我国H9N2亚型禽流感病毒血凝素蛋白145和153位抗原位点变异分析

doi:10.11843/j.issn.0366-6964.2022.04.016

Abstract

Abstract: Antigenic drift on hemagglutinin (HA) protein of H9N2 subtype avian influenza virus (AIV) happens frequently, however, the key antigenic sites for identification of antigenic changes of H9N2 subtype AIV in China remain unclear. Two monoclonal antibodies(mAbs)2E4 and 2D6 with high hemagglutination inhibition (HI) titers against H9N2 subtype AIV were used, and antigenic sites were identified by selection of mAbs escape mutants with H9N2 AIV strain A/Chicken/Shanghai/F/1998. The antigenic profile of representative strains of H9N2 subtype AIV in 2017-2019 was investigated by HI assay using mAbs 2E4 or 2D6. The relationship between antigenic sites and genotype and variation characteristic of antigenic sites for H9N2 AIV in China were also analyzed. Two antigen sites 145 and 153 on the globe of HA protein of H9N2 AIV were identified by mAbs 2E4 or 2D6. H9N2 AIVs isolated in Eastern China in 2017-2019 were divided into two major branches:Group 1 and Group 2, according to the genetic distance of the HA gene. The representative viruses in Group 1 had good reactoin with two mAbs. In contrast, the viruses in Group 2 had poor reaction with two mAbs. Sequence analysis showed that S145D (16/16) and D153G (15/16) mutation happened in the viruses of Group 2. Natural mutation rate analysis showed that the rates of H9N2 subtype AIV strains isolated in China in 1996-2021 with 145D or 153G have risen to 68% or 66%, respectively, which become prevailing strains. The mAb 2E4 may be a tool for antigenic typing of the prevailing H9N2 subtype AIV, and antigenic site 145 may be one of molecular target to identify the difference between Group 1 and Group 2.

Key words: H9N2 subtype AIV, HA, antigenic variation, escape mutant from monoclonal antibody, antigenic site

CLC Number:

S852.659.5

CHEN Zixuan, ZHANG Nan, HU Qun, QUAN Keji, QIN Tao, CHEN Sujuan, PENG Daxin, LIU Xiufan. Mutation Analysis of Antigen Sites 145 and 153 on Hemagglutinin of H9N2 Avian Influenza Virus in China[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(4): 1165-1172.

