1株绵羊边界病病毒的分离鉴定与序列分析

doi:10.11843/j.issn.0366-6964.2014.03.013

Abstract

Abstract:

In order to investigate the prevalence status of border disease，serum samples were collected from one sheep flock in east China and tested through RT-PCR method with the 5′-UTR generic primers of pestivirus.One sheep was detected as positive infection of border disease virus (BDV) at different sampling times.Positive serum sample was inoculated onto MDBK cells for viral isolation and identification.Then RT-PCR amplification of N^pro gene，electron microscopy detection of isolated virus，analysis of the complete genome sequences and phylogenetic analysis of the 5′-UTR and N^pro gene were performed.The results showed that no cytopathy effect (CPE) was observed until the eighth generation by serial passages.BDV was detected from cell cultures by RT-PCR using N^pro gene primers; and confirmed with sequence analysis as well as electron microscopy detection.The isolate was named as JSLS12-01.Amplification of complete genomic sequence was performed with six pairs of primers covering the near-full genome.The viral genome was about 12 227 nucleotides in size and had been submitted to the GenBank with registered number KC963426.Based on comparative sequence analysis of full genome and the deduced amino acids between this isolate and BDV reference strains，there were 72.3%-80.4% and 80.1%-89.7% identities，respectively.Phylogenetic analysis using partial 5′-UTR nucleotide sequence and N^pro sequence identified that the virus clustered within BDV 3 viruses，with the highest homology of 87.7% and 75.8%，respectively.Above all，we successfully isolated and identified one BDV 3 strain from sheep of the non-cytopathogenic biotype.

CLC Number:

S852.659.6

LIU Xia， MAO Li， LI Wen-liang， YANG Lei-lei， ZHANG Wen-wen, WEI Jian-zhong， JIANG Jie-yuan. Isolation and Sequence Analysis of a Border Disease Virus from Sheep[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2014, 45(3): 434-442.

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References

［1］LIU L，XIA H，WAHLBERG N，et al.Phylogeny，classification and evolutionary insights into pestiviruses［J］.Virology，2009，385(2):351-357.
［2］BECHER P，SCHMEISER S，OGUZOGLU T C，et al.Complete genome sequence of a novel pestivirus from sheep［J］.J Virol，2012，86(20):11412.
［3］BECHER P，ORLICH M，THIEL H J.Complete genomic sequence of border disease virus，a pestivirus from sheep［J］.J Virol，1998，72(6):5165-5173.
［4］BECHER P，MEYERS G，SHANNON A D，et al.Cytopathogenicity of border disease virus is correlated with integration of cellular sequences into the viral genome ［J］.J Virol，1996，70(5):2992-2998.
［5］OGUZOGLU T C，TAN M T，TOPLU N，et al.Border disease virus (BDV) infections of small ruminants in Turkey: a new BDV subgroup［J］.Vet Microbiol，2009，135(3-4):374-379.
［6］CABEZÓN O，ROSELL R，VELARDE R，et al.Border disease virus shedding and detection in naturally infected Pyrenean chamois (Rupicapra pyrenaica)［J］.J Vet Diagn Invest，2010，22(5):744-747.
［7］CABEZÓN O，VELARDE R，MENTABERRE G，et al.Experimental infection with chamois border disease virus causes long-lasting viraemia and disease in Pyrenean chamois (Rupicapra pyrenaica)［J］. J Gen Virol，2011，92(Pt 11):2494-2501.
［8］DUBOIS E，RUSSO P，PRIGENT M，et al.Genetic characterization of ovine pestiviruses isolated in France,between 1985 and 2006［J］.Vet Microbiol，2008，130(1-2): 69-79.
［9］YAZICI Z，SERDAR M S，GUMUSOVA S O，et al.Molecular diagnosis and seroepidemiology of pestiviruses in sheep［J］.Vet Arhiv，2012，82:35-45.
［10］李文良，毛立，张纹纹，等.从山羊持续性腹泻病例中分离到边界病病毒（BDV）［J］.江苏农业学报，2013，29(1):222-224.
［11］VILCEK S，HERRING A J，HERRING J A，et al.Pestiviruses isolated from pigs，cattle and sheep can be allocated into at least three genogroups using polymerase chain reaction and restriction endonuclease analysis［J］.Arch Virol，1994 ，136(3-4):309-323.
［12］VANTSIS J T，BARLOW R M，FRASER J，et al.Experiments in Border disease.Ⅷ.Propagation and properties of a cytopathic virus［J］.J Comp Pathol，1976，86(1):111-120.
［13］GIAMMARIOLI M，ROCCA S A L,STEINBACH F，et al.Genetic and antigenic typing of border disease virus (BDV) isolates from Italy reveals the existence of a novel BDV group［J］.Vet Microbiol，2011，147(3-4):231-236.
［14］THABTI F，LETELLIER C，HAMMAMI S，et al.Detection of a novel border ddisease virus subgroup in Tunisian sheep［J］.Arch Virol，2005，150(2):215-229.
［15］陆承平.兽医微生物学［M］.3版.北京：中国农业出版社，2001.
［16］LI W L，MAO L，ZHAO Y Q，et al.Detection of border disease virus(BDV) in goat herds suffering diarrhea in eastern China［J］.Virol J，2013，10:80-87.
［17］VALDAZO-GONZÁLEZ B，ALVAREZ M，SANDVIK T.Prevalence of border disease virus in Spanish lambs［J］.Vet Microbiol，2008，128 (3-4):269-278.
［18］FERNÁNDEZ-SIRERA L，CABEZÓN O，ALLEPUZ A，et al.Two different epidemiological scenarios of border disease in the populations of Pyrenean chamois (Rupicapra p.pyrenaica) after the first disease outbreaks［J］.PLoS ONE, 2012，7（12）：e51031.10.1371/journal.pone.0051031.
［19］DEPNER K R，HÜBSCHLE O J，LIESS B.Transplacental BVD virus transmission after experimental inoculation of goats in different pregnancy stages［J］.Dtsch tierarztl Wochenschr，1990，97(10):421-423.
［20］朱其太.边界病的研究进展［J］.甘肃畜牧兽医，1998(4):32-34.
［21］VALDAZO-GONZÁLEZ B，ALVAREZ-MARTÍNEZ M，GREISER-WILKE I.Genetic typing and prevalence of Border disease virus (BDV) in small ruminant flocks in Spain［J］. Vet Microbiol，2006，117(2-4):141-153.
［22］GARCÍA-PÉREZ A L，MINGUIJÓN E，ESTÉVEZ L，et al.Clinical and laboratorial findings in pregnant ewes and their progeny infected with Border disease virus (BDV-4 genotype)［J］.Res Vet Sci，2009，86(2):345-352.

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Isolation and Sequence Analysis of a Border Disease Virus from Sheep

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