组蛋白去乙酰化酶抑制剂对小反刍兽疫病毒复制的影响

doi:10.11843/j.issn.0366-6964.2022.07.027

Abstract

Abstract: The present study aimed to explore the effect of histone deacetylase(HDAC) inhibitors on peste des petits ruminants virus (PPRV) replication, and to determine the role of HDACs involved in the PPRV replication.The mRNA expression of HDACs (HDAC1-11) was examined by RT-qPCR assay in PPRV-infected Vero cells. To screen the inhibitors that affect PPRV replication, the expression level of N protein was evaluated in infected Vero cells by Western blot following treatment with the various inhibitors for different types of HDACs for 48 h. The selected inhibitors were used to treat PPRV-infected Vero cells, and the effects of inhibitors on PPRV N RNA, N protein and virus titer were further analyzed by RT-qPCR, Western blot and TCID₅₀, respectively. The results showed that PPRV infection in Vero cells resulted in a significant increase of the mRNA expression level of HDAC2 (P<0.05). After treatment with SAHA, TMP269 and MGCD0103, the expression level of PPRV N protein was significantly reduced and negatively correlated with the doses of these inhibitors. SAHA, TMP269 and MGCD0103 also significantly reduced the expression level of PPRV N RNA (P<0.05, P<0.05, P<0.01) and virus titer (P<0.05, P<0.05, P<0.01), respectively. These results indicated that class IIa HDACs inhibitors and class I HDACs inhibitors significantly inhibited PPRV replication in Vero cells, suggesting that class I HDACs (HDAC1-3) and class IIa HDACs (HDAC4-5, HDAC7, HDAC9) may be involved in the regulation of PPRV infection.

Key words: histone deacetylase inhibitor, histone deacetylase, peste des petits ruminants virus, replication

CLC Number:

S852.659.5

PAN Chunrong, DENG Ruixue, HU Linjie, ZHU Xueliang, SUN Yuefeng, WANG Guirong, MENG Xuelian. Effect of Histone Deacetylase Inhibitors on Peste Des Petits Ruminants Virus Replication[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(7): 2307-2316.

