日粮添加酵母硒对鸡肉线粒体氧化损伤及细胞凋亡的影响

doi:10.11843/j.issn.0366-6964.2022.09.023

Abstract

Abstract: The aim of this study was to investigate the effect of yeast selenium addition in the diet on mitochondrial oxidative damage and apoptosis during postmortem aging of white-feathered chickens. The breasts of chickens fed diets supplemented with different levels of yeast selenium were used as the research objects to determine the degree of mitochondrial oxidative damage, antioxidant enzyme activity, Caspase-3 and Caspase-9 activities, meat tenderness of chicken during postmortem aging. The results showed that during the postmortem aging process, reactive oxygen species (ROS) content, mitochondrial permeability transition pore (MPTP) opening degree, mitochondrial membrane permeability and the activities of Caspase-3 and Caspase-9 were significantly lower than those of the control group (P<0.05). Selenium content, cytochrome c (Cyt-c) reduction level, mitochondrial membrane potential and glutathione peroxidase (GSH-Px) activity were significantly higher than those of the control group (P<0.05). The total antioxidant capacity (T-AOC) of the yeast selenium-fed groups were significantly higher than that of the control group during early stage of postmortem aging (P<0.05), while it increased at other time points, but the difference was not significant (P>0.05). Moreover, the myofibrillar fragmentation index (MFI) of the yeast-selenium-fed groups was significantly lower than that of the control group during the postmortem aging process, and the corresponding shear force was significantly higher than that of the control group (P<0.05). Thus, yeast-selenium feeding inhibited cell apoptosis by decreasing oxidative damage to chicken mitochondria and ultimately weakened myofibril protein degradation.

Key words: chicken breast, yeast selenium, apoptosis, mitochondrial oxidation, tenderness

CLC Number:

S831.5

YANG Xue, YANG Chao, WANG Zhuo, TANG Defu, ZHANG Li, YU Qunli. Effect of Dietary Supplementation of Yeast Selenium on Mitochondrial Oxidative Damage and Apoptosis of Chicken[J]. Acta Veterinaria et Zootechnica Sinica, 2022, 53(9): 3079-3092.

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Effect of Dietary Supplementation of Yeast Selenium on Mitochondrial Oxidative Damage and Apoptosis of Chicken

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