奶牛生殖道微生物与繁殖及相关疾病的研究进展

doi:10.11843/j.issn.0366-6964.2024.03.007

Abstract

Abstract: Reproductive tract microbiome plays an important role in maintaining the reproductive tract health and reproductive efficiency of the host. The disruption of reproductive tract microbiome homeostasis may lead to a variety of reproductive diseases and cause huge losses to the animal husbandry economy. In recent years, some progress has been made in the research of reproductive tract microbiome of dairy cows, which is crucial to the prevention and control of postpartum reproductive diseases in dairy cows. Different physiological stages of breeding cows and postpartum reproductive tract microorganisms may affect their pregnancy ability. Therefore, this paper summarizes the composition of reproductive tract microbiota of healthy dairy cows, the changes in reproductive tract microbiota at different physiological stages and their correlation with the establishment of pregnancy in dairy cows, as well as the influence of reproductive microecology disturbance on dairy cow reproduction. The purpose of this review is to provide references for improving the reproductive efficiency of dairy cows and preventing and controlling reproductive tract diseases by microorganisms.

Key words: cows, reproductive tract microorganisms, pregnancy, inflammation, reproductive efficiency

CLC Number:

S823.91

SHEN Wenjuan, YANG Zhuo, ZHANG Xinrui, FU Yu, TAO Jinzhong. Research Progress of Microorganisms and Reproductive and Related Diseases in Dairy Cows Reproductive Tract[J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 924-932.

