桥连双苯丙氨酸二肽增强灭活猪丁型冠状病毒在小鼠上的免疫效果分析

doi:10.11843/j.issn.0366-6964.2023.04.022

Abstract

Abstract: Porcine deltacoronavirus (PDCoV) can cause diarrhea, vomiting and even lead to death of newborn piglets, which has serious harm to pig breeding. Phenylalanine dipeptide and its derivatives can be self-assembled into nanometer supramolecular assemblies, which have the function of loading and delivering drugs. This study was conducted to explore whether bridging diphenylalanine dipeptide can enhance the immunogenicity of PDCoV. Firstly, its cytotoxicity was evaluated. Then, it was used as adjuvant (0.1 mg·mL^-1) and mixed with equal volume of inactivate PDCoV (PDCoV HNZK-02, TCID₅₀=10⁸·0.1 mL^-1) to prepare inactivated vaccine. Mice (200 μL per mouse) were immunized with inactivated vaccine, and the specific IgG antibody was detected by ELISA method, the SI index of lymphocyte proliferation was detected by CCK-8 kit, and the activity of specific cytotoxic T lymphocytes (CTL) was detected by lactate dehydrogenase (LDH). After 5 weeks of immunization, mice were challenged with PDCoV (500 μL per mouse) by intragastric administration, and the viral load of ileum and other tissues was detected 3 days later. The results showed that bridged diphenylalanine dipeptide had no obvious toxic effect on porcine testicular cells. Mice immunized with inactivated PDCoV with bridging diphenylalanine dipeptide could produce high level of IgG, SI and CTL were higher than those of the inactivated PDCoV group, and the intestinal tissue viral load was significantly lower than that of the inactivated PDCoV group. Bridged diphenylalanine dipeptide showed a good adjuvant effect and has the potential to become a new safe and effective adjuvant.

Key words: bridging diphenylalanine dipeptide, adjuvant, porcine deltacoronavirus, evaluation of immune

CLC Number:

S852.4

WANG Zi, WANG Nianxiang, TIAN Changming, ZHAO Fujie, LIU Lintao, MA Mengyao, JIA Xinhao, LIU Guoxing, ZHENG Lanlan. Using Mouse to Evaluate the Immune Effect of Bridged Diphenylalanine Dipeptide with Inactivated Porcine Deltacoronavirus[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(4): 1590-1597.

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Using Mouse to Evaluate the Immune Effect of Bridged Diphenylalanine Dipeptide with Inactivated Porcine Deltacoronavirus

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