小熊猫源犬瘟热病毒株H、F基因的克隆及序列分析

doi:10.11843/j.issn.0366-6964.2021.06.021

Abstract

Abstract: To understand the genetic variation of a strain (GD-1) of canine distemper virus (CDV) from captive red panda, the H and F genes of the CDV were cloned by RT-PCR, then were sequenced and analyzed. The results showed that the H gene sequence of this isolate had the highest nucleotide similarity (96%) with the CDV strain (accession number:GU266280) reported from Denmark in GenBank, and the F gene sequence had the highest nucleotide similarity with the CDV strain (accession number:KY057355) reported from Brazil. The sequences of CDV representative strains were downloaded for genetic evolution, amino acid sequence alignment and molecular characterization analysis. The results showed that there were 8 potential N-glycation sites in H protein, which were located at sites 19, 149, 309, 391, 422, 456, 587 and 603, respectively. The amino acid sequence of the SLAM receptor binding site of H protein was the same as that of Eurasian wild type virulent strain. Compared with the vaccine strains, the amino acid sequences at positions 530, 549 were different. Compared with other CDV reference strains, there were significant variations in H protein at 24 and 41 sites. The amino acid homology between H protein and standard virulent strain A75/17 was 95.2%. The amino acid sequence similarity with five vaccine strains such as Onderstepoort and Convac was 88.2%-89.3%. There were six N-glycosylation sites in F protein, which were located at 62, 108, 141, 173, 179 and 517 sites, respectively. The amino acid similarity between F protein and other vaccine strains ranged from 89.1% to 89.7%. Compared with other reference strains, there were eleven amino acid mutations in 115, 130 and so on. The genetic evolution tree based on H and F genes was constructed. The results showed that the strain was located in a small evolutionary branch of Asia-4 type, which was different from the current epidemic strains mainly located in Asia-1 type in China. In this study, the Asia-4 genotype CDV wild strain from red panda was reported for the first time, and the H and F genes of the strain were sequenced, which is of great significance for the study of genetic variation, epidemiological investigation, disease prevention and control and vaccine development of CDV epidemic strains in China.

Key words: red panda, canine distermper, H gene, F gene, sequence analysis

CLC Number:

S852.659.5

JIANG Mei, CHEN Wu, ZHAI Junqiong, BU Wandi, XIE Yilun, LIU Canbin, SHAN Fen, LUO Manlin. Cloning and Sequence Analysis of H and F Genes of Canine Distemper Virus Strain from Red Panda[J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(6): 1670-1676.