References

[1] CLEMMONS B A, DANTAS F G, FRANCO G A, et al.Vaginal and uterine bacterial communities in postpartum lactating cows[J].J Anim Sci, 2017, 95(S2):52-53.
[2] 张超楠, 索朗曲吉, 高腾云, 等.牛子宫微生物构成与疾病关系[J].中国畜牧兽医, 2023, 50(11):4655-4663.
ZHANG C N, SUO L Q G, GAO T Y, et al.Relationship between microbial composition and disease in bovine uterus[J].China Animal Husbandry & Veterinary Medicine, 2023, 50(11):4655-4663.(in Chinese)
[3] LAGUARDIA-NASCIMENTO M, BRANCO K M G R, GASPARINI M R, et al.Vaginal microbiome characterization of nellore cattle using metagenomic analysis[J].PLoS One, 2015, 10(11):e0143294.
[4] 刘勋.基于高通量测序的健康与子宫内膜炎奶牛阴道菌群差异性研究[D].长春:吉林农业大学, 2015.
LIU X.Diversity analysis of vaginal bacterial microbiota in healthy and endometritis dairy cows through high-throughput sequencing[D].Changchun:Jilin Agricultural University, 2015.(in Chinese)
[5] 王雨.16S rDNA测序技术分析患子宫炎奶牛阴道细菌的组成[D].青岛:青岛农业大学, 2017.
WANG Y.Analysis of vaginal bacterial composition in dairy cows with metritis based on 16S rDNA sequencing method[D].Qingdao:Qingdao Agricultural University, 2017.(in Chinese)
[6] SWARTZ J D, LACHMAN M, WESTVEER K, et al.Characterization of the vaginal microbiota of ewes and cows reveals a unique microbiota with low levels of lactobacilli and Near-Neutral pH[J].Front Vet Sci, 2014, 1:19.
[7] 高硕.高低产母猪授精12天后激素和生殖道微生物分析[D].南京:南京农业大学, 2016.
GAO S.Analysis of hormone and microflora in genital tract of high or low prolificacy sows 12 days post insemination[D]. Nanjing:Nanjing Agricultural University, 2016.(in Chinese)
[8] SRINIVASAN M, ADNANE M, ARCHUNAN G, et al.Significance of cervico-vaginal microbes in bovine reproduction and pheromone production-A hypothetical review[J].Res Vet Sci, 2021, 135:66-71.
[9] ZAMBRANO-NAVA S, BOSCÁN-OCANDO J, NAVA J.Normal bacterial flora from vaginas of Criollo Limonero cows[J].Trop Anim Health Prod, 2011, 43(2):291-294.
[10] 李炳志.奶牛子宫内膜炎菌群分析及微生态制剂对其疗效观察[D].杨凌:西北农林科技大学, 2008.
LI B Z.Analysis on microflora vagina of edometritis cows and the therapeutic efficacy of micro-ecological agent in treated it[D].Yangling:Northwest A&F University, 2008.(in Chinese)
[11] JAKOBSSON T, FORSUM U.Lactobacillus iners:a marker of changes in the vaginal flora?[J].J Clin Microbiol, 2007, 45(9):3145.
[12] CARNEIRO L C, CRONIN J G, SHELDON I M.Mechanisms linking bacterial infections of the bovine endometrium to disease and infertility[J].Reprod Biol, 2016, 16(1):1-7.
[13] STOUT M J, CONLON B, LANDEAU M, et al.Identification of intracellular bacteria in the basal plate of the human placenta in term and preterm gestations[J].Am J Obstet Gynecol, 2013, 208(3):226.e1-266.e7.
[14] ONDERDONK A B, DELANEY M L, DUBOIS A M, et al.Detection of bacteria in placental tissues obtained from extremely low gestational age neonates[J].Am J Obstet Gynecol, 2008, 198(1):110.e1-110.e7.
[15] MOORE S G, ERICSSON A C, POOCK S E, et al.Hot topic:16S rRNA gene sequencing reveals the microbiome of the virgin and pregnant bovine uterus[J].J Dairy Sci, 2017, 100(6):4953-4960.
[16] WESTERMANN S, DRILLICH M, KAUFMANN T B, et al.A clinical approach to determine false positive findings of clinical endometritis by vaginoscopy by the use of uterine bacteriology and cytology in dairy cows[J].Theriogenology, 2010, 74(7):1248-1255.
