鼠伤寒沙门菌小RNA GcvB靶基因的预测与验证

doi:10.11843/j.issn.0366-6964.2020.04.027

Abstract

Abstract: In order to screen the target genes regulated by Salmonella typhimurium small RNA GcvB, this study predicted genes that can interact with GcvB R1, R2 and R3 regions by TargetRNA2 software based on the transcriptome sequencing results of Salmonella typhimurium gcvB knockout strain, annotated the KEGG database and GO enrichment analysis and validated the predicted genes using real-time PCR. The predicted results of Target RNA2 showed that the narY, yeaK, trpB and STM2732 genes with the highest hybridization energy were able to produce at least 7 consecutive base complementary to the small RNA GcvB. NarY, YeaK and TrpB were respectively related to anaerobic respiration, tRNA that recognizes proline, and synthesis of tryptophan. The results of real-time PCR showed that the transcript levels of narY, yeaK, trpB and STM2732 were up-regulated by 3.4, 9.8, 12.1 and 18.37 times, respectively, under gcvB gene knockout conditions. These results indicated that the narY, yeaK, trpB and STM2732 genes were negatively regulated by small RNA GcvB and may be directly negatively regulated. This study laid the foundation for further understanding of the interaction between small RNA GcvB and target genes, the regulation mechanism of small RNA, and the pathogenic mechanism of Salmonella.

Key words: Salmonella, small RNA GcvB, transcriptome sequencing, target genes

CLC Number:

PAN Yong, LIU Lijuan, YANG Yang, LI Chen, MA Guangqiang, YANG Qi. Prediction and Validation of Small RNA GcvB Target Gene of Salmonella Typhimurium[J]. Acta Veterinaria et Zootechnica Sinica, 2020, 51(4): 894-898.

References 14

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Prediction and Validation of Small RNA GcvB Target Gene of Salmonella Typhimurium

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