Acta Veterinaria et Zootechnica Sinica ›› 2024, Vol. 55 ›› Issue (9): 3978-3987.doi: 10.11843/j.issn.0366-6964.2024.09.022
• Animal Biotechnology and Reproduction • Previous Articles Next Articles
Yi WANG(), Jianfei GONG, Nuo HENG, Yingfan HU, Rui WANG, Huan WANG, Ni ZHU, Wei HE, Zhihui HU, Haisheng HAO, Huabin ZHU, Shanjiang ZHAO*(
)
Received:
2024-03-05
Online:
2024-09-23
Published:
2024-09-27
Contact:
Shanjiang ZHAO
E-mail:yiwang86@hotmail.com;zhaoshanjiang@caas.cn
CLC Number:
Yi WANG, Jianfei GONG, Nuo HENG, Yingfan HU, Rui WANG, Huan WANG, Ni ZHU, Wei HE, Zhihui HU, Haisheng HAO, Huabin ZHU, Shanjiang ZHAO. Melatonin Alleviates Palmitic Acid-induced Damage in Bovine Endometrial Epithelial Cells by Improving Mitochondrial Dynamics[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(9): 3978-3987.
Table 1
Primers in formation sequence of qRT-PCR"
引物名称 Primer name | GenBank accession No. | 引物序列(5′→3′) Sequence | 产物长度/bp PCR size |
β-Actin-Forward | NM_173979.3 | GCCCTGAGGCTCTCTTCCA | 101 |
β-Actin-Reverse | GCGGATGTCGACGTCACA | ||
MFN1-Forward | NM_001206508.1 | GAAAGCTGGGTGTCTTGTGC | 93 |
MFN1-Reverse | TCTGTGCCCGGACTGTCTA | ||
DRP1-Forward | NM_001046494.2 | GGCTGGGGTCTTGTGTGTTA | 141 |
DRP1-Reverse | TCTTTCCACTGCTCTGCGTT | ||
PGC1-α-Forward | NM_177945.3 | GACCACAAATGATGACCCTC | 90 |
PGC1-α-Reverse | TGGTTTGGCTTGTAGATGTT |
Fig. 6
The effect of melatonin on PA-induced mitochondrial dynamics-related proteins expression in BEECs A. The protein band plots of MFN1, p-DRP1/DRP1, PGC1-α, and β-actin in control, PA, and melatonin groups; B-D. The results of the analysis of protein expression of MFN1, p-DRP1/DRP1, and PGC1-α"
1 |
CASANOVA N , BEAULIEU K , FINLAYSON G , et al. Metabolic adaptations during negative energy balance and their potential impact on appetite and food intake[J]. Proc Nutr Soc, 2019, 78 (3): 279- 289.
doi: 10.1017/S0029665118002811 |
2 |
KRNJAIĆ S , CINCOVIĆ M , DJOKOVIĆ R , et al. The influence of energy balance, lipolysis and ketogenesis on metabolic adaptation in cows milked twice and three times daily[J]. Metabolites, 2022, 12 (11): 1090.
doi: 10.3390/metabo12111090 |
3 | 宋玉锡. 能量负平衡对奶牛泌乳早期繁殖性能和卵泡发育的影响[D]. 大庆: 黑龙江八一农垦大学, 2020. |
SONG Y X. Effects of negative energy balance on reproductive performance and follicular development during early lactation in dairy cows[D]. Daqing: Heilongjiang Bayi Agricultural University, 2020. (in Chinese) | |
4 |
CHANKEAW W , GUO Y Z , BÅGE R , et al. Elevated non-esterified fatty acids impair survival and promote lipid accumulation and pro-inflammatory cytokine production in bovine endometrial epithelial cells[J]. Reprod Fertil Dev, 2018, 30 (12): 1770- 1784.
doi: 10.1071/RD17537 |
5 |
FERST J G , GLANZNER W G , GUTIERREZ K , et al. Supplementation of oleic acid, stearic acid, palmitic acid and β-hydroxybutyrate increase H3K9me3 in endometrial epithelial cells of cattle cultured in vitro[J]. Anim Reprod Sci, 2021, 233, 106851.
doi: 10.1016/j.anireprosci.2021.106851 |
6 |
MEKURIAW Y . Negative energy balance and its implication on productive and reproductive performance of early lactating dairy cows: review paper[J]. J Appl Anim Res, 2023, 51 (1): 220- 228.
