非洲猪瘟病毒MGF360-14L靶向MAVS抑制Ⅰ型干扰素的产生

doi:10.11843/j.issn.0366-6964.2022.09.041

Abstract

Abstract: This study was conducted to investigate the inhibitory effect of the African swine fever virus (ASFV) MGF360-14L on type Ⅰ interferon (IFN) and its mechanism. The effects of MGF360-14L on MAVS-induced IFN-β promoter activity were detected by dual luciferase assay, and the interaction between MGF360-14L and MAVS was examined by co-immunoprecipitation and indirect immunofluorescence assays. The results showed that the viral non-structural protein MGF360-14L inhibited interferon β (IFN-β) promoter activity induced by MAVS signaling. MGF360-14L interacted with MAVS and inhibited the phosphorylation of TBK1 and IRF3 induced by MAVS and TRIM21. In addition, MGFF360-14L competed with TRIM21 to bind MAVS and inhibit the TRIM21-mediated ubiquitination of MAVS, thereby reducing IFN-β levels. In conclusion, MGF360-14L may inhibit TRIM21-mediated ubiquitination of MAVS by competitively binding MAVS, thus down-regulating the production of type Ⅰ interferon. These findings provide new insights into the mechanisms underlying ASFV immune evasion.

Key words: African swine fever virus, type Ⅰ interferon, MAVS, ubiquitination, immune evasion

CLC Number:

S852.659.1

WANG Yang, CUI Shuai, XIN Ting, WANG Xixi, YU Hainan, CHEN Shiyu, JIANG Yajun, GAO Xintao, PANG Zhongbao, JIANG Yitong, GUO Xiaoyu, JIA Hong, ZHU Hongfei. ASFV MGF360-14L Interacts with MAVS and Inhibit the Expression of Type Ⅰ Interferon[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 3272-3278.

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ASFV MGF360-14L Interacts with MAVS and Inhibit the Expression of Type Ⅰ Interferon

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