References 31

[1]	GIBBS E P, TAYLOR W P, LAWMAN M J P, et al. Classification of peste des petits ruminants virus as the fourth member of the genus Morbillivirus[J]. Intervirology, 1979, 11(5):268-274.
[2]	MANTIP S E, SHAMAKI D, FAROUGOU S. Peste des petits ruminants in Africa:Meta-analysis of the virus isolation in molecular epidemiology studies[J]. Onderstepoort J Vet Res, 2019, 86(1):e1-e15.
[3]	LIU F, LI J, LI L, et al. Peste des petits ruminants in China since its first outbreak in 2007:A 10-year review[J]. Transbound Emerg Dis, 2018, 65(3):638-648.
[4]	LI L X, CAO X A, WU J Y, et al. Epidemic and evolutionary characteristics of peste des petits ruminants virus infecting Procapra przewalskii in Western China[J]. Infect Genet Evol, 2019, 75:104004.
[5]	DE RUIJTER A J M, VAN GENNIP A H, CARON H N, et al. Histone deacetylases (HDACs):characterization of the classical HDAC family[J]. Biochem J, 2003, 370(Pt 3):737-749.
[6]	YANG X J, GRÉGOIRE S. Class II histone deacetylases:from sequence to function, regulation, and clinical implication[J]. Mol Cell Biol, 2005, 25(8):2873-2884.
[7]	ZHOU Y, WANG Q, YANG Q, et al. Histone deacetylase 3 inhibitor suppresses hepatitis c virus replication by regulating Apo-A1 and LEAP-1 expression[J]. Virol Sin, 2018, 33(5):418-428.
[8]	LU C Y, CHANG Y C, HUA C H, et al. Tubacin, an HDAC6 Selective inhibitor, reduces the replication of the japanese encephalitis virus via the decrease of viral RNA synthesis[J]. Int J Mol Sci, 2017, 18(5):954.
[9]	SEWACK G F, ELLIS T W, HANSEN U. Binding of TATA binding protein to a naturally positioned nucleosome is facilitated by histone acetylation[J]. Mol Cell Biol, 2001, 21(4):1404-1415.
[10]	KOUZARIDES T. Histone acetylases and deacetylases in cell proliferation[J]. Curr Opin Genet Dev, 1999, 9(1):40-48.
[11]	FENG Q Q, SU Z L, SONG S Y, et al. Histone deacetylase inhibitors suppress RSV infection and alleviate virus-induced airway inflammation[J]. Int J Mol Med, 2016, 38(3):812-822.
[12]	TANG X L, GAO J S, GUAN Y J, et al. Acetylation-dependent signal transduction for type i interferon receptor[J]. Cell, 2007, 131(1):93-105.
[13]	RANDALL R E, GOODBOURN S. Interferons and viruses:an interplay between induction, signalling, antiviral responses and virus countermeasures[J]. J General Virol, 2008, 89(1):1-47.
[14]	YANG Y, YAN Y, CHEN Z, et al. Histone deacetylase inhibitors romidepsin and vorinostat promote Hepatitis B virus replication by inducing cell cycle arrest[J]. J Clin Transl Hepatol, 2021, 9(2):160-168.
[15]	GROZINGER C M, HASSIG C A, SCHREIBER S L. Three proteins define a class of human histone deacetylases related to yeast Hda1p[J]. Proc Natl Acad Sci U S A, 1999, 96(9):4868-4873.
[16]	YANG Q, TANG J L, PEI R J, et al. Host HDAC4 regulates the antiviral response by inhibiting the phosphorylation of IRF3[J]. J Mol Cell Biol, 2019, 11(2):158-169.
[17]	LU Y X, STUART J H, TALBOT-COOPER C, et al. Histone deacetylase 4 promotes type I interferon signaling, restricts DNA viruses, and is degraded via vaccinia virus protein C6[J]. Proc Natl A Sci U S A, 2019, 116(24):11997-12006.
[18]	SODAY L, LU Y X, ALBARNAZ J D, et al. Quantitative temporal proteomic analysis of vaccinia virus infection reveals regulation of histone deacetylases by an interferon antagonist[J]. Cell Rep, 2019, 27(6):1920-1933.
[19]	RAHEEL R, ASHRAF M, EJAZ S, et al. Assessment of the cytotoxic and anti-viral potential of aqueous extracts from different parts of Acacia nilotica (Linn) Delile against Peste des petits ruminants virus[J]. Environ Toxicol Pharmacol, 2013, 35(1):72-81.
[20]	YADAV P, CHOUDHURY S, BARUA S, et al. Polyalthia longifolia leaves methanolic extract targets entry and budding of viruses-an in vitro experimental study against paramyxoviruses[J]. J Ethnopharmacol, 2020, 248:112279.
[21]	KHANDELWAL N, KAUR G, CHAUBEY K K, et al. Silver nanoparticles impair Peste des petits ruminants virus replication[J]. Virus Res, 2014, 190:1-7.
[22]	SUJATHA K, SHANTHI G, SELVAM N P, et al. Synthesis and antiviral activity of 4, 4'-(arylmethylene) bis (1H-pyrazol-5-ols) against peste des petits ruminant virus (PPRV)[J]. Bioorg Med Chem Lett, 2009, 19(15):4501-4503.
[23]	EL-SABBAGH O I, EL-SADEK M E, EL-KALYOUBI S, et al. Synthesis, DNA binding and antiviral activity of new uracil, xanthine, and pteridine derivatives[J]. Arch Pharm (Weinheim), 2007, 340(1):26-31.
[24]	KHANDELWAL N, CHANDER Y, RAWAT K D, et al. Emetine inhibits replication of RNA and DNA viruses without generating drug-resistant virus variants[J]. Antiviral Res, 2017, 144:196-204.
[25]	SAADH M. Epigallocatechin gallate (EGCG) combined with zinc sulfate inhibits Peste des petits ruminants virus entry and replication[J]. Saudi J Biol Sci, 2021, 28(11):6674-6678.
[26]	KUMAR N, KHANDELWAL N, KUMAR R, et al. Inhibitor of sarco/endoplasmic reticulum calcium-ATPase impairs multiple steps of paramyxovirus replication[J]. Front Microbiol, 2019, 10:209.
[27]	ZHANG W F, DENG H L, LIU Y F, et al. Antiviral effectivity of favipiravir against peste des petits ruminants virus is mediated by the JAK/STAT and PI3K/AKT pathways[J]. Front Vet Sci, 2021, 8:722840.
[28]	JIN L, LI Y C, PU F Y, et al. Inhibiting pyrimidine biosynthesis impairs Peste des Petits Ruminants Virus replication through depletion of nucleoside pools and activation of cellular immunity[J]. Vet Microbiol, 2021, 260:109186.
[29]	CHANG Q Y, GUO F C, LI X R, et al. The IMPDH inhibitors, ribavirin and mycophenolic acid, inhibit peste des petits ruminants virus infection[J]. Vet Res Commun, 2018, 42(4):309-313.
[30]	KOBAYASHI Y, GÉLINAS C, DOUGHERTY J P. Histone deacetylase inhibitors containing a benzamide functional group and a pyridyl cap are preferentially effective human immunodeficiency virus-1 latency-reversing agents in primary resting CD4+ T cells[J]. J General Virol, 2017, 98(4):799-809.
[31]	ARCHIN N M, LIBERTY A L, KASHUBA A D, et al. Administration of vorinostat disrupts HIV-1 latency in patients on antiretroviral therapy[J]. Nature, 2012, 487(7408):482-485.

