酵母对亚急性瘤胃酸中毒绵羊缓解效果及作用机制研究

doi:10.11843/j.issn.0366-6964.2022.05.015

Abstract

Abstract: This study was designed to explore the alleviating effect and mechanism of a strain of Saccharomyces cerevisiae and two strains of Diutina rugosa from rumen on subacute ruminal acidosis (SARA) in sheep. In order to provide strains and a theoretical basis for developing probiotics to prevent SARA in sheep. Sixty two-month-old weaned Dubo sheep (♂)×Hu sheep (♀) F₁ generations were selected as experimental animals. SARA was successfully induced in forty sheep by gradually increasing the diet concentrate-to-roughage ratio. SARA sheep were divided into 4 groups (n=10):SARA group;Saccharomyces cerevisiae ACCC 21162 (SC) group;Diutina rugosa N09 (DR1)group; Diutina rugosa N07 (DR2)group. All four groups were fed a diet with a concentrate to roughage ratio of 90:10. And SC,DR1 and DR2 groups were respectively gavaged with 100 mL Saccharomyces cerevisiae ACCC 21162,Diutina rugosa N09 and Diutina rugosa N07 after morning feeding.The control group (CON, n=10) was fed 50:50 diet at all times. The ruminal pH was measured on the 1, 3, 7, and 10 d after adding yeasts, respectively. On 10 d after adding yeasts, rumen microflora, abnormal metabolites, and blood gas were measured. The results showed that:1) Ruminal pH in the yeast groups was significantly higher than that in the SARA group on the 3rd day (P<0.05), and SARA was effectively relieved in yeast groups. 2) The optimum bacteria in SC and CON groups were Lactobacillus and unidentified Prevotellaceae, respectively, while in the other groups it was unidentified Ruminococcaceae. In the yeast groups, SC group had the highest richness index and DR2 group had the highest diversity index. 3) The rumen and blood lactic acid and rumen histamine in the yeast groups were significantly lower than those in the SARA group (P<0.05). 4) Blood pH and base excess in SC and DR2 groups were extremely significantly higher than those in the SARA group (P<0.01). The blood carbon dioxide partial pressure, total carbon dioxide, and bicarbonate concentration in the yeast groups were significantly higher than those in the SARA group (P<0.05). 5) There was a significantly positive correlation between ruminal pH and actual bicarbonate in the blood (P<0.05), and a significant negative correlation between rumen lactic acid and Chao 1 index of rumen microflora (P<0.05). These results indicated that three yeasts had the effects on improving the abundance of rumen microflora, reducing rumen and blood lactic acid, alleviating rumen inflammation and body acidosis.

Key words: subacute ruminal acidosis, yeast, rumen microbial community, inflammation

CLC Number:

S858.266.5

HAN Guohao, GAO Xiaosha, DUAN Jinwei, ZHANG Huiqin, ZHENG Yan, HE Jinxin, DING Jian’an, HUO Nairui, PEI Caixia, LI Hongli, GU Shaopeng. Study on Relieving Effect and Mechanism of Yeast on Subacute Ruminal Acidosis in Sheep[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(5): 1475-1485.

References 32

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Study on Relieving Effect and Mechanism of Yeast on Subacute Ruminal Acidosis in Sheep

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