References 31

[1]	CHENG Y N, WANG J K, ZHANG M, et al. Isolation and sequence analysis of a canine distemper virus from a raccoon dog in Jilin Province, China[J]. Virus Genes, 2015, 51(2):298-301.
[2]	BEINEKE A, BAUMGÄRTNER W, WOHLSEIN P. Cross-species transmission of canine distemper virus-an update[J]. One Health, 2015, 1:49-59.
[3]	LI W K, CAI C, XUE M Z, et al. Phylogenetic analysis of canine distemper viruses isolated from vaccinated dogs in Wuhan[J]. J Vet Med Sci, 2018, 80(11):1688-1690.
[4]	MAGANGA G D, LABOUBA I, NGOUBANGOYE B, et al. Molecular characterization of complete genome of a canine distemper virus associated with fatal infection in dogs in Gabon, Central Africa[J]. Virus Res, 2018, 247:21-25.
[5]	PARDO I D R, JOHNSON G C, KLEIBOEKER S B. Phylogenetic characterization of canine distemper viruses detected in naturally infected dogs in North America[J]. J Clin Microbiol, 2005, 43(10):5009-5017.
[6]	BAE C W, LEE J B, PARK S Y, et al. Deduced sequences of the membrane fusion and attachment proteins of canine distemper viruses isolated from dogs and wild animals in Korea[J]. Virus Genes, 2013, 47(1):56-65.
[7]	FREITAS L A, LEME R A, SAPORITI V, et al. Molecular analysis of the full-length F gene of Brazilian strains of canine distemper virus shows lineage co-circulation and variability between field and vaccine strains[J]. Virus Res, 2019, 264:8-15.
[8]	ROMANUTTI C, KELLER L, LA TORRE J, et al. Virus isolation and full-length genome sequencing of a representative canine distemper virus wild type strain of the South America 2 clade[J]. J Virol Methods, 2020, 279:113857.
[9]	IWATSUKI K, TOKIYOSHI S, HIRAYAMA N, et al. Antigenic differences in the H proteins of canine distemper viruses[J]. Vet Microbiol, 2000, 71(3-4):281-286.
[10]	QIN Q, WEI F W, LI M, et al. Serosurvey of infectious disease agents of carnivores in captive red pandas (Ailurus fulgens) in China[J]. J Zoo Wildl Med, 2007, 38(1):42-50.
[11]	PANTHI S, WANG T J, SUN Y W, et al. An assessment of human impacts on endangered red pandas (Ailurus fulgens) living in the Himalaya[J]. Ecol Evol, 2019, 9(23):13413-13425.
[12]	MICKWITZ V. Distemper in procyonidae and pandas[J]. Kleintier-Prax, 1968, 13(3):80-90.
[13]	左奕, 郑英帅. 小熊猫犬瘟热的流行与防控[J]. 黑龙江畜牧兽医, 2018(24):216-218.ZUO Y, ZHENG Y S. The epidemic and control of canine distemper in red panda[J]. Heilongjiang Animal Science and Veterinary Medicine, 2018(24):216-218. (in Chinese)
[14]	刘彩红, 曹玉姣, 丁航天, 等. 水貂犬瘟热病毒分离鉴定及其H基因序列分析[J]. 动物医学进展, 2020, 41(1):27-33.LIU C H, CAO Y J, DING H T, et al. Isolation and identification of canine distemper virus in minks and sequence analysis of H gene[J]. Progress in Veterinary Medicine, 2020, 41(1):27-33. (in Chinese)
[15]	林希, 刘若寒, 郝香琪, 等. 广东地区犬瘟热病毒血凝素基因的克隆与序列分析[J]. 华南农业大学学报, 2019, 40(6):22-28.LIN X, LIU R H, HAO X Q, et al. Cloning and sequence analysis of haemagglutinin gene of canine distemper virus in Guangdong area[J]. Journal of South China Agricultural University, 2019, 40(6):22-28. (in Chinese)
[16]	SUZUKI J, NISHIO Y, KAMEO Y, et al. Canine distemper virus infection among wildlife before and after the epidemic[J]. J Vet Med Sci, 2015, 77(11):1457-1463.