doi: 10.1080/09712119.2023.2176859 |
7 |
TIWARY S , NANDWANI A , KHAN R , et al. GRP75 mediates endoplasmic reticulum-mitochondria coupling during palmitate-induced pancreatic β-cell apoptosis[J]. J Biol Chem, 2021, 297 (6): 101368.
doi: 10.1016/j.jbc.2021.101368 |
8 |
WANG X H , ZHU M Y , LOOR J J , et al. Propionate alleviates fatty acid-induced mitochondrial dysfunction, oxidative stress, and apoptosis by upregulating PPARG coactivator 1 alpha in hepatocytes[J]. J Dairy Sci, 2022, 105 (5): 4581- 4592.
doi: 10.3168/jds.2021-21198 |
9 |
LI Y , DING H Y , LIU L H , et al. Non-esterified fatty acid induce dairy cow hepatocytes apoptosis via the mitochondria-mediated ROS-JNK/ERK signaling pathway[J]. Front Cell Dev Biol, 2020, 8, 245.
doi: 10.3389/fcell.2020.00245 |
10 |
VEZZA T , DÍAZ-POZO P , CANET F , et al. The role of mitochondrial dynamic dysfunction in age-associated type 2 diabetes[J]. World J Men's Health, 2022, 40 (3): 399- 411.
doi: 10.5534/wjmh.210146 |
11 |
CHIU Y H , LIN S C A , KUO C H , et al. Molecular machinery and pathophysiology of mitochondrial dynamics[J]. Front Cell Dev Biol, 2021, 9, 743892.
doi: 10.3389/fcell.2021.743892 |
12 |
GIACOMELLO M , PYAKUREL A , GLYTSOU C , et al. The cell biology of mitochondrial membrane dynamics[J]. Nat Rev Mol Cell Biol, 2020, 21 (4): 204- 224.
doi: 10.1038/s41580-020-0210-7 |
13 |
WANG J X , WU J N , LI W J , et al. Linking mitochondrial function to insulin resistance: focusing on comparing the old and the young[J]. Front Nutr, 2022, 9, 892719.
doi: 10.3389/fnut.2022.892719 |
14 |
CHEN D , LI X , ZHANG L T , et al. A high-fat diet impairs mitochondrial biogenesis, mitochondrial dynamics, and the respiratory chain complex in rat myocardial tissues[J]. J Cell Biochem, 2018, 119 (11): 9602.
doi: 10.1002/jcb.27068 |
15 |
HUANG A , SANCHES D . Abstract 4853:evaluating the role of melatonin in thyroid cancer cell (MDA-T41) apoptosis and metabolism modulation[J]. Cancer Res, 2023, 83 (7_Supplement): 4853.
doi: 10.1158/1538-7445.AM2023-4853 |
16 |
KOPUSTINSKIENE D M , BERNATONIENE J . Molecular mechanisms of melatonin-mediated cell protection and signaling in health and disease[J]. Pharmaceutics, 2021, 13 (2): 129.
doi: 10.3390/pharmaceutics13020129 |
17 |
ZHU Q , DING D , YANG H , et al. Melatonin protects mitochondrial function and inhibits oxidative damage against the decline of human oocytes development caused by prolonged cryopreservation[J]. Cells, 2022, 11 (24): 4018.
doi: 10.3390/cells11244018 |
18 |
NASONI M G , CARLONI S , CANONICO B , et al. Melatonin reshapes the mitochondrial network and promotes intercellular mitochondrial transfer via tunneling nanotubes after ischemic-like injury in hippocampal HT22 cells[J]. J Pineal Res, 2021, 71 (1): e12747.
doi: 10.1111/jpi.12747 |
19 |
AGIL A , CHAYAH M , VISIEDO L , et al. Melatonin improves mitochondrial dynamics and function in the kidney of zücker diabetic fatty rats[J]. J Clin Med, 2020, 9 (9): 2916.
