Acta Veterinaria et Zootechnica Sinica ›› 2022, Vol. 53 ›› Issue (6): 1945-1957.doi: 10.11843/j.issn.0366-6964.2022.06.028

• CLINICAL VETERINARY MEDICINE • Previous Articles     Next Articles

Protective Effect of Sulfated Codonopsis pilosula Polysaccharide on RAW264.7 Cell Damage Induced by H2O2 and Acute Alcohol Injury in Mice

SUN Jiaqi1, YANG Shijing1, TAN Ying1, BAI Lin1, GUO Shining1,2, SHI Dayou1,2*, LIU Cui1*   

  1. 1. College of Veterinary Medicine, South China Agricultural University, Guangzhou 510642, China;
    2. Guangdong Technology Research Center for Traditional Chinese Veterinary Medicine and Nature Medicine, Guangzhou 510642, China
  • Received:2021-06-20 Online:2022-06-23 Published:2022-06-25

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Abstract: The aim of this study was to compare the immunoprotective effects of Codonopsis pilosula polysaccharide (CP) and sulphated Codonopsis pilosula polysaccharide (SCP) on acute injury induced by alcohol in mice and H2O2 in RAW264.7 cells. RAW264.7 cells was induced by H2O2 to build a cellular model of acute injury, and the effects of different concentrations of CP and SCP on the growth, phagocytosis, and IL-2, IL-4, IL-10, IFN-γ levels of RAW264.7 cells were detected. In addition, a mouse model of acute injury induced by alcohol was established in vivo. A total of 90 4-week-old Kunming male mice were randomly divided into 9 groups: blank group (B), model control group (MO), Vitamin C control group (VC), 3 doses of CP (CPL, CPM, CPH) and SCP (SCPL, SCPM, SCPH) groups. The effects of each group on the growth, blood physiological indexes, organ index, serum IL-2, IL-4, IL-10 and IFN-γ levels of acute injury mice were detected. The results showed that in the H2O2-induced cell inflammation model, compared with the model group, the number of adherent cells and the cell density in CP and SCP groups remarkably increased, and the phagocytosis ability of cells was significantly improved after 30, 60 and 90 min treatment at 37 ℃ (P < 0.05). Moreover, SCP groups showed better effects of promoting cell proliferation and phagocytosis. Both CP and SCP could significantly promote the secretion of IL-2 and IFN-γ (P < 0.05), and inhibit the secretion of IL-4 (P < 0.05). SCP groups had better inhibition effect on IL-4 than CP groups, and SCPL group had the best inhibition effect on IL-4. In acute injury model induced by alcohol, compared with model group, both CP and SCP could significantly improve the blood physiological indexes of mice (P < 0.05), and restore the WBC and HGB to normal levels, among which SCPL group had the best effect. CP and SCP could also improve serum biochemical indexes, significantly increase the contents of TC and LDH in serum (P < 0.05), and reduce the content of TG (P < 0.05). The serum levels of IL-2 and IFN-γ in CP and SCP groups were significantly increased (P < 0.05) compared with MO group, and the level of IL-4 was significantly decreased (P < 0.05), in which SCP groups had the best inhibition effect on IL-4. In conclusion, SCP significantly increased the phagocytosis ability of RAW264.7 cells after acute injury induced by H2O2, promoted the secretion of IL-2 and IFN-γ, and inhibited the secretion of IL-4. SCP, which had protection effect, can improve blood physiological indexes, serum biochemical indices and immune factors of acute injury mice induced by alcohol. Under certain conditions, the protective effect of SCP was better than CP.

Key words: Codonopsis pilosula polysaccharide, sulfate, acute injury, immune, cytokines

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