[17]	JIN Y P, ZHANG X K, MA Y S, et al. Canine distemper viral infection threatens the giant panda population in China[J]. Oncotarget, 2017, 8(69):113910-113919.
[18]	ANIS E, NEEDLE D B, STEVENS B, et al. Genetic characteristics of canine distemper viruses circulating in wildlife in the United States[J]. J Zoo Wildl Med, 2020, 50(4):790-797.
[19]	RILEY M C, WIIKES R P. Sequencing of emerging canine distemper virus strain reveals new distinct genetic lineage in the United States associated with disease in wildlife and domestic canine populations[J]. Virol J, 2015, 12(1):219.
[20]	陈美荣, 林伟东, 宋天琪, 等. 犬瘟热病毒BJ16B2株的分离鉴定及H基因遗传进化分析[J]. 畜牧与兽医, 2018, 50(8):82-89.CHEN M R, LIN W D, SONG T Q, et al. Isolation and identification of the canine distemper virus BJ16B2 strain and the genetic variability analysis of its H gene[J]. Animal Husbandry & Veterinary Medicine, 2018, 50(8):82-89. (in Chinese)
[21]	卜研, 薛向红, 闫喜军, 等. 狐源犬瘟热病毒HBF-1株全基因组序列测定及其分析[J]. 中国兽医学报, 2020, 40(3):510-516, 540.BU Y, XUE X H, YAN X J, et al. Full-length genome sequencing and analysis of Vero-dSlam cell-adapted canine distemper virus HBF-1 strain[J]. Chinese Journal of Veterinary Science, 2020, 40(3):510-516, 540. (in Chinese)
[22]	CHEN M, XIN T, HOU S, et al. Genotyping and pathogenic characterization of canine distemper virus based on mutations in the hemagglutinin gene in Chinese domestic dogs[J]. Pol J Vet Sci, 2018, 21(3):623-629.
[23]	TAO R S, BA H X, CHEN J, et al. Phylodynamic analysis of two amino acid substitutions in the hemagglutinin protein of canine distemper virus strains detected in fur-bearing animals in China[J]. Virus Genes, 2020, 56(1):58-66.
[24]	MONTALI R J, BARTZ C R, TEARE J A, et al. Clinical trials with canine distemper vaccines in exotic carnivores[J]. J Am Vet Med Assoc, 1983, 183(11):1163-1167.
[25]	CALDERON M G, REMORINI P, PERIOLO O, et al. Detection by RT-PCR and genetic characterization of canine distemper virus from vaccinated and non-vaccinated dogs in Argentina[J]. Vet Microbiol, 2007, 125(3-4):341-349.
[26]	PIEWBANG C, RADTANAKATIKANON A, PUENPA J, et al. Genetic and evolutionary analysis of a new Asia-4 lineage and naturally recombinant canine distemper virus strains from Thailand[J]. Sci Rep, 2019, 9(1):3198.
[27]	DUQUE-VALENCIA J, FORERO-MUÑOZ N R, DÍAZ F J, et al. Phylogenetic evidence of the intercontinental circulation of a Canine distemper virus lineage in the Americas[J]. Sci Rep, 2019, 9(1):15747.
[28]	SWATI, DEKA D, UPPAL S K, et al. Isolation and phylogenetic characterization of Canine distemper virus from India[J]. VirusDisease, 2015, 26(3):133-140.
[29]	YUAN C W, LIU W X, WANG Y B, et al. Homologous recombination is a force in the evolution of canine distemper virus[J]. PLoS One, 2017, 12(4):e0175416.
[30]	熊焰, 徐志文, 王印, 等. 小熊猫犬瘟热病及病原研究[J]. 四川动物, 2000, 19(5):13-15.XIONG Y, XU Z W, WANG Y, et al. The study on the distemper and pathogen of canine distemper in red panda[J]. Sichuan Journal of Zoology, 2000, 19(5):13-15. (in Chinese)
[31]	ZHANG H, SHAN F, ZHOU X, et al. Outbreak and genotyping of canine distemper virus in captive Siberian tigers and red panda[J]. Sci Rep, 2017, 7(1):8132.