Effect of Histone Deacetylase Inhibitors on Peste Des Petits Ruminants Virus Replication

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References 31

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	SONG Wenyan, ZHANG Hanwen, WU Aodi, ZHANG Liyan, LIU Zhao, YE Tongtong, CHEN Chuangfu, SHENG Jinliang. Screening of Nanobodies against Porcine Reproductive and Respiratory Syndrome Virus GP5 Protein and Exploration of Their Inhibitory Effect on Virus Replication [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 258-270.
[2]	WANG Yu, GAO Yueyi, GAO Jinyuan, LIU Weijie, XU Huilin, XUE Qinghong. Establishment of Reverse Genetics System of Peste des petits Ruminants Virus Clone9 Strain [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2956-2963.
[3]	FANG Yuan, HOU Qiaodi, XIANG Chaohui, ZHAO Hongyi, QI Xuefeng. Regulatory Effects of IFITM3 on Proliferation of Peste des Petits Ruminants Virus (PPRV) in Goat Endometrial Epithelial Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2200-2207.
[4]	WANG Lan, HE Mingyu, ZHANG Min, DING Juntao. MicroRNAs Regulate Antiviral Immunity and Viral Replication [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 463-472.
[5]	CHEN Junzhen, QUAN Ran, FU Qiang, GE Lijuan, YUAN Yuanyuan, ZHANG Chengyuan, LI Jianlin, SHI Huijun. Study on the Effect of Heat Shock Protein HSP90B1 on the Replication of Bovine Viral Diarrhea Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(2): 683-693.
[6]	JIA Yiquan, YU Mengting, LIU Weirong, FANG Shouguo, ZHANG Songbai. Baicalin Inhibits Infectious Bronchitis Virus Replication via Promoting the Expression of Type Ⅰ Interferon [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4839-4850.
[7]	YANG Fuchun, LIU Rui, LI Xiaohan, GAO Li, LIU Changjun, QI Xiaole, CUI Hongyu, WANG Xiaomei, GAO Yulong, LI Kai. Construction of UL41 Gene Deletion Strain of Duck Enteritis Virus and Analysis of Its Replication Ability in vitro [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(8): 2689-2696.
[8]	YUAN Xingguo, SHEN Chaochao, ZHAO Dengshuai, YANG Bo, CUI Huimei, HAO Yu, YANG Jinke, CHEN Xuehui, ZHANG Ting, ZHANG Keshan, LIU Xia, ZHENG Haixue, LIU Xiangtao. β-estradiol and Progesterone Promote Replication of African Swine Fever Virus in vitro [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(7): 2252-2259.
[9]	SHI Huijun, DONG Wenli, GUO Yanting, YUAN Yuanyuan, CHEN Junzhen, YANG Li, RAN Duoliang, FU Qiang. Correlation Analysis of Cell Signal Peptidase Complex Subunit 1 and Bovine Viral Diarrhea Virus Replication [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(6): 1870-1876.
[10]	ZHAO Yujia, CHEN Rui, SONG Daili, ZHANG Luwen, XIAO Dai, LI Shiqian, WEN Yiping, WU Rui, ZHAO Qin, DU Senyan, YAN Qigui, WEN Xintian, CAO Sanjie, HUANG Xiaobo. Effect of Human Aminopeptidase N(hAPN) on Porcine Deltacoronavirus Infecting HEK293 Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(5): 1587-1597.
[11]	CHEN Yongjie, LIU Jianxin, LI Huizi, ZHONG Wenxia, LI Baojian, PI Molin, NING Zhangyong. Inhibitory Effect of Stimulator of Interferon Genes on the Proliferation of Orf Virus in OFTu Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(2): 588-596.
[12]	JIA Hanxiao, YUAN Honggen, LI Zhen, GUAN Shuaiyin, ZHANG Jinhua, SONG Yunfeng. Screening of Proteins Interacting with PCV2 Replication Initiation Region and Study on the Regulation of Virus Replication by PARP1 Protein [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(10): 3511-3521.
[13]	DUAN Zhiqiang, XIE Lingling, CHEN Jiaqi, TANG Hong, WANG Yanbi, ZHAO Caiqin, ZHAO Jiafu. Research Progress on the Interactions of Newcastle Disease Virus M Protein with Host Proteins [J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(1): 32-42.
[14]	CHEN Sheng, LIAO Zhihong, XIE Zi, CHEN Feng, XIE Qingmei, SHU Wei. GADD45β Suppresses the Replication of Avian Leukosis Virus Subgroup J in DF-1 Cells [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(6): 1662-1669.
[15]	QIAN Bang, LI Yanmin, ZHU Xueliang, ZHANG Xueyan, Niyokwishimira Alfred, DOU Yongxi, ZHANG Zhidong. Establishment of an iELISA Method for Detection of Antibody againist Peste des Petits Ruminants Virus Based on H Protein Epitope Peptide [J]. Acta Veterinaria et Zootechnica Sinica, 2021, 52(1): 144-153.