Abstract:

The extracellular gD gene was amplified by PCR from DEV genomic DNA according to our laboratory discovered gene sequence (GenBank accession number EU714267). The recombinant expression plasmid pET-30a-gDw could effectively express gDw fusion protein that was used to immunize rabbits. The titer of antibody tested by agar diffusion reaction was 1:16. Plaque reduction neutralization test showed that its neutralization antibody titer was up to 1:64. By indirected immunofluorescence, the results showed that gD appeared in the cell cytoplasm near the nuclear as early as 4 hours post infection. After 12 hours post infection, the fluorescence in cytoplasm was increased. Above all, the results afforded significant data for the study on the function of DEV gD.