Cloning and Sequence Analysis of H and F Genes of Canine Distemper Virus Strain from Red Panda

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 31

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	CAO Liyan, KONG Xiangyu, LI Xiangtong, SUO Xuepeng, DUAN Yueyue, YUAN Cong, SHI Lei, ZHANG Yu, MA Guoxiang, ZHENG Haixue, WANG Qi. Preparation and Sequence Analysis of Monoclonal Antibody against the Nucleocapsid Protein of Porcine Acute Diarrhea Syndrome Coronavirus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2487-2497.
[2]	YUAN Sheng, LI Anqi, Lü Wenke, YANG Lulu, ZHOU Feng, HUANG Liangzong, BAI Aiquan, WEN Feng, HUANG Shujian, GUO Jinyue. Analysis of Variation in Major Virulence-related Genes of a Strain of Pseudorabies Virus and Its Pathogenicity to Rabbits [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2195-2199.
[3]	LI Chang, ZHOU Ping, WEN Ping, YANG Keli, LIU Wei, GUO Rui, LIU Zewen, YUAN Fangyan, GAO Ting, SONG Haofei, TIAN Yongxiang, ZHOU Danna. Investigation of the Genome-wide Sequence and Immunogenicity of Capsid Protein in Mice for a Porcine Circovirus 2d Genotype Strain [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4289-4299.
[4]	LIANG Gaoxing, RONG Shiqi, WANG Junwei, LI Yuan, YANG Xin, ZHAO Guanghui, SONG Junke. Multi-locus Gene Sequence Analysis of Female Haemonchus contortus with Morphological Differences in Vulval Flap from Goat [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 3140-3148.
[5]	GAO Xin, LUO Yuanyuan, YANG Huiming, ZHANG Guozhong, ZHAO Ye. Genomic Characteristics and Pathogenicity of an Avian Infectious Laryngotracheitis Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(4): 1298-1302.
[6]	HE Jian, XING Xiaoyong, WU Xiaochun, WEN Fengqin, ZHANG Yangyang, ZHANG Shengying, LIU Jia, BAO Shijun. Isolation and Identification of a Strain of Pseudomonas aeruginosa from Forest Musk and Analysis of the Whole Genome Sequence [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(9): 2569-2578.
[7]	HOU Jing, SHEN Caocao, ZHANG Dajun, YANG Bo, SHI Xijuan, ZHANG Ting, CUI Huimei, YUAN Xingguo, ZHAO Dengshuai, CHEN Xuehui, ZHANG Keshan, ZHENG Haixue, LIU Xiangtao. Gene Sequence Analysis, Protein Structure Prediction and Subcellular Localization of African Swine Fever Virus Helicase D1133L [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(7): 1953-1962.
[8]	ZHAO Caiqin, WANG Yanbi, ZHU Jie, TANG Hong, DONG Yuntao, DUAN Zhiqiang. Sequence Analysis and Interaction Verification of Chicken TRAF6 and TIFA Proteins [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(6): 1511-1522.
[9]	LI Ning, GUO Huifang, WANG Baiyu, QIAO Qilong, HUANG Qing, LI Yongtao, WANG Zeng, ZHAO Jun. Genome Sequencing and Phylogenetic Analysis of Senecavirus A, CH-HNCY-2019 [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(2): 553-559.
[10]	ZHANG Chunmei, JIA Jianlei, XIE Wen, REN Hao, ZHANG Huaixia, ZHANG Yingying, CHEN Qian. Construction of Tibetan Sheep HSP27 Gene Overexpression and Silencing Vectors and Preliminary Analysis of Its Function on Follicular Development [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(12): 3413-3425.
[11]	CHEN Qingyi, CONG Guanglei, XIAO Yunqi, SHI Shourong. Molecular Cloning, Sequence Analysis and Expression Characteristics of Angiotensin Converting Enzyme 2 (ACE2) Gene in Xueshan Chicken [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(11): 3053-3063.
[12]	YANG Zhenxing, XIE Jiarui, LI Zhanhong, LI Zhuoran, LIAO Defang, NIU Baosheng, YAO Pingfen, LI Le, YANG Heng. Isolation and Identification of a Novel Type Tibet Orbivirus Strain from Culicoides Collected in Shizong County of Yunnan Province [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(1): 166-176.
[13]	LI Shuai, JI Xiaoxia, YANG Bo, XU Meng, ZHANG Yuanshu. Gene Cloning and Sequence Analysis of Angiotensin Converting Enzyme 2 (ACE2) in China Sheldrake Duck [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(7): 1597-1606.
[14]	SHEN Chaochao, LI Guoli, ZHANG Dajun, XU Guowei, HOU Jing, LI Dan, DANG Wen, ZHANG Keshan, ZHENG Haixue, LIU Xiangtao. Gene Sequence Analysis, Protein Structure Prediction and Subcellular Localization of MGF 360-9L from African Swine Fever Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(6): 1371-1381.
[15]	ZHAO Shuo, WANG Ruomu, DANG Jiajia, XU Lishi, QIN Shuying, XUE Hui, WEI Zuzhang, CHEN Ying, OUYANG Kang, HUANG Weijian. Genetic Variation Analysis of gB, gE and TK Genes of Pseudorabies Virus in Guangxi from 2013 to 2018 [J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(4): 810-819.