超强马立克病病毒株的致病性研究

doi:10.11843/j.issn.0366-6964.2015.07.016

Abstract

Abstract:

In this study，the pathogenicity of a very virulent Marek’s disease virus (MDV) SD2012-1 strain was analyzed.sixty SPF chickens were randomly divided into 3 groups：non-immunized group，HVT immunized group and CVI988 immunized group.Chickens were vaccinated with different types of MD vaccine at 1-day-old，and were challenged with SD2012-1 strain at 10-day-old.The challenged chickens were housed in separate isolators，and were sampled for clinic，histopathology and PCR detection of MDV.Clinical and histopathologic lesions in the infected chickens showed：slight histopathologic lesions were developed at 2 weeks post challenge，characteristic histopathologic lesions with scattered clumps of tumor cells were developed at the 6th weeks post challenge，and grossly obvious tumor lesions with large number of tumor cell aggregates were developed at the 9th weeks post challenge；SD2012-1 could break through the protection of CVI988 vaccine，causing MD with up to 30% rate in immunized chickens.PCR tracking of MDV in the infected chickens showed：MDV could be detected in non-immunized group and HVT group at 5 days post challenge；the positive rates of MDV were 100% in non-immunized and HVT group，30% in CVI988 group，at 10 days post challenge；the positive rates in the three groups were all 100% at 20 days post challenge；samples，including liver，kidney，muscular stomach，mesentery，duodenum，heart and bursa of Fabricius collected from clinical chickens，were all MDV positive by PCR，feather pulp samples collected from the infected living chickens were all MDV positive by PCR.When infected with MDV SD2012-1，chickens immunized with HVT hardly had protective effect on this virus，and the long-term existence of SD2012-1 in chickens immunized with CVI988 vaccine could break through its immune protection，which proposed a severe challenge for Marek’s disease prevention and control.

CLC Number:

S852.659.1

GONG Zhen-hua，ZHANG Kang，WANG Li-ping，GUO Guang-li，LI Jin-ping，YU Jian-min，LI Lei，HOU Guang-yu，WANG Jian-lin，SHAN Hu. Studies on Pathogenicity of a Very Virulent Marek’s Disease Virus Strain[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2015, 46(7): 1191-1200.

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Studies on Pathogenicity of a Very Virulent Marek’s Disease Virus Strain

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