临床腹泻猪空肠组织中肾素-血管紧张素系统(RAS)的表达变化及与肠道炎症的关系

doi:10.11843/j.issn.0366-6964.2024.02.032

Abstract

Abstract: In the process of modern large-scale pig breeding, whether it is the stress response caused by weaning, replacement and replacement, or bacterial and viral infections, it is very easy to affect the health of the digestive tract and cause intestinal inflammation with diarrhea as the main symptom. The expression of renin-angiotensin system (RAS) in jejunum tissues of pigs with clinical diarrhea was studied to explore the relationship between RAS and intestinal inflammation. The study was conducted on nursery pigs with clinical symptoms of diarrhea, and the pathological changes of jejunum tissues of diarrhea pigs were observed by histopathology, Western blot and RT-qPCR. To clarify the expression changes of RAS components, especially angiotensin-converting enzyme 2(ACE2), and explore the effects of RAS on intestinal inflammation injury in pigs. The results showed that the pro-inflammatory factors TNF-α and IL-1β were significantly increased in jejunum tissues of pigs with clinical diarrhoea piglets, while the anti-inflammatory factor IL-10 was not significantly changed. Histopathology showed that jejunum epithelial villi exfoliated, glandular hyperplasia and hemorrhagic sites. The mRNA and protein levels of ACE2 and MasR and the content of Ang (1-7) were decreased, while the protein levels of ACE and AT1R and the content of Ang II were increased. The results suggest that local jejunum RAS is activated in the jejunal tissue of pigs with clinical diarrhoea piglets. The activity of ACE-Ang II-AT1R axis was much higher than that of ACE2-ang (1-7)-MasR axis, and ACE2 was inferior in anti-inflammatory damage.

Key words: renin-angiotensin system, piglets, jejunum, clinical diarrhea, intestinal inflammation

CLC Number:

S852.23

CHEN Xueqing, LI Zhiqiang, WU Yulong, ZHANG Chonghao, ZHANG Yuanshu. Expression of Renin Angiotensin System (RAS) in Jejunum Tissues of Piglets with Clinical Diarrhea and Its Relationship with Intestinal Inflammation[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 751-758.

References

[1] COSNES J, GOWER-ROUSSEAU C, SEKSIK P, et al. Epidemiology and natural history of inflammatory bowel diseases[J]. Gastroenterology, 2011, 140(6):1785-1794. e4.
[2] HARMER D, GILBERT M, BORMAN R, et al. Quantitative mRNA expression profiling of ACE2, a novel homologue of angiotensin converting enzyme[J]. FEBS Lett, 2002, 532(1-2):107-110.
[3] 李志强, 闫书平, 纪晓霞, 等. 血管紧张素转换酶2在仔猪空肠中的分布表达及其与物质转运的关系[J]. 畜牧兽医学报, 2019, 50(8):1676-1684.
LI Z Q, YAN S P, JI X X, et al. Distribution and expression of angiotensin-converting enzyme 2 (ACE2) in the Jejunum of piglets and its relationship with substance transport[J]. Acta Veterinaria et Zootechnica Sinica, 2019, 50(8):1676-1684.(in Chinese)
[4] KHAJAH M A, FATEEL M M, ANANTHALAKSHMI K V, et al. Anti-inflammatory action of angiotensin 1-7 in experimental colitis[J]. PLoS One, 2016, 11(3):e0150861.
[5] POTDAR A A, DUBE S, NAITO T, et al. Altered intestinal ACE2 levels are associated with inflammation, severe disease, and response to anti-cytokine therapy in inflammatory bowel disease[J]. Gastroenterology, 2021, 160(3):809-822. e7.
[6] MENDOZA-TORRES E, OYARZÚN A, MONDACA-RUFF D, et al. ACE2 and vasoactive peptides:novel players in cardiovascular/renal remodeling and hypertension[J]. Ther Adv Cardiovasc Dis, 2015, 9(4):217-237.
[7] 刘颖, 王换换, 闫书平, 等. 大鼠非酒精性单纯性脂肪肝中肾素血管紧张素系统两条通路的相互作用研究[J]. 畜牧兽医学报, 2019, 50(11):2309-2317.
LIU Y, WANG H H, YAN S P, et al. Study on the interaction of two pathways of renin angiotensin system in rat nonalcoholic simple fatty liver[J]. Acta Veterinaria et Zootechnica Sinica, 2019, 50(11):2309-2317.(in Chinese)
[8] LAMBERT D W, CLARKE N E, TURNER A J. Not just angiotensinases:new roles for the angiotensin-converting enzymes[J]. Cell Mol Life Sci, 2010, 67(1):89-98.
[9] LI Z Q, WANG K, JI X X, et al. ACE2 suppresses the inflammatory response in LPS-induced porcine intestinal epithelial cells via regulating the NF-κB and MAPK pathways[J]. Peptides, 2022, 149:170717.
[10] 莫畏畏. 早期断奶仔猪腹泻的发生原因及预防措施[J]. 现代畜牧科技, 2022(12):83-85.
MO W W. Causes and preventive measures of diarrhea in early weaned piglets[J]. Modern Animal Husbandry Science & Technology, 2022(12):83-85.(in Chinese)
[11] 李燕名. 引起仔猪腹泻的因素与防控[J]. 畜牧兽医科技信息, 2022(12):157-159.
LI Y M. Factors causing diarrhea of piglets and its prevention and control[J]. Chinese Journal of Animal Husbandry and Veterinary Medicine, 2022(12):157-159.(in Chinese)
[12] POHL C S, MEDLAND J E, MACKEY E, et al.[LL] Early weaning stress induces chronic functional diarrhea, intestinal barrier defects, and increased mast cell activity in a porcine model of early life adversity[J]. Neurogastroenterol Motil, 2017, 29(11):e13118.
[13] HASHIMOTO T, PERLOT T, REHMAN A, et al. ACE2 links amino acid malnutrition to microbial ecology and intestinal inflammation[J]. Nature, 2012, 487(7408):477-481.
[14] YISIREYILI M, UCHIDA Y, YAMAMOTO K, et al. Angiotensin receptor blocker irbesartan reduces stress-induced intestinal inflammation via AT1a signaling and ACE2-dependent mechanism in mice[J]. Brain Behav Immun, 2018, 69:167-179.
[15] GARG M, BURRELL L M, VELKOSKA E, et al. Upregulation of circulating components of the alternative renin-angiotensin system in inflammatory bowel disease:a pilot study[J]. J Renin-Angiotensin-Aldosterone Syst, 2015, 16(3):559-569.
[16] BIAGIOLI M, MARCHIANÒ S, ROSELLI R, et al. GLP-1 mediates regulation of colonic ACE2 expression by the bile acid receptor GPBAR1 in inflammation[J]. Cells, 2022, 11(7):1187.
[17] YANG C B, YANG X J, LACKEYRAM D, et al. Expression of apical Na⁺-l-glutamine co-transport activity, B⁰-system neutral amino acid co-transporter (B⁰AT1) and angiotensin-converting enzyme 2 along the jejunal crypt-villus axis in young pigs fed a liquid formula[J]. Amino Acids, 2016, 48(6):1491-1508.

Expression of Renin Angiotensin System (RAS) in Jejunum Tissues of Piglets with Clinical Diarrhea and Its Relationship with Intestinal Inflammation

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