AA肉鸡和如皋黄鸡海马和下丘脑GR和FKBP5的表达及其与应激敏感性的关系

doi:10.11843/j.issn.0366-6964.2023.11.030

Abstract

Abstract: This study was conducted to investigate whether the expression of glucocorticoid receptor (GR) and FK-506 binding protein 5 (FKBP5) in hippocampus and hypothalamus of broiler chickens is related to stress sensitivity. Arbor Acres (AA) and Rugao yellow (Rugao) broiler chickens at 35 days of age were injected subcutaneously with corticosterone (CORT) for 10 consecutive days to establish a chronic stress model. The body weight, feed intake, behavior and plasma biochemical indexes of the two lines of chickens were detected, and the differences in stress sensitivity were compared. The expression of GR and FKBP5 in hippocampus and hypothalamus were detected by qPCR and Western blot, and their correlation with plasma CORT levels was analyzed. The results showed as follows: 1) After CORT treatment, the body weight of AA decreased significantly, the tonic immobility (TI) duration and plasma CORT level increased significantly, but there was no significant change in Rugao. CORT treatment resulted in increased heterophil/lymphocyte (H/L) ratio in blood of both AA and Rugao, yet H/L ratio was significantly higher in AA than in Rugao. 2) The basal level of GR protein expression in hippocampus and hypothalamus was significantly higher in Rugao than that in AA; The protein expression of GR was significantly down-regulated after CORT treatment, but Rugao still showed significantly higher GR protein than AA after stress. 3) CORT significantly up-regulated FKBP5 expression at both mRNA and protein levels in hippocampus and hypothalamus of AA, while the FKBP5 up-regulation in hippocampus and hypothalamus of Rugao only occurred at the protein level. FKBP5 protein expression was significantly in AA than in Rugao after stress. 4) Plasma CORT level was negatively correlated with GR protein expression in hippocampus and hypothalamus, and positively correlated with FKBP5 protein expression. GR was negatively correlated with FKBP5 protein expression in hippocampus and hypothalamus. These results indicate that AA and Rugao have different stress sensitivity, and the expression levels of GR and FKBP5 in hippocampus and hypothalamus are related to stress sensitivity.

Key words: broiler chicken, stress, hippocampus, hypothalamus, glucocorticoid receptor, FK-506 binding protein 5

CLC Number:

S852.21

LIU Jie, CONG Wei, ZHAO Mindie, ZHAO Ruqian. Expression of GR and FKBP5 in Hippocampus and Hypothalamus of Arbor Acres and Rugao Yellow Broiler Chickens and Its Relationship with Stress Sensitivity[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(11): 4766-4776.

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Expression of GR and FKBP5 in Hippocampus and Hypothalamus of Arbor Acres and Rugao Yellow Broiler Chickens and Its Relationship with Stress Sensitivity

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