Abstract:

In order to detect the expression of enJSRV and IFN-τ in endometrial tissue of Mongolian sheep as well as relationship of the expression of enJSRV and the variety of progesterone content in peripheral blood during early pregnancy. TaqMan Real-time Quantify PCR was performed on the relative expression of enJSRV and IFN-τ in uterine endometrium, as well as progesterone content in peripheral blood was detected by electrochemiluminescence analyses. The results demonstrated that enJSRV and IFN-τ mRNA were expressed specifically in uterine endometrium of Mongolian sheep. The mRNA expression level of enJSRV was high on Day 12-14, while lower than the former on Day 2-10 and 16-30 during pregnancy. IFN-τ mRNA was expressed on Day 12-25 of gestation with the maximum value on Day 14, and IFN-τ cannot be detected on Day 30. Electrochemiluminescence results showed the progesterone level was 0.4 ng·mL^-1 on Day 2 and rose afterwards on Day 8 of gestation, then kept at about 8.3 ng·mL^-1 on Day 8-16 and decreased during Day 19-30 with the value 2.5 ng·mL^-1 on Day 30. These results indicated that the expression of enJSRV mRNA in endometrium was highly correlated with changes in peripheral blood progesterone content and IFN-τ. Furthermore, enJSRV plays a significant role during placental morphogenesis and reproductive biology.