新型凝集技术快速检测鸡白痢、鸡伤寒沙门菌

doi:10.11843/j.issn.0366-6964.2013.12.020

Abstract

Abstract:

The aim of this study was to develop a new agglutination test for the rapid detection of Salmonella pullorum and Salmonella gallinarum（S. pullorum and S. gallinarum）. In this study, three kinds of organic reactive dye and antibody were used to modify silica nanoparticles to prepare the immune colored silica nanoparticles (ICSN), ICSN were used to build new agglutination test for rapid detection of S. pullorum and S. gallinarum. The colored silica nanoparticles (CSN) were synthesized by reverse microemulsion method. The morphology and degree of dispersion of CSN were characterized by SEM. The agglutination test was used to characterize the detection effect of S. pullorum and S. gallinarum. The results showed that the new agglutination test was very sensitive, the aggregation results were prominently, intuitive and easy to distinguish by naked eye, moreover, consumption of antibody in new agglutination test is only 1/500 of that consumed in traditional test, the linear range for S. pullorum and S. gallinarum was from 10² to 10⁹ cfu·mL^-1. It was very stable and repeatable, after being stored at 4 ℃ for 28 days, the aggregation effect showed no significant difference; It also showed specificity and accuracy. The new agglutination test was simple, fast, accurate, sensitive and economic. This new agglutination test could not only be used for detecting S. pullorum and S. gallinarum rapidly, but also provide a basis model for rapid detection of other pathogenic bacterium.

CLC Number:

YANG Lei, ZHAO Guang-ying, DOU Wen-chao. The New Agglutination Test for Rapid Detection of Salmonella pullorum and Salmonella gallinarum[J]. ACTA VETERINARIA ET ZOOTECHNICA SINICA, 2013, 44(12): 1994-1999.

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The New Agglutination Test for Rapid Detection of Salmonella pullorum and Salmonella gallinarum

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