PA-X基因的长度变化对H3N2犬流感病毒复制能力和致病性的影响

doi:10.11843/j.issn.0366-6964.2023.01.025

Abstract

Abstract: H3N2 canine influenza virus (H3N2 CIV) circulate in dogs from multiple area in China, which is a novel branch formed by trans-host infection of avian influenza. Many research showed that the PA-X gene may play roles in the adaptation of influenza A viruses to new hosts, and can impact the replication efficient and virulence of influenza A virus by its length changing. To better understand the function of PA-X gene in H3N2 CIV, eight-plasmid operating systems were used to rescue three recombinant viruses, the parental virus CIV_PA-X_232 strain (232 aa), the CIV_PA-X_Knock strain, and the CIV_PA-X_252 strain (252 aa). To explore the effect of the length change of PA-X gene on H3N2 CIV, we compared the viral polymerase activity, replication efficiency in MDCK cells, and pathogenicity in mice of the three recombinant viruses. In vitro, the polymerase activity of CIV_PA-X_252 and CIV_PA-X_Knock were significantly (P<0.05) higher than that of CIV_PA-X_232, and that of CIV_PA-X_252 was higher than that of CIV_PA-X_Knock; the replication ability of CIV_PA-X_252 and CIV_PA-X_Knock were significant (P<0.05) higher than that of CIV_PA-X_232 in MDCK cells. In vivo, three recombinant viruses had no significant difference in the body weight of mice and were not lethal; TCID₅₀ of three recombinant viruses could be only detected in the lung of infected mice on 1 dpi, and the titer of CIV_PA-X_252 and CIV_PA-X_Knock was slightly higher than CIV_PA-X_232 with no significant difference; three recombinant viruses all caused lung pathological damage in mice, in terms of pathogenicity, CIV_PA-X_252> CIV_PA-X_232> CIV_PA-X_Knock. These results showed that the expression of PA-X protein with different size can affected the H3N2 CIV in transcription, replication, and the pathogenicity of mice. This study preliminarily explored the effects of PA-X gene within different length by H3N2 CIV, providing a reference for subsequent studies on the pathogenesis of H3N2 CIV.

Key words: canine influenza, H3N2, reverse genetic system, PA-X, pathogenicity

CLC Number:

S852.659.5

LI Shun, LI Guizhen, YUAN Yaoxian, LI Kangjian, MA Chunquan, LI Shoujun, HUANG Liangzong, MA Jun. Effect of the Length of PA-X Gene on Replication and Pathogenicity of H3N2 Canine Influenza Virus[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 272-280.

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Effect of the Length of PA-X Gene on Replication and Pathogenicity of H3N2 Canine Influenza Virus

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