References 20

[1]	GU M, CHEN H Z, LI Q H, et al. Enzootic genotype S of H9N2 avian influenza viruses donates internal genes to emerging zoonotic influenza viruses in China[J]. Vet Microbiol, 2014, 174(3-4):309-315.
[2]	YU Q, LIU L Q, PU J, et al. Risk perceptions for avian influenza virus infection among poultry workers, China[J]. Emerg Infect Dis, 2013, 19(2):313-316.
[3]	LI C J, YU K Z, TIAN G B, et al. Evolution of H9N2 influenza viruses from domestic poultry in Mainland China[J]. Virology, 2005, 340(1):70-83.
[4]	XU C, YE H, QIU W, et al. Phylogenetic classification of hemagglutinin gene of H9N2 avian influenza viruses isolated in China during 2012-2016 and evaluation of selected candidate vaccine strains[J]. Poult Sci, 2018, 97(9):3023-3030.
[5]	孟芳, 徐怀英, 张伟, 等. 近20年中国部分地区鸡源H9N2亚型禽流感病毒HA基因遗传演化及其变异频率[J]. 微生物学报, 2016, 56(1):35-43.MENG F, XU H Y, ZHANG W, et al. Genetic evolution and substitution frequency of avian influenza virus HA gene in chicken H9N2 subtype in China in the last 20 years[J]. Acta Microbiologica Sinica, 2016, 56(1):35-43. (in Chinese)
[6]	WEI Y D, XU G L, ZHANG G Z, et al. Antigenic evolution of H9N2 chicken influenza viruses isolated in China during 2009-2013 and selection of a candidate vaccine strain with broad cross-reactivity[J]. Vet Microbiol, 2016, 182:1-7.
[7]	HENSLEY S E, DAS S R, BAILEY A L, et al. Hemagglutinin receptor binding avidity drives influenza A virus antigenic drift[J]. Science, 2009, 326(5953):734-736.
[8]	PEACOCK T, REDDY K, JAMES J, et al. Antigenic mapping of an H9N2 avian influenza virus reveals two discrete antigenic sites and a novel mechanism of immune escape[J]. Sci Rep, 2016, 6:18745.
[9]	PEACOCK T P, HARVEY W T, SADEYEN J R, et al. The molecular basis of antigenic variation among A(H9N2) avian influenza viruses[J]. Emerg Microbes Infect, 2018, 7(1):176.
[10]	鞠勇. 2013-2015年华东地区H9N2禽流感病毒的流行病学调查及单克隆抗体的研制[D]. 扬州:扬州大学, 2016.JU Y. Epidemiological Investigation of H9N2 avian influenza viruses in the Eastern China from 2013 to 2015 and development of monoclonal antibodies against H9N2 avian influenza virus[D]. Yangzhou:Yangzhou University, 2016. (in Chinese)
[11]	孙兰. H9N2亚型禽流感病毒HA和NP蛋白单克隆抗体的制备及在抗原变异分析中的初步应用[D]. 扬州:扬州大学, 2020.SUN L. Preparation of monoclonal antibodies against HA and NP protein of H9N2 subtype avian influenza virus and its preliminary application in antigen variation analysis[D]. Yangzhou:Yangzhou University, 2020. (in Chinese)
[12]	STEEL J, LOWEN A C, WANG T T, et al. Influenza virus vaccine based on the conserved hemagglutinin stalk domain[J]. mBio, 2010, 1(1):e00018-10.
[13]	ZHU Y B, YANG D, REN Q, et al. Identification and characterization of a novel antigenic epitope in the hemagglutinin of the escape mutants of H9N2 avian influenza viruses[J]. Vet Microbiol, 2015, 178(1-2):144-149.
[14]	GERHARD W, WEBSTER R G. Antigenic drift in influenza A viruses. I. Selection and characterization of antigenic variants of A/PR/8/34 [H0N1] influenza virus with monoclonal antibodies[J]. J Exp Med, 1978, 148(2):383-392.
[15]	LETUNIC I, BORK P. Interactive tree of life (iTOL) v5:an online tool for phylogenetic tree display and annotation[J]. Nucleic Acids Res, 2021, 49(W1):W293-W296.
[16]	KAVERIN N V, RUDNEVA I A, ILYUSHINA N A, et al. Structural differences among hemagglutinins of influenza A virus subtypes are reflected in their antigenic architecture:analysis of H9 escape mutants[J]. J Virol, 2004, 78(1):240-249.
[17]	SEALY J E, PEACOCK T P, SADEYEN J R, et al. Adsorptive mutation and N-linked glycosylation modulate influenza virus antigenicity and fitness[J]. Emerg Microbes Infect, 2020, 9(1):2622-2631.
[18]	XIONG X L, CORTI D, LIU J F, et al. Structures of complexes formed by H5 influenza hemagglutinin with a potent broadly neutralizing human monoclonal antibody[J]. Proc Natl Acad Sci U S A, 2015, 112(30):9430-9435.
[19]	CATON A J, BROWNLEE G G, YEWDELL J W, et al. The antigenic structure of the influenza virus A/PR/8/34 hemagglutinin (H1 subtype)[J]. Cell, 1982, 31(2):417-427.
[20]	申松玮, 王泽源, 范威峰, 等. 2017—2018年华东地区H9亚型禽流感病毒分离毒株的抗原差异分析[J]. 畜牧兽医学报, 2019, 50(6):1230-1238.SHEN S W, WANG Z Y, FAN W F, et al. Antigen difference analysis of H9 subtype avian influenza viruses isolated in East China during 2017-2018[J]. Chinese Journal of Animal and Veterinary Sciences, 2019, 50(6):1230-1238. (in Chinese)

Mutation Analysis of Antigen Sites 145 and 153 on Hemagglutinin of H9N2 Avian Influenza Virus in China

