一株鸭腺病毒3型的分离、鉴定及致病性分析

doi:10.11843/j.issn.0366-6964.2023.05.026

Abstract

Abstract: The purpose of this study was to isolate a novel Duck adenovirus 3 (DAdV-3) YN strain and elucidate its genetic characteristics and pathogenicity. LMH cells were selected for virus isolation and purification, and then the virus genes, including hexon, penton base, fiber1, and fiber2 were sequenced and analyzed. Seven-days-old Muscovy ducks were infected by intramuscular injection in the legs, and the mental state of the ducks was observed. Besides, the body weight changes, necropsy characteristics of organs, histopathological changes, viral load of organs, cloaca swab detoxification, and serum biochemistry were recorded and analyzed. Furthermore, the transcription levels of apoptosis-related genes in the bursa and the spleen were measured to explore the effect of YN strain on immune function. The results showed that the weight gain of infected ducks was significantly inhibited (P<0.01, P<0.000 1), and different degrees of lesions appeared in various organs. The coefficients of livers and kidneys of the experimental group were larger than those of the control group, and the difference was extremely significant on the 7th day after the challenge. Alanine aminotransferase, aspartate aminotransferase, and urea nitrogen in the serum of the Muscovy ducks in the challenge group were significantly higher than those in the control group (P<0.001, P<0.0001). At the same time, the mRNA levels of apoptosis-related genes in the bursa and the spleen increased significantly (P<0.01, P<0.001). (Conclusion) The above results suggest that DAdV-3 YN strain can damage livers and kidneys when challenging the 7-days-old Muscovy ducks, simultaneously, it can affect the immune function of Muscovy ducks by causing apoptosis of cells in the bursa and the spleen.

Key words: duck adenovirus 3, genetic characteristics, pathogenicity, immune function

CLC Number:

S858.32

CAO Xiuyun, LIU Jiwen, TANG Zhihui, ZHENG Ziyi, YAN Liping, SONG Suquan. Isolation, Identification and Pathogenicity Analysis of a Duck Adenovirus Type 3[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 2050-2061.

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Isolation, Identification and Pathogenicity Analysis of a Duck Adenovirus Type 3

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