IFITM3对小反刍兽疫病毒在山羊子宫内膜上皮细胞中增殖的调控效应

doi:10.11843/j.issn.0366-6964.2023.05.040

Abstract

Abstract: Interferon-induced transmembrane protein 3 (IFITM3) plays an important role in the regulation of viral infection. However, the role of IFITM3 in peste des petits ruminants virus (PPRV) infection remains unclear. This study aimed to investigate the effects of IFITM3 on virus infection in goat endometrial epithelial cells (EECs) infected with PPRV. We performed Western blot assay to detect the kinetics of IFITM3 expression in PPRV-infected EECs. Then, the effect of IFITM3 on PPRV replication was further explored by gene overexpression and knockdown techniques. Our data showed that, compared with mock-infected cells, virus replication increased in PPRV-infected cells in post infection time-dependent manner, which was accompanied with an increased IFITM3 expression followed by decreased expression. The expression of IFITM3 levels increased starting at 2 hours post infection (hpi) (P<0.001), and reached peak at 3-4 hpi (P<0.0001). At 24 hpi, there was no significant difference in IFITM3 expression between PPRV-and mock-infected cells. Moreover, IFITM3 silencing with siRNA significantly inhibit PPRV N expression levels compared to negative control cells at 0-6 hpi, particularly, at 6 hpi. In contrast, over expression of IFITM3 by pcDNA3.1-IFITM3 transfection enhanced PPRV replication levels compared to untransfected cells at 6 hpi (P<0.001). There was no significant difference of virus replication levels in pcDNA3.1-IFITM3 transfected-cells compared to negative control at 12-24 hpi. Together, these results demonstrate that PPRV-induced IFITM3 expression facilitate virus replication during early infection.

Key words: interferon-inducible transmembrane protein 3, peste des petits ruminants virus, Endometriumepithelialcells, virus replication

CLC Number:

S852.659.5

FANG Yuan, HOU Qiaodi, XIANG Chaohui, ZHAO Hongyi, QI Xuefeng. Regulatory Effects of IFITM3 on Proliferation of Peste des Petits Ruminants Virus (PPRV) in Goat Endometrial Epithelial Cells[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2200-2207.

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Regulatory Effects of IFITM3 on Proliferation of Peste des Petits Ruminants Virus (PPRV) in Goat Endometrial Epithelial Cells

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