[17] MICHAEL R P.Hormonal steroids and sexual communication in primates[J].J Steroid Biochem, 1975, 6(3-4):161-170.
[18] OTERO C, DE RUIZ C S, IBAÑEZ R, et al.Lactobacilli and enterococci isolated from the bovine vagina during the estrous cycle[J].Anaerobe, 1999, 5(3-4):305-307.
[19] QUEREDA J J, BARBA M, MOCÉ M L, et al.Vaginal microbiota changes during estrous cycle in dairy heifers[J].Front Vet Sci, 2020, 7:371.
[20] COWLEY H M, HEISS G S.Changes in vaginal bacterial flora during the oestrous cycle of the mouse[J].Microb Ecol Health Dis, 1991, 4(4):229-235.
[21] 刘畅, 王军, 刘红羽, 等.肉牛黄体期和卵泡期阴道菌群结构的比较研究[J].中国兽医杂志, 2016, 52(8):16-17, 20.
LIU C, WANG J, LIU H Y, et al.Comparison of vaginal micro flora structure of beef cattle between luteal and follicular phase[J].Chinese Journal of Veterinary Medicine, 2016, 52(8):16-17, 20.(in Chinese)
[22] AULT T B, CLEMMONS B A, REESE S T, et al.Bacterial taxonomic composition of the postpartum cow uterus and vagina prior to artificial insemination1[J].J Anim Sci, 2019, 97(10):4305-4313.
[23] AAGAARD K, RIEHLE K, MA J, et al.A metagenomic approach to characterization of the vaginal microbiome signature in pregnancy[J].PLoS One, 2012, 7(6):e36466.
[24] 杜海东, 娜仁花.反刍动物妊娠期和泌乳期生理代谢和微生物变化及其对子代发育的影响研究进展[J].畜牧兽医学报, 2023, 54(11):4458-4467.
DU H D, NA R H.Research progress on physiological metabolism and microbial changes of ruminants during gestation and lactation and their effects on offspring development[J].Acta Veterinaria et Zootechnica Sinica, 2023, 54(11):4458-4467.(in Chinese)
[25] TULCHINSKY D, HOBEL C J, YEAGER E, et al.Plasma estrone, estradiol, estriol, progesterone, and 17-hydroxyprogesterone in human pregnancy.I.Normal pregnancy[J].Am J Obstet Gynecol, 1972, 112(8):1095-1100.
[26] MOR G, CARDENAS I.REVIEW ARTICLE:the immune system in pregnancy:a unique complexity[J].Am J Reprod Immunol, 2010, 63(6):425-433.
[27] CROZIER S R, ROBINSON S M, BORLAND S E, et al.Do women change their health behaviours in pregnancy?Findings from the Southampton Women's Survey[J].Paediatr Perinat Epidemiol, 2009, 23(5):446-453.
[28] MESSMAN R D, CONTRERAS-CORREA Z E, PAZ H A, et al.Vaginal bacterial community composition and concentrations of estradiol at the time of artificial insemination in Brangus heifers[J].J Anim Sci, 2020, 98(6):skaa178.
[29] FREITAS A C, CHABAN B, BOCKING A, et al.The vaginal microbiome of pregnant women is less rich and diverse, with lower prevalence of Mollicutes, compared to non-pregnant women[J].Sci Rep, 2017, 7(1):9212.
[30] ROMERO R, HASSAN S S, GAJER P, et al.Erratum to:the composition and stability of the vaginal microbiota of normal pregnant women is different from that of non-pregnant women[J].Microbiome, 2014, 2(1):10.
[31] GENÍS S, CERRI R L A, BACH À, et al.Pre-calving intravaginal administration of lactic acid bacteria reduces metritis prevalence and regulates blood neutrophil gene expression after calving in dairy cattle[J].Front Vet Sci, 2018, 5:135.
[32] DENG F L, MCCLURE M, RORIE R, et al.The vaginal and fecal microbiomes are related to pregnancy status in beef heifers[J].J Anim Sci Biotechnol, 2019, 10:92.
[33] NESENGANI L T, WANG J, YANG Y J, et al.Unravelling vaginal microbial genetic diversity and abundance between Holstein and Fleckvieh cattle[J].RSC Adv, 2017, 7(88):56137-56143.
