HE基因受体结合域变异对牛冠状病毒感染的影响

doi:10.11843/j.issn.0366-6964.2025.03.032

Abstract

Abstract:

To study the effect of HE gene receptor binding domain variation on bovine coronavirus (BCoV) infection, recombinant plasmids pEGFP-HE420, pEGFP-HE424 and pEGFP-HE428 were constructed. Three recombinant plasmids were transfected into HCT-8 cells respectively and then infected with NX-2021-B2-HE424 to detect viral mRNA expression levels at adsorption, internalization and replication stages by RT-qPCR, and to detect viral intracellular and extracellular titers by TCID₅₀. The results showed that pEGFP-HE420, pEGFP-HE424 and pEGFP-HE428 could be expressed in HCT-8 cells. Compared with pEGFP-HE424, deletion or insertion of HE gene receptor binding domain did not affect the adsorption, internalization, replication and intracellular virus titer. Deletion of HE gene receptor binding domain decreased extracellular titers of BCoV. HE gene receptor binding domain insertion did not affect the BCoV extracellular virus titer. In this study, three recombinant plasmids of HE genes with only receptor binding domain variation was constructed to explore its effect on bovine coronavirus infection in HCT-8 cells, providing data support for the study of the infection mechanism of BCoV.

Key words: bovine coronavirus, HE gene, receptor binding domain vibration, viral infection

CLC Number:

S852.659.6

JIANG Huihua, ZHAO Long, GUO Kangkang. Effect of HE Gene Receptor Binding Domain Variation on Bovine Coronavirus Infection[J]. Acta Veterinaria et Zootechnica Sinica, 2025, 56(3): 1336-1343.

Figures/Tables 8

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References 23

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引物名称 Primer name	引物序列(5′→3′) Primer sequence(5′→3′)
HE-F(Xho Ⅰ)	CCG$\underline{{\rm{CTCGAG}}}$ATGTTTTTGCTTCCTAGATTTG
HE-R(EcoR Ⅰ)	CG$\underline{{\rm{GAATTC}}}$GAGCATCATGCAGCCTAGTAC
Lig-HE-F	AAGGCTACTGTTTTGTCAAATACAAAGTATTATGATGATAGTC
Lig-HE-R	AACAGTAGCCTTAAACTTGCCATTAAAAATACAAAGTGGTAC
GAPDH-F(human)	ATTCCATGGCACCGTCAAGG
GAPDH-R(human)	TGGACTCCACGACGTACTCA
N-F	CGAGTTGAACACCCAGAT
N-R	GAGACGGGCATCTACACT

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Effect of HE Gene Receptor Binding Domain Variation on Bovine Coronavirus Infection

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