MDV感染B19、B21单倍型SPF鸡外周血淋巴细胞BLec1、BNK及细胞因子基因转录水平比较

doi:10.11843/j.issn.0366-6964.2015.07.015

Abstract

Abstract:

This study was undertaken to reveal the possible resistance mechanism of B19 and B21 haplotype SPF chickens against MDV.One day-old B19 and B21 haplotype chickens were infected with MDV Md5 strain by intramuscular as 800 PFU per feather.The transcription levels of targets genes including BNK，BLec1，IFN-γ，IL-4，IL-10 and IL-18 were measured at different time points after MDV treatment.Results showed that gene transcription levels of BNK，BLec1 and the four cytokines in B19 and B21 chickens decreased compared to the control group on the 4th and 7th day post infection (dpi).On the 10th dpi，BNK，BLec1， IFN-γ，IL-4 and IL-18 gene transcriptions highly increased in B21 chicken in different levels，while decreased in B19 chickens.On the 13th dpi，gene transcriptions of BNK，BLec1，IL-18 and IL-10 in MDV treated B21 chickens also rose in different levels compared to the control group，while decreased in MDV treated B19 chickens.In conclusion，the present study demonstrate that B21 haplotype SPF chickens express high levels of BNK，BLec1 and cytokines than B19 ones and the resistant difference against MD between MD tolerant and sensitive chickens is related to the gene transcriptions of BNK， BLec1 and cytokines.

CLC Number:

NING Guan-bao，TIAN Wen-xia，ZHANG Yuan-hua，ZHANG Ding，ZHANG Xiao-na，HAN Ling-xia. Gene Transcription Comparison of BLec1，BNK and Cytokines in PBLs of B19 and B21 Haplotype SPF Chickens Infected with MDV[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(7): 1184-1190.

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Gene Transcription Comparison of BLec1，BNK and Cytokines in PBLs of B19 and B21 Haplotype SPF Chickens Infected with MDV

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