[34] WANG Y, AMETAJ B N, AMBROSE D J, et al.Characterisation of the bacterial microbiota of the vagina of dairy cows and isolation of pediocin-producing Pediococcus acidilactici[J].BMC Microbiol, 2013, 13:19.
[35] 赵静.奶牛子宫内膜炎相关微生态菌群及其与宿主互作机制研究[D].长春:吉林农业大学, 2016.
ZHAO J.Study on cow endometritis associated microbiota and its interaction mechanisms with the host[D].Changchun:Jilin Agricultural University, 2016.(in Chinese)
[36] 李炳志, 赵献军, 马治宇, 等.健康与子宫内膜炎奶牛生殖道需氧及兼性厌氧细菌研究[J].西北农林科技大学学报:自然科学版, 2008, 36(11):57-60.
LI B Z, ZHAO X J, MA Z Y, et al.Difference of aerobic and facultative anaerobic bacteria in vagina of postpartum healthy cows and endometritis cows[J].Journal of Northwest A&F University:Natural Science Edition, 2008, 36(11):57-60.(in Chinese)
[37] WANG J, SUN C T, LIU C, et al.Comparison of vaginal microbial community structure in healthy and endometritis dairy cows by PCR-DGGE and real-time PCR[J].Anaerobe, 2016, 38:1-6.
[38] 王国卿.产后正常奶牛和子宫内膜炎患牛子宫内细菌的研究[D].哈尔滨:东北农业大学, 2000.
WANG G Q.The uterine bacteria in both postpartum normal and endometritis affected cows[D].Harbin:Northeast Agricultural University, 2000.(in Chinese)
[39] JEON S J, VIEIRA-NETO A, GOBIKRUSHANTH M, et al.Uterine microbiota progression from calving until establishment of metritis in dairy cows[J].Appl Environ Microbiol, 2015, 81(18):6324-6332.
[40] HUSSAIN A M, DANIEL R C W, O'BOYLE D.Postpartum uterine flora following normal and abnormal puerperium in cows[J].Theriogenology, 1990, 34(2):291-302.
[41] DIGIULIO D B, CALLAHAN B J, MCMURDIE P J, et al.Temporal and spatial variation of the human microbiota during pregnancy[J].Proc Natl Acad Sci U S A, 2015, 112(35):11060-11065.
[42] MORENO I, CODOÑER F M, VILELLA F, et al.Evidence that the endometrial microbiota has an effect on implantation success or failure[J].Am J Obstet Gynecol, 2016, 215(6):684-703.
[43] SANTOS T M A, GILBERT R O, BICALHO R C.Metagenomic analysis of the uterine bacterial microbiota in healthy and metritic postpartum dairy cows[J].J Dairy Sci, 2011, 94(1):291-302.
[44] 侯荣伦.奶牛生殖道菌群分析和乳酸菌的分离与鉴定及其生物学特性研究[D].呼和浩特:内蒙古农业大学, 2011.
HOU R L.Analysis of microbial flora in the dairy cow's genital tract and isolation and identification Lactobacillus and the biological characteristics of Lactobacillus[D].Hohhot:Inner Mongolia Agricultural University, 2011.(in Chinese)
[45] 凌宗欣.女性生殖道微生物群落菌群多样性变化与生殖道感染的相关性研究[D].杭州:浙江大学, 2012.
LING Z X.Diversity of urogenital microbiota associated with female lower genital tract infections[D].Hangzhou:Zhejiang University, 2012.(in Chinese)
[46] RAVEL J, GAJER P, ABDO Z, et al.Vaginal microbiome of reproductive-age women[J].Proc Natl Acad Sci U S A, 2011, 108(S1):4680-4687.
[47] PENG Y, WANG Y H, HANG S Q, et al.Microbial diversity in uterus of healthy and metritic postpartum Holstein dairy cows[J].Folia Microbiol (Praha), 2013, 58(6):593-600.
[48] 邓洪宇.嗜酸乳杆菌、鼠李糖乳杆菌、枯草芽孢杆菌复合活菌制剂防治奶牛子宫内膜炎的动物试验研究[D].大庆:黑龙江八一农垦大学, 2009.
DENG H Y.An experimental study of compound probiotics with Lactobacillus acidophilus, Lactobacillus rhamnosus and Bacillus subtilis in animal models of cow endometritis[D].Daqing:Heilongjiang Bayi Agricultural University, 2009.(in Chinese)
