鸭瘟病毒新近分离毒株部分基因变异分析

doi:10.11843/j.issn.0366-6964.2018.10.017

Abstract

Abstract:

The purpose of this study was to explore the infection status and molecular characteristics of duck plaque virus (DPV) currently circulating in ducks. The duck samples suspected to be infected with DPV were collected from Fujian province during 2016-2017, and were used for virus detection, isolation and gene sequences analysis. A total of 24 DPV strains were isolated from these samples, and the median age (Me) of the confirmed DPV-positive ducks were different among duck species. The Me of DPV-positive Mule duck and Muscovy duck were 90-day old and 88-day old, respectively, while the Me of DPV-positive Sheldrake duck was 287-day old. Sequence analysis showed that UL56/LORF5 gene, TK/gH gene and UL2 gene of the 24 isolates shared as high as more than 99% nucleotide homology with those of Chinese DPV reference virulent strains,and there exist regular nucleotide variations among the UL56/LORF5 gene and the TK gene between the current isolates and the Chinese reference virulent strains. Phylogenetic analysis showed that all of the current DPV isolates were gathered into one phylogenetic branch (Group Ⅱ) which different from the Chinese reference virulent strains (Group Ⅰ), and could further be divided into two sub-branches (Group Ⅱa and Group Ⅱb). The above results indicated that DPVs still circulated in ducks with Me-disparity among duck species, carrying some regular mutations compared to the Chinese reference virulent strains. We should strengthen the regular molecular epidemiological surveillance, and be aware of the epidemic variation of the virus. This will help for the rapid and accurate diagnosis and disease control of duck plague in China.

CLC Number:

S852.659.1

FU Guang-hua, CHEN Cui-teng, LIU Rong-chang, LU Li-zhi, SHI Shao-hua, JIANG Bin, FU Qiu-ling, CHENG Long-fei, SHEN Jun-da, TIAN Yong, WAN Chun-he, CHEN Hong-mei, HUANG Yu. Genetic Variability of Partial Genes of Duck Plaque Viruses Currently Isolated in Ducks[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2018, 49(10): 2215-2222.

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Genetic Variability of Partial Genes of Duck Plaque Viruses Currently Isolated in Ducks

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