FLI通过增加糖代谢途径促进牛卵母细胞体外成熟

doi:10.11843/j.issn.0366-6964.2025.06.022

Abstract

Abstract:

This study aimed to clarify the mechanism of FGF2, LIF and IGF1 (FLI) improving bovine oocyte maturation in vitro. The study used ovaries collected from slaughterhouses, cumulus oocyte complexes (COCs) extracted from ovaries were randomly divided into control group and FLI group for in vitro maturation, 30 COCs per group, each experiment was repeated 3 times. After 24 hours of in vitro maturation, first polar body excretion rate, cumulus expansion, mitochondrial membrane potential, cortical granule distribution, glucose consumption and pyruvate content in the medium, oocyte redox state, number of TZPs, reactive oxygen species content, expression of relevant genes were detected; the cleavage rate, blastocyst rate and blastocyst cell number of subsequent embryo development were counted. The results show that, the cumulus expansion index in FLI group was significantly higher than that in control group (3.16±0.04 and 2.43±0.02, respectively, P < 0.001). Compared with the control group, the expression of cumulus expansion related genes in oocytes of FLI group was increased (P < 0.05), increased migration of cortical granules to periovum (P < 0.05), however, the first polar body excretion rate and mitochondrial membrane potential of oocytes did not change significantly (P>0.05). Compared with the control group, the consumption of glucose and the content of pyruvate in the culture medium of FLI group were significantly increased (P < 0.05), NADPH in oocytes increased significantly (P < 0.001), and the expression of genes related to glucose metabolism showed significant up-regulation (P < 0.05). The addition of FLI could reduce the levels of redox, FAD++ and ROS in oocytes (P < 0.05). At IVM 8 h, the number of TZPs in FLI group was significantly higher than that in control group (106±6.91 and 78±8.76, respectively, P < 0.001). After in vitro fertilization, the cleavage rate and blastocyst rate in FLI group were significantly higher than those in control group ((86.49±0.80)%, (37.44±0.42)% and (74.08±0.91)%, (27.34±1.08)%, respectively, P < 0.05). The results show that, FLI can improve the quality of oocyte in vitro maturation and embryonic development by increasing glucose metabolism.

Key words: bovine, oocyte maturation in vitro, FLI, glycometabolism

CLC Number:

S823.3

HAN Xitong, ZHANG Nan, ZHANG Ning, ZHANG Jiaxin. FLI Promotes in Vitro Maturation of Bovine Oocytes by Increasing the Glucose Metabolism Pathway[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(6): 2778-2789.

Figures/Tables 11

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基因 Gene	引物序列(5′→3′) Primers sequence
PKM	F: CACGCAGAGACCATCAAGAA；R: TCGGATCTCAGGTCCTTTAGT
ACOD1	F: ACTGGAAATGGCTCTCCTCGG；R: CAAGTGGCAGCGTGGATTCTC
MPC1	F: TGAGCTCCGACTAAATGAGGA；R: TGGGATTGACTGCTGGGGGATA
GAPDH	F: GGGTCATCATCTCTGCACCT；R: GGTCATAAGTCCCTCCACGA
LDHA	F: TCTGGATTCAGCTCGCTTCCGTTA；R: TTCTTCAGGGAGACACCAGCAACA
HK1	F: GTGTGCTGTTGATAATCTCC；R: AATAACTGTTGGACGAATGC
GFPT1	F: AAACACAGTCGGCAGTTCCA；R: TGGCTACACCAATCTCAGGC
HAS2	F: CCTCATCATCCAAAGCCTGT；R: CGGGGTAGGTTAGCCTTTTC
TNFAIP6	F: CATCTTGCCACCTACAAGCA；R: CACACCACCACACTCCTTTG
β-actin	F: AACTCCATCATGAAGTGTGACG；R: GATCCACATCTGCTGGAAGG

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FLI Promotes in Vitro Maturation of Bovine Oocytes by Increasing the Glucose Metabolism Pathway

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