猪繁殖与呼吸综合征病毒N蛋白与核糖体蛋白S20相互作用对病毒复制的影响

doi:10.11843/j.issn.0366-6964.2023.01.027

Abstract

Abstract: The present study aimed to screen and identify host proteins that interact with the nucleocapsid protein (N protein) of pathogenic porcine reproductive and respiratory syndrome virus (PRRSV). In this experiment, we screened host proteins that interact with N protein and investigated their effects on PRRSV replication by constructing a porcine lung yeast cDNA library, yeast two-hybrid, immunoprecipitation, laser confocal, overexpression and interference assays. The results showed that N protein and ribosomal protein S20 (RPS20) interacted with each other in yeast cells. The overexpression and interference assays also provided preliminary evidence that RPS20 can affect the proliferation of PRRSV in cells. This study enriches the network of PRRSV-host factor interactions and lays the foundation for further studies on the biological functions of RPS20 protein during PRRSV infection.

Key words: yeast two-hybrid, PRRSV, N protein, ribosomal protein S20

CLC Number:

S852.659.6

WANG Junjun, SONG Huimin, LI Zhuowei, JIANG Ping, ZHOU Shuanghai, LI Huanrong, LIU Xuewei. Effect of Interaction between N Protein and Ribosomal Protein S20 of Porcine Reproductive and Respiratory Syndrome Virus on Virus Replication[J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(1): 293-303.

References 26

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Effect of Interaction between N Protein and Ribosomal Protein S20 of Porcine Reproductive and Respiratory Syndrome Virus on Virus Replication

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