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 20

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	CHONG Qian, ZHAO Xuehui, CAO Qing, XUE Huiwen, GOU Huitian. Research Progress of Applied Research on Listeria monocytogenes Phage [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 1819-1826.
[2]	NIU Jiajia, XU Dan, LIU Yang, ZHAO Xiaoling. Research Progress on Genetic Regulation Mechanism of Barring Feather Trait in Chicken [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 1883-1892.
[3]	YU Zuhua, GAO Mengru, QI Zhiying, ZHANG Jingyu, HE Lei, CHEN Jian, DING Ke. Research Progress on the Function of RNA Binding Protein ELAVL1 and Its Regulation of Viral Replication [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 1914-1925.
[4]	ZHANG Jixian, FAN Dingkun, FU Yuze, JIAO Shuai, MA Tao, BI Yanliang, ZHANG Naifeng. Research Progress on Mechanism and Application of Postbiotics in Regulating Animal Intestinal Health [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 1926-1935.
[5]	LÜ Yadi, YANG Jie, XIE Wenting, XU Ting, CHEN Ruiai. Construction and Evaluation of the Immune Effect of Recombinant Genotype Ⅶ NDV Strain Co-expressing Membrane-bound and Water-soluble HA Protein of Avian Influenza Virus H9N2 [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2123-2134.
[6]	GAO Jie, LI Xiaocheng, MU Yang, ZHANG Hui, WEI Rong, LI Jie. Biological Characteristics and Immune Effect Evaluation of Outer Membrane Vesicles of Capsular Type B Pasteurella multocida [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2168-2175.
[7]	FEI Guoqing, NING Zhiyuan, ZHAO Zefang, LIU Yanqiu, LIU Tengfei, LI Xian, CONG Rihua, CHEN Hong, CHEN Shulin. Isolation, Identification of Luteal Cells from Cows during Pregnancy and Investigation of Their Culture Characteristics [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2214-2225.
[8]	LIU Jiahui, WU Kaikai, WANG Lei, ZHANG Kang, HAN Songwei, CHEN Fubin, XU Guowei, GUO Zhiting, GU Xueyan, ZHANG Jingyan, LI Jianxi. Protective Effects of Astragalus Polysaccharides, Saponins and Probiotic Compounds on Intestinal Tract of Broilers Infected with E.coli [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(5): 2241-2252.
[9]	HUANG Jie, RUAN Zihao, CAI Rui. Advances of the Application of Antimicrobial Peptides in the Preservation of Porcine Semen at Room Temperature [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1401-1411.
[10]	HE Xiaolan, ZHAO Yankun, MENG Lu, LIU Huimin, GAO Jiaojiao, ZHENG Nan. Research Progress in Heteroresistance of Staphylococcus aureus [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1432-1445.
[11]	LI Feifei, ZHANG Chenmiao, TONG Jinjin, JIANG Linshu. Research Progress on the Mechanism of Mitochondrial Autophagy Regulating the Activity of NLRP3 Inflammatory Corpuscles to Improve Animal Health [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1446-1455.
[12]	LUO Tongwang, WU Ya, WANG Shujie, SONG Houhui, SHAO Chunyan. Research Progress on the Mechanism of Cadmium Induced Liver Damage and Selenium Antagonizing Cadmium Hepatotoxicity [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1456-1466.
[13]	ZHANG Shaohua, WANG Shuai, ZOU Yang, LIU Zhongli, CAI Xuepeng. Advances in Detection Approaches for Ovine Haemonchosis [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1499-1510.
[14]	ZHANG Yanmin, ZHAO Dongxu, WANG Wenlong. Mechanism of Resistance to Ivermectin in the Haemonchus contortus [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1511-1520.
[15]	CAO Yuzhu, XING Yuxin, MA Chenglin, GUAN Hongbo, JIA Qihui, KANG Xiangtao, TIAN Yadong, LI Zhuanjian, LIU Xiaojun, LI Hong. Biological Characterization of Chicken FGF6 Gene and Association of Its Polymorphisms with Economic Traits [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(4): 1536-1550.