒙, 等. B亚型禽偏肺病毒对蛋鸡的致病性研究[J]. 中国家禽, 2021, 43(6):25-30. BAO Y L, HE X D, YU M M, et al. Pathogenicity of avian metapneumovirus subtype B on layer chicken[J]. China Poultry, 2021, 43(6):25-30. (in Chinese)
[23] 冯笑艳, 包媛玲, 于蒙蒙, 等. B亚型禽偏肺病毒对商品肉鸡的致病性研究[J]. 中国预防兽医学报, 2022, 44(10):1034-1038. FENG X Y, BAO Y L, YU M M, et al. Study on pathogenicity of B subtype avian metapneumovirus to commercial broilers[J]. Chinese Journal of Preventive Veterinary Medicine, 2022, 44(10):1034-1038. (in Chinese)
[24] 于蒙蒙, 包媛玲, 王素艳, 等. B亚型禽偏肺病毒的分离鉴定及致病性研究[J]. 畜牧兽医学报, 2022, 53(10):3540-3549. YU M M, BAO Y L, WANG S Y, et al. Isolation, identification and pathogenicity of subtype B avian metapneumovirus[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(10):3540-3549. (in Chinese)
[25] 包媛玲, 何锡栋, 于蒙蒙, 等. B亚型禽偏肺病毒灭活疫苗对商品肉鸡免疫效果的研究[J]. 中国预防兽医学报, 2022, 44(10):1071-1075. BAO Y L, HE X D, YU M M, et al. Study on immune effect of inactivated vaccine of avian metapneumovirus (subtype B) on commercial broiler chickens[J]. Chinese Journal of Preventive Veterinary Medicine, 2022, 44(10):1071-1075. (in Chinese)
[26] PAUDEL S, EASWARAN M, JANG H, et al. Immunization with avian metapneumovirus harboring chicken Fc induces higher immune responses[J]. Virus Res, 2016, 220:129-135.
[27] YOUN H N, NOH J Y, KIM M S, et al. Efficacy of a novel avian metapneumovirus live vaccine candidate based on vaccination route and age[J]. Poult Sci, 2021, 100(3):100528.
[28] SHIRVANI E, VARGHESE B P, PALDURAI A, et al. A recombinant avian paramyxovirus serotype 3 expressing the hemagglutinin protein protects chickens against H5N1 highly pathogenic avian influenza virus challenge[J]. Sci Rep, 2020, 10(1):2221.
[29] KHALID Z, HE L, YU Q, et al. Enhanced protection by recombinant Newcastle disease virus expressing infectious bronchitis virus spike ectodomain and chicken granulocyte-macrophage colony-stimulating factor[J]. Avian Dis, 2021, 65(3):364-372.

Pathogenicity of Avian Metapneumovirus Subtype B on Yellow Feather Broilers and Evaluation on Immune Effect of Inactivated Vaccine

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	CHEN Yuetong, LIU Xiaohan, WANG Zhiyang, ZHAO Yuxin, ZHOU Tiezhong, HU Zengjin, ZHU Yue, WANG Shaohui, TIAN Mingxing, DING Siyu, QI Jingjing, YU Shengqing. Isolation, Identification, Pathogenicity and Drug Susceptibility of Mycoplasma gallisepticum from Dead Chicken Embryos in Large-scale Chicken Farms in Guangdong Province [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 290-299.
[2]	ZHANG Yi, WANG Chenyan, YANG Xiao, SHAO Guoqing, HOU Bo. Phylogenetic Analysis and Pathogenicity of an Avian Infectious Laryngotracheitis Virus Strain WF03 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3435-3444.
[3]	WANG Jiali, ZHOU Ning, CHEN Xi, YUE Hua, TANG Cheng. Isolation, Identification and Pathogenicity of Canine Adenovirus Type 2 Strain with Natural Deletion in E3 Gene [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2982-2990.
[4]	CAO Xiuyun, LIU Jiwen, TANG Zhihui, ZHENG Ziyi, YAN Liping, SONG Suquan. Isolation, Identification and Pathogenicity Analysis of a Duck Adenovirus Type 3 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2050-2061.
[5]	YUAN Sheng, LI Anqi, Lü Wenke, YANG Lulu, ZHOU Feng, HUANG Liangzong, BAI Aiquan, WEN Feng, HUANG Shujian, GUO Jinyue. Analysis of Variation in Major Virulence-related Genes of a Strain of Pseudorabies Virus and Its Pathogenicity to Rabbits [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2195-2199.
[6]	KU Xugang, YU Xuexiang, SUN Qi, LI Panpan, ZHANG Mengjia, LUO Rui, QIAN Ping, HE Qigai. Isolation and Pathogenicity Analysis of Porcine Circovirus Type 3 [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1568-1578.
[7]	NI Zheng, YE Weicheng, CHEN Liu, YUN Tao, HUA Jionggang, ZHU Yinchu, ZHANG Cun. Genetic Variation and Pathogenicity of a Pseudorabies Virus Variant Strain in Mice [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1766-1770.
[8]	FAN Chenyu, SHAN Yanju, ZHANG Ming, JI Gaige, JU Xiaojun, TU Yunjie, HE Xi, SHU Jingting, LIU Yifan, ZHANG Haihan. Genome-wide Association Study of Body Weight and Meat Quality Traits in Lihua Mahuang Chickens [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(12): 4982-4992.
[9]	YANG Menglin, ZHENG Shiqi, PENG Kai, WANG Wei, HUANG Yanhua, PENG Jie. Isolation and Identification of Pigeon-derived Salmonella Typhimurium and Pathogenic Analysis [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4880-4888.
[10]	HE Chenxiang, GAO Shandian, TIAN Zhancheng, DU Junzheng, WANG Jinming, GUAN Guiquan, YIN Hong. Development of a Differential Diagnostic ELISA Based on G_NS Protein to Distinguish BEFV Infected and Vaccinated Cattle [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4320-4326.
[11]	LI Shun, LI Guizhen, YUAN Yaoxian, LI Kangjian, MA Chunquan, LI Shoujun, HUANG Liangzong, MA Jun. Effect of the Length of PA-X Gene on Replication and Pathogenicity of H3N2 Canine Influenza Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 272-280.
[12]	CHENG Baoyu, LI Zihe, CUI Yanlei, LI Jiahui, YANG Xinwei, YU Yongle, YANG Haiyan, SHAN Hu, ZHANG Chuanmei. Genetic Evolution of Feline Parvovirus and Pathogenicity of an Isolated Strains [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 3121-3131.
[13]	CHANG Zhaoyang, LIU Yumei, LIU Weiqi, WANG Jianing, WEI Lan, ZHANG Ziqiang. Diagnosis of a Rabbit Hemorrhagic Virus Type 2 Infection Epidemic [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1886-1894.
[14]	HAO Jianwei, XUE Chunyi, CAO Yongchang. The Dose-titration Study of Inactivated Vaccine of Porcine Epidemic Diarrhea Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(5): 1536-1543.
[15]	WANG Jianlin, JIANG Zhiyao, LUAN Qingdong, YIN Yanbo. Comparative Pathological Analysis of SPF Chicken Host Infected with Serotype 4, 8a, 8b and 11 Fowl Adenovirus Popular in China [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(5): 1598-1607.