Clade2.3.2 H5N1亚型禽流感疫苗候选株的构建

ACTA VETERINARIA ET ZOOTECHNICA SINICA ›› 2012, Vol. 43 ›› Issue (6): 915-921.doi:

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Generation of Clade 2.3.2 H5N1 Subtype Avian Influenza Vaccine Candidate

ZHANG Yan, ZHANG Wen-jun，LI Qun-hui，LIU Xiao-wen, LIU Wen-bo, LIU Xiu-fan^*

Key Laboratory of Animal Infectious Diseases of Ministry of Agriculture, Yangzhou University, Yangzhou 225009, China

Received:2011-10-13 Revised:1900-01-01 Online:2012-06-25 Published:2012-06-25
Contact: LIU Xiufan

Abstract

Abstract:

Currently, H5N1 viruses in Clade 2.3.2 are predominant viruses which prevail in China and other countries, and occurrence of a certain evolutionary trends of amino acid changes. In this report, a reassortant virus containing the HA and NA genes from an new isolate A/chicken /Yangzhou/1117/2011（YZC3） and the backbone A/Puerto Rico/8/34 (H1N1) (PR8) strain were constructed. The virus was rescued from the COS-1 cell line and prepared as inactivated wholevirus vaccine. Vaccine virus was avirulent to SPF chickens and embryonated chicken eggs, replicating well in eggs and MDCK cells. The results indicate that the reassortant viruses is suitable for vaccine manufacturing and being used as reference vaccine virus against the H5N1 Clade 2.3.2 viruses.

CLC Number:

S852.659.5

ZHANG Yan;ZHANG Wen-jun;LI Qun-hui;LIU Xiao-wen;LIU Wen-bo;LIU Xiu-fan. Generation of Clade 2.3.2 H5N1 Subtype Avian Influenza Vaccine Candidate[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2012, 43(6): 915-921.

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Generation of Clade 2.3.2 H5N1 Subtype Avian Influenza Vaccine Candidate

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