替拉扎明对多重耐药沙门菌抗菌增敏活性分析

doi:10.11843/j.issn.0366-6964.2023.10.032

Abstract

Abstract: The aim of this study was to explore the antibacterial mechanism of tirapazamine (TPZ) on Salmonella. We screened three multi-drug resistant Salmonella strains through drug sensitivity test, and measured the minimum inhibitory concentration (MIC), minimum bactericidal concentration (MBC), antibacterial effect, time bactericidal curve and biofilm formation ability of TPZ against multi-drug resistant Salmonella. The growth and proliferation of TPZ on Salmonella, the effects of drug resistance phenotype and biofilm formation were analyzed. The results showed that TPZ had obvious antibacterial effect on multi-drug resistant Salmonella, and the MIC values respectively of Salmonella strains ST, ST-6 and ST-7 were 12.5, 25 and 25 μmol·L^-1. MBC value was 50 μmol·L^-1. With the increases of the action concentration and prolongation of the action time of TPZ, the growth of Salmonella was significantly inhibited after co-culture for 4 hours, the cultivation of 50 μmol·L^-1 of TPZ was co-cultured with Salmonella ST, ST-6 and ST-7 for 8, 20 and 20 h respectively, and all Salmonella was killed; TPZ significantly reversed the drug resistance phenotype of multi-drug resistant Salmonella; TPZ had a significant inhibitory effect on the formation ability and amount of Salmonella biofilm (P<0.0001). TPZ had obvious antibacterial effect on multi-drug resistant Salmonella, and it could reverse the multi-drug resistance phenotype and reduce the formation of biofilm. The results of the study elucidated the antibacterial activity of TPZ against multi-drug resistant Salmonella, and new therapeutic drugs had been provided for the treatment and control of infections caused by Salmonella infection.

Key words: tirapazamine, multi-drug resistant Salmonella, biofilm, resistance phenotype, antibacterial activity

CLC Number:

WU Zhouhui, WANG Yu, DU Heng, WANG Zhiwen, XIAO Shuang, WU Jinliang, WANG Zhen. Analysis of the Antibacterial Sensitization Activity of Tirapazamine against Multi-Drug Resistant Salmonella[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(10): 4362-4371.

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Analysis of the Antibacterial Sensitization Activity of Tirapazamine against Multi-Drug Resistant Salmonella

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