A型产气荚膜梭菌感染鸭回肠代谢组学分析

doi:10.11843/j.issn.0366-6964.2023.06.033

Abstract

Abstract: The aim of this study was to explore the changes of different metabolites in duck ileum infected with Clostridium perfringens type A (CpA) by non-targeted metabonomics. The 37-day-old healthy ducklings infected with CpA were selected for the experiment. After the CpA infection was successful, pathological observation and PCR detection were adopted, the metabonomics of duck ileum was detected by liquid chromatography-mass spectrometry (LC-MS) at 66, 90 and 114 h in the control group and CpA-infected group, and the potential differential metabolites and related metabolic pathways were screened and analyzed. Finally, the differential metabolites in the control group and CpA-infected 114 h group were selected for verification. The duck CpA infection model was successfully established by autopsy, pathological observation and pathogenic nucleic acid detection.There are some overlapping ileum samples in PCA, which suggests that there may be the same ionic compounds in the ileum of infected ducks and control ducks. There are 14, 16 and 24 kinds of total differential metabolites in ducks infected at 66, 90 and 114 h, respectively, and there are 13, 14 and 28 enriched metabolic pathways, mainly focusing on arachidonic acid metabolism, phenylalanine, tyrosine and tryptophan biosynthesis, tyrosine metabolism, etc. At the same time, the results of differential metabolites verification showed that the change trend of valine and alanine content in ileum after 114 h infection was consistent with the results of non-targeted metabolomics. To sum up, the pathogenic mechanism of CpA infection in ducks may regulate the synthesis of related metabolites by affecting the metabolic pathways of related amino acids.

Key words: Clostridium perfringens type A, duck, metabonomics

CLC Number:

S852.616.3

ZENG Chengrong, WANG Na, BI Wenwen, MEI Shihui, HE Guangxia, ZHANG Junjie, CHEN Ze, WEN Ming, ZHOU Bijun. Metabonomics Analysis of Duck Ileum Infected by Clostridium perfringens Type A[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2555-2569.

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Metabonomics Analysis of Duck Ileum Infected by Clostridium perfringens Type A

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