[49] MORENO C G, LUQUE A T, GALVÃO K N, et al.Bacterial communities from vagina of dairy healthy heifers and cows with impaired reproductive performance[J].Res Vet Sci, 2022, 142:15-23.
[50] SHELDON I M, LEWIS G S, LEBLANC S, et al.Defining postpartum uterine disease in cattle[J].Theriogenology, 2006, 65(8):1516-1530.
[51] LEBLANC S J, DUFFIELD T F, LESLIE K E, et al.Defining and diagnosing postpartum clinical endometritis and its impact on reproductive performance in dairy cows[J].J Dairy Sci, 2002, 85(9):2223-2236.
[52] SHELDON I M, PRICE S B, CRONIN J, et al.Mechanisms of infertility associated with clinical and subclinical endometritis in high producing dairy cattle[J].Reprod Domest Anim, 2009, 44(S3):1-9.
[53] LEBLANC S J, OSAWA T, DUBUC J.Reproductive tract defense and disease in postpartum dairy cows[J].Theriogenology, 2011, 76(9):1610-1618.
[54] SANTOS T M A, BICALHO R C.Diversity and succession of bacterial communities in the uterine fluid of postpartum metritic, endometritic and healthy dairy cows[J].PLoS One, 2012, 7(12):e53048.
[55] CHEN C, SONG X L, WEI W X, et al.The microbiota continuum along the female reproductive tract and its relation to uterine-related diseases[J].Nat Commun, 2017, 8(1):875.
[56] KUBYSHKIN A V, ALIEV L L, FOMOCHKINA I I, et al.Endometrial hyperplasia-related inflammation:its role in the development and progression of endometrial hyperplasia[J].Inflamm Res, 2016, 65(10):785-794.
[57] WILLIAMS E J, FISCHER D P, PFEIFFER D U, et al.Clinical evaluation of postpartum vaginal mucus reflects uterine bacterial infection and the immune response in cattle[J].Theriogenology, 2005, 63(1):102-117.
[58] TAKÁCS T, GÁTHY I, MACHÁTY Z, et al.Bacterial contamination of the uterus after parturition and its effect on the reproductive performance of cows on large-scale dairy farms[J].Theriogenology, 1990, 33(4):851-865.
[59] 彭宇.体外培养和分子生物学技术研究奶牛产后子宫内菌群的动态变化[D].南京:南京农业大学, 2011.
PENG Y.Dynamic changes of bacterial community in uterus of postpartum dairy cows by culture and molecular method[D]. Nanjing:Nanjing Agricultural University, 2011.(in Chinese)
[60] 刘超逊, 梁辛, 杨承剑, 等.健康水牛与患子宫内膜炎水牛子宫内细菌组成的比较分析[J].中国兽医学报, 2015, 35(1):116-121.
LIU C X, LIANG X, YANG C J, et al.Comparative analysis of uterine bacterial composition in healthy and endometritis buffaloes[J]. Chinese Journal of Veterinary Science, 2015, 35(1):116-121.(in Chinese)
[61] 孙城涛, 赵静, 王军, 等.健康与子宫内膜炎奶牛阴道菌群结构的比较研究[J].中国兽医学报, 2014, 34(4):622-627.
SUN C T, ZHAO J, WANG J, et al.The comparison of the vaginal bacterial microbiota in healthy and metritic dairy cows[J].Chinese Journal of Veterinary Science, 2014, 34(4):622-627.(in Chinese)
[62] 王国卿, 王洪海, 郭梦尧, 等.奶牛产后急性子宫内膜炎病原的分离与鉴定[J].中国兽医杂志, 2010, 46(10):10-12.
WANG G Q, WANG H H, GUO M Y, et al.Solation and identification on pathogenic microorganism from acute endometritis in postpartum dairy cows[J].Chinese Journal of Veterinary Medicine, 2010, 46(10):10-12.(in Chinese)
[63] 虞蕴如, 袁锦和, 徐魁梧, 等.青年母乳牛子宫内膜炎病原的研究[J].畜牧与兽医, 1991(1):6-8.
YU Y R, YUAN J H, XU K W, et al.Study on the pathogens of endometritis in young dairy cows[J].Animal Husbandry & Veterinary Medicine, 1991(1):6-8.(in Chinese)
[64] NI J L, WANG J, ZHAO K S, et al.Vaginal microbiome dynamics of cows in different parities[J].Animals (Basel), 2023, 13(18):2880.

Research Progress of Microorganisms and Reproductive and Related Diseases in Dairy Cows Reproductive Tract

PDF (PC)

Knowledge

Abstract

Cite this article

share this article

References

Related Articles 15

Recommended Articles

Metrics

Comments

[1]	ZHONG Zhuxia, HU Xiuzhong, XIANG Min, YU Jie, LIU Chenhui, ZHAO Shenglan, WAN Pingmin, WANG Dingfa, ZHOU Yuan, CHENG Lei. Research Progress on Biological Function and Application of Pregnancy Associated Glycoproteins in Livestock Production [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 874-881.
[2]	XIA Shuwen, CHEN Kunlin, SHEN Yangyang, AN Zhenjiang, ZHAO Fang, DING Qiang, ZHONG Jifeng, LIN Zhiping, WANG Huili. The Estimation of Genetic Parameters for Longevity Traits of Holstein Cows in Jiangsu Region [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(3): 1030-1039.
[3]	ZHANG Xinrui, FU Yu, YANG Zhuo, SHEN Wenjuan, TAO Jinzhong. Study of Early Pregnancy Diagnostic Proteins in Dairy Cows [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 451-460.
[4]	GAO Xin, SUN Yipeng. Research Progress of Cell Inflammation Induced by Influenza A Virus [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 481-490.
[5]	ZHANG Zhifei, TANG Xueying, MIN Li, TONG Xiong, CHEN Weidong, JU Xianghong, LI Dagang. Construction of Gene Coexpression Network Related to Lactation Period and Fecundity in Liver Tissue of Holstein Cows [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 528-539.
[6]	WANG Dong, LIU Kexin, HE Yanjun, DENG Shouxiang, LIU Yun, MA Weiming. Effects of Dietary Sodium Humate Supplementation on Liver Tissue Inflammation and Antioxidant Capacity of Salmonella Typhimurium-Infected Broilers [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 629-639.
[7]	CHEN Xueqing, LI Zhiqiang, WU Yulong, ZHANG Chonghao, ZHANG Yuanshu. Expression of Renin Angiotensin System (RAS) in Jejunum Tissues of Piglets with Clinical Diarrhea and Its Relationship with Intestinal Inflammation [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(2): 751-758.
[8]	YAO Ying, ZHOU Yingcong, DU Peiyan, LI Yijuan, QIAN Wenjie, LI Liuyang, YU Zhipeng, CUI Yan, YU Sijiu, FAN Jiangfeng. Proteomic Analysis of Yak Serum During Pregnancy Based on TMT Technology [J]. Acta Veterinaria et Zootechnica Sinica, 2024, 55(1): 192-206.
[9]	ZHONG Hua, SONG Shanshan, SHAO Huanting, ZHAO Yu, KANG Jinwen, WU Yao, SU Renwei. Transcriptome Sequencing Analysis on Canine Pyometra Uterine Tissue [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(8): 3383-3392.
[10]	ZHAO Wanli, CAO Qiqi, YANG Yue, DENG Zhaoju, XU Chuang. The Interaction between Gastrointestinal Microbiota and Mucosal Immunity in Health of Perinatal Dairy Cows [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(7): 2751-2760.
[11]	YUAN Kaimin, SHI Yuxin, DONG Zhihao, ZHAO Lingjun, XU Shiyuan, WU Kaihui, WANG Dong. Research Progress on Early Pregnancy Diagnosis Technologies in Cows [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2223-2230.
[12]	ZHANG Junxing, ZHANG Hailiang, HAN Liyun, MA Yanfen, WEN Wan, ZHOU Jiamin, TIAN Jia, LU Tingting, MA Yun, WANG Yachun. Analysis on the Influencing Factors of Wellness Traits in Holstein Lactating Cows in Ningxia [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(6): 2389-2401.
[13]	LI Wenqiao, LI Fadi, WANG Xinji, YUE Xiangpeng. Advance of Early Pregnancy Diagnosis in Livestock [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1782-1791.
[14]	HUANG Shangzhen, MA Longgang, LOU Wenqi, NING Jingyang, ZHANG Hailiang, HU Lirong, ZHA Qiong, LI Bin, XU Qing, BASANG Luobu, WANG Yachun. Analysis of Influencing Factors on Blood Indicators of Dairy Cows at High-altitude Area [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(5): 1964-1978.
[15]	FENG Xiaoyi, YANG Baigao, HAO Haisheng, DU Weihua, ZHU Huabin, CUI Kai, ZHAO Xueming. Mechanism and Solution of Heat Stress Induced Embryo Quality Decline in Dairy Cows [J]. Acta Veterinaria et Zootechnica Sinica, 2023, 54(3): 868-876.