doi: 10.3390/jcm9092916 |
20 | 王盼. 褪黑素对Aβ1-42介导AD模型保护作用的研究[D]. 沈阳: 中国医科大学, 2020. |
WANG P. Protective effect of melatonin in Aβ1-42 induced AD model[D]. Shenyang: China Medical University, 2020. (in Chinese) | |
21 | 郭攀. 褪黑素保护镉暴露所致肝毒性效应的机制探讨[D]. 重庆: 第三军医大学, 2015. |
GUO P. The mechanism study of protection effect of melatonin in cadmium induced hepatotoxicity[D]. Chongqing: Third Military Medical University of Chinese, 2015. (in Chinese) | |
22 | SUN B J , CHEN M H , ZHANG W H , et al. Palmitic acid induces GSDMD-mediated pyroptosis in periodontal ligament cells via the NF-κB pathway[J]. Oral Dis, 2023, 30 (4): 2546- 2557. |
23 |
KONAR S , HEDGES C P , CALLON K E , et al. Palmitic acid reduces viability and increases production of reactive oxygen species and respiration in rat tendon-derived cells[J]. bioRxiv, 2023,
doi: 10.1101/2023.02.08.527761 |
24 |
CHU S G , VILLALBA J A , LIANG X L , et al. Palmitic acid-rich high-fat diet exacerbates experimental pulmonary fibrosis by modulating endoplasmic reticulum stress[J]. Am J Respir Cell Mol Biol, 2019, 61 (6): 737- 746.
doi: 10.1165/rcmb.2018-0324OC |
25 |
CHOROSZY M , S'RODA-POMIANEK K , WAWRZYN'SKA M , et al. The role of palmitic acid in the co-toxicity of bacterial metabolites to endothelial cells[J]. Vasc Health Risk Manag, 2023, 19, 399- 409.
doi: 10.2147/VHRM.S408897 |
26 |
BRONIAREK I , KOZIEL A , JARMUSZKIEWICZ W . The effect of chronic exposure to high palmitic acid concentrations on the aerobic metabolism of human endothelial EA.hy926 cells[J]. Pflügers Arch-Eur J Physiol, 2016, 468 (9): 1541- 1554.
doi: 10.1007/s00424-016-1856-z |
27 | MERHAN O. Biochemistry and antioxidant effects of melatonin[M]//GELEN V, ŞENGÜL E, KÜKÜRT A. Melatonin-Recent Updates. Rijeka: IntechOpen. 2022: Ch. 3. |
28 |
JOSEPH T J , SCHUCH V , HOSSACK D J , et al. Melatonin: the placental antioxidant and anti-inflammatory[J]. Front Immunol, 2024, 15, 1339304.
doi: 10.3389/fimmu.2024.1339304 |
29 |
RUBIO-GONZÁLEZ A , REITER R J , DE LUXÁN-DELGADO B , et al. Pleiotropic role of melatonin in brain mitochondria of obese mice[J]. Melatonin Res, 2020, 3 (4): 538- 557.
doi: 10.32794/mr11250078 |
30 |
AGIL A , NAVARRO-ALARCON M , ALI F A Z , et al. Melatonin enhances the mitochondrial functionality of brown adipose tissue in obese—diabetic rats[J]. Antioxidants (Basel), 2021, 10 (9): 1482.
doi: 10.3390/antiox10091482 |
31 |
CHENG C T , KUO C Y , OUYANG C N , et al. Metabolic stress-induced phosphorylation of KAP1 Ser473 blocks mitochondrial fusion in breast cancer cells[J]. Cancer Res, 2016, 76 (17): 5006- 5018.
doi: 10.1158/0008-5472.CAN-15-2921 |
32 |
KNOTT A B , PERKINS G , SCHWARZENBACHER R , et al. Mitochondrial fragmentation in neurodegeneration[J]. Nat Rev Neurosci, 2008, 9 (7): 505- 518.
doi: 10.1038/nrn2417 |
33 |
DING M G , FENG N , TANG D S , et al. Melatonin prevents Drp1-mediated mitochondrial fission in diabetic hearts through SIRT1-PGC1α pathway[J]. J Pineal Res, 2018, 65 (2): e12491.
doi: 10.1111/jpi.12491 |
34 |
CHEN W R , ZHOU Y J , SHA Y , et al. Melatonin attenuates vascular calcification by inhibiting mitochondria fission via an AMPK/Drp1 signalling pathway[J]. J Cell Mol Med, 2020, 24 (11): 6043- 6054.
doi: 10.1111/jcmm.15157 |
35 | CHANG J Y A , YU F , SHI L H , et al. Melatonin affects mitochondrial fission/fusion dynamics in the diabetic retina[J]. J Diabetes Res, 2019, 2019, 8463125. |
36 | HU B , CHEN Z J , LIANG L L , et al. Melatonin promotes mitochondrial biogenesis and mitochondrial degradation in hepatocytes during sepsis[J]. Altern Ther Health Med, 2023, 29 (7